Serum amino acids quantification by plasmonic colloidosome-coupled MALDI-TOF MS for triple-negative breast cancer diagnosis

Han, Xinyue; Li, Dandan; Wang, Shurong; Lin, Yuxiang; Liu, Yun; Lin, Ling; Qiao, Liang

doi:10.1016/j.mtbio.2022.100486

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…The aforementioned outcomes have laid a solid foundation for subsequent research endeavours. It is crucial to emphasize that our sample size of 38 vs 35 in metabolomic analysis is positioned at an intermediate level ( Chinello et al, 2010 ; Kriegsmann et al, 2015 ; Laguna et al, 2021 ; Han et al, 2022 ), yet remains sufficient for decoding the metabolic fingerprint features of atherosclerosis. Furthermore, our clinical samples (38 vs 35) were meticulously collected following strict admission and exclusion criteria (Experimental section).…”

Section: Resultsmentioning

confidence: 99%

Metabolomics unveils the exacerbating role of arachidonic acid metabolism in atherosclerosis

Ma,

He,

Liu

et al. 2024

Front. Mol. Biosci.

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Atherosclerosis is a complex vascular disorder characterized by the deposition of lipids, inflammatory cascades, and plaque formation in arterial walls. A thorough understanding of its causes and progression is necessary to develop effective diagnostic and therapeutic strategies. Recent breakthroughs in metabolomics have provided valuable insights into the molecular mechanisms and genetic factors involved in atherosclerosis, leading to innovative approaches for preventing and treating the disease. In our study, we analyzed clinical serum samples from both atherosclerosis patients and animal models using laser desorption ionization mass spectrometry. By employing methods such as orthogonal partial least-squares discrimination analysis (OPLS-DA), heatmaps, and volcano plots, we can accurately classify atherosclerosis (AUC = 0.892) and identify key molecules associated with the disease. Specifically, we observed elevated levels of arachidonic acid and its metabolite, leukotriene B4, in atherosclerosis. By inhibiting arachidonic acid and monitoring its downstream metabolites, we discovered the crucial role of this metabolic pathway in regulating atherosclerosis. Metabolomic research provides detailed insights into the metabolic networks involved in atherosclerosis development and reveals the close connection between abnormal metabolism and the disease. These studies offer new possibilities for precise diagnosis, treatment, and monitoring of disease progression, as well as evaluating the effectiveness of therapeutic interventions.

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Section: Resultsmentioning

confidence: 99%

Metabolomics unveils the exacerbating role of arachidonic acid metabolism in atherosclerosis

Ma,

He,

Liu

et al. 2024

Front. Mol. Biosci.

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“…Baranovicova et al [ 57 ] showed that AUC > 0.91 for 10 metabolites, while only for the BCAAs, AUC = 0.733. Only for the combination of 10 amino acids, Han et al [ 58 ] showed that AUC = 0.91. Thus, individual amino acids have a low diagnostic value for the detection of breast cancer, while combinations of a number of amino acids with each other or with other metabolites provide a higher accuracy.…”

Section: Diagnostic Value Of Determination Of Amino Acids In Biologic...mentioning

confidence: 99%

“…Han et al [ 58 ] studied TNBC (tumor control) as well as the non-malignant pathologies of mammary glands. Four amino acids (Phe, Tyr, Ser, and Arg) were found to be more common in the triple-negative breast cancer (TNBC) group, while six amino acids (Pro, Trp, Val, Thr, Ala, and His) were more common in the benign group.…”

Section: Diagnostic Value Of Determination Of Amino Acids In Biologic...mentioning

confidence: 99%

The Role of Amino Acids in the Diagnosis, Risk Assessment, and Treatment of Breast Cancer: A Review

Bel’skaya,

Gundyrev,

Solomatin

2023

CIMB

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This review summarizes the role of amino acids in the diagnosis, risk assessment, imaging, and treatment of breast cancer. It was shown that the content of individual amino acids changes in breast cancer by an average of 10–15% compared with healthy controls. For some amino acids (Thr, Arg, Met, and Ser), an increase in concentration is more often observed in breast cancer, and for others, a decrease is observed (Asp, Pro, Trp, and His). The accuracy of diagnostics using individual amino acids is low and increases when a number of amino acids are combined with each other or with other metabolites. Gln/Glu, Asp, Arg, Leu/Ile, Lys, and Orn have the greatest significance in assessing the risk of breast cancer. The variability in the amino acid composition of biological fluids was shown to depend on the breast cancer phenotype, as well as the age, race, and menopausal status of patients. In general, the analysis of changes in the amino acid metabolism in breast cancer is a promising strategy not only for diagnosis, but also for developing new therapeutic agents, monitoring the treatment process, correcting complications after treatment, and evaluating survival rates.

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“…A number of studies have shown that amino acids used as potential biomarkers vary for different types of cancer, and changes in the concentration of amino acids in body fluids and tissues are important for diagnosing cancer, as well as choosing treatment tactics [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Salivary Amino Acid Levels in Cancer

2023

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This review analyzed 21 scientific papers on the determination of amino acids in various types of cancer in saliva. Most of the studies are on oral cancer (8/21), breast cancer (4/21), gastric cancer (3/21), lung cancer (2/21), glioblastoma (2/21) and one study on colorectal, pancreatic, thyroid and liver cancer. The amino acids alanine, valine, phenylalanine, leucine and isoleucine play a leading role in the diagnosis of cancer via the saliva. In an independent version, amino acids are rarely used; the authors combine either amino acids with each other or with other metabolites, which makes it possible to obtain high values of sensitivity and specificity. Nevertheless, a logical and complete substantiation of the changes in saliva occurring in cancer, including changes in salivary amino acid levels, has not yet been formed, which makes it important to continue research in this direction.

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Serum amino acids quantification by plasmonic colloidosome-coupled MALDI-TOF MS for triple-negative breast cancer diagnosis

Cited by 9 publications

References 34 publications

Metabolomics unveils the exacerbating role of arachidonic acid metabolism in atherosclerosis

Metabolomics unveils the exacerbating role of arachidonic acid metabolism in atherosclerosis

The Role of Amino Acids in the Diagnosis, Risk Assessment, and Treatment of Breast Cancer: A Review

Diagnostic Value of Salivary Amino Acid Levels in Cancer

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