Fluorimetric Study on the Interaction between Norfloxacin and Proflavine Hemisulphate

Abstract

The interaction between Norfloxacin (NF) and Proflavine hemisulphate (PF) was investigated by spectroscopic tools like UV-VIS absorption and Fluorescence spectroscopy. It was proved that fluorescence quenching of NF by PF is due to the formation of NF-PF complex which was supported by UV-VIS absorption study. The study of thermodynamic parameters suggested that the key interacting forces are hydrogen bond and van der Waal's interactions and the binding interaction was spontaneous. The distance r between NF and PF was obtained according to the Förster's theory of non-radiative energy transfer. The fluorescence quenching mechanism was applied to estimate PF directly from pharmaceutical samples.

Hetero-association of aromatic molecules in aqueous solution

“…Hence, the hetero-association of these types of molecules has been mainly performed by NMR spectroscopy, except for a few cases when reliable decomposition of UV/Vis was possible (e.g. [207]). …”

“…A number of systems have been studied by NMR under similar solution conditions (0.1 M ionic strength, pH~7.1): the hetero-association of phenanthridine (EB, PI, ethidium mono-and di-azides) [189,[208][209][210][211], acridine (AO, PF) [74,207] and xanthene [212] dyes with the antibiotics DAU, TPT and quinolones; the hetero-association of antitumor antibiotics DAU-NOV [213], DAU-AMD [214,215], DAU-NOR [168], DAU-NOG [167,191], NOV-AMD [214], NOV-NOG [167,191], NOR-NOG [168], NOG-AMD [167,191], DAU-TPT [189], antimalarial drugs [216] and the hetero-association of mutagens: AO-PF [217], EB-PI [218], PF-EB [75,76], dyes [219] and some other aromatic drug molecules [13,220]. These molecules have a planar 3-or 4-ring aromatic chromophore (except the 2-ring NOR molecule) and must potentially be characterised by greater π-stacking interactions than that provided by the derivatives of nucleic acid bases and xanthines.…”

“…This fact supports the conclusion that hetero-association is mainly driven by stacking interactions with the major contributions from hydrophobic and van der Waals interactions. In rare cases, the possibility of charge-transfer was inferred from fluorescence spectroscopy [207]. In some cases, the structures of the hetero-complexes appeared to be more compact compared to the homo-aggregates.…”

Evaluation of interparticle interaction between colloidal Ag nanoparticles coated with trisodium citrate and safranine by using FRET: Spectroscopic and mechanistic approach

“…Based on these data, we found R 0 = 2.674 nm and r = 2.807 nm which is less than 8 nm and 0.5 R 0 < r < 1.5 R 0 [22,23]. This clearly indicates that the energy transfer from AgNPs to safranine occurs with high efficiency.…”

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

“…Organic probes based on fluorescence quenching approach are widely used for detection and sensing of molecules of physicochemical , biological and environmental concern . Probe materials in the form of doped polymer film , microcrystal solution and colloidal dispersion at nanoscale have been found suitable for sensing applications.…”