Fluorinated boron nitride nanosheets as an inorganic matrix for the MALDI mass spectrometry analysis of perfluoroalkyl acids

A Simple and Rapid Quantitative Assay for Gossypol via Reactive Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

“…The high-performance liquid chromatography–mass spectrometry (HPLC-MS) approach is specific and sensitive but requires complex sample pretreatment, long run times, and highly trained personnel for operation, limiting its high-throughput application. Among different mass spectrometry techniques, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful technique engaged in a wide range of applications for food quality control and the medical field because of its simplicity, high salt tolerance, high throughput, and easy sample preparation. − However, there are still several challenges for the small molecules (<700 Da), such as matrix interference and lower ionization efficiencies of target compounds. , To reduce the background interference from organic matrices in low mass ranges, high molecular weight matrices, binary matrices, and inorganic matrices have been developed. − Chemical derivatization was always carried out to improve the sensitivity of the small molecules that were difficult to be ionize in MALDI-TOF MS. − However, the excessive derivatization reagents should be removed by an extra step. In recent years, reactive matrices that serve as both derivatization reagents and matrices to promote the desorption and ionization of the target compounds have been developed. , Utilizing reactive matrices not only improves the detection sensitivity and selectivity but also can simplify sample pretreatment.…”

Serum amino acids quantification by plasmonic colloidosome-coupled MALDI-TOF MS for triple-negative breast cancer diagnosis

“…However, there are two main limitations of the traditional MALDI-TOF MS for metabolic profiling. First, the traditional organic matrices, such as alpha-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB), show strong peaks by themselves in the low molecular weight (LMW) region (<500 ​Da), interfering the detection of metabolites [ 16 ]. Second, due to the "sweet spot" effect, the traditional MALDI process constitutes a stochastic element, which limits its reproducibility and performance in quantification [ 17 ].…”