Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach

Abstract

A derivação fotoquímica é proposta como abordagem para induzir fluorescência intensa (412/465 nm) da eritromicina. Parâmetros experimentais importantes como o tempo de irradiação com UV e tipo e concentração de ácido usado para tratar a solução de analito foram ajustadas. Limites de detecção e quantificação de 0,025e 0,085 µg mL -1 foram obtidos com resposta linear até 200 µg mL -1 . O procedimento é seletivo em relação a antibióticos aminoglicosídicos (canamicina, gentamicina e amicacina). O método foi testado em formulações farmacêuticas e em uma vacina contendo eritromicina como conservante, com recuperações do analito entre 98 e 105%.Photochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL -1 were achieved with linear range up to 200 µg mL -1 . The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%.

Green analytical methods for simultaneous determination of compounds having relatively disparate absorbance; application to antibiotic formulation of azithromycin and levofloxacin

“…In literature, perchloric and sulfuric acids have been added as reagents on certain macrolides such as erythromycin, spiramycin, troleandomycin and oleandomycin for some spectrofluorimetric and spectrophotometric methods. Perchloric and sulfuric acids help to create chromophores to determine some macrolides [ 13 , 14 , 15 , 16 ]. They promote breaking of large particles into smaller ones with greater quantum yield [ 16 ].…”

“…Perchloric and sulfuric acids help to create chromophores to determine some macrolides [ 13 , 14 , 15 , 16 ]. They promote breaking of large particles into smaller ones with greater quantum yield [ 16 ]. Perchloric acid (E0 = 1.75 V) is stronger than sulfuric acid (E0 = 1.44 V) and has a higher oxidative power.…”

Pharmaceuticals and Related Drugs

“…Many of the experimental procedures involve direct measurements of the analyte after relatively simple sample preparations steps, usually involving an extraction for cleanup, and others are based on more elaborate sample handling and chemical derivatization steps. A few examples of the many spectrophotometric assays appear in Table − . In the selection of these citations, particular emphasis was placed on chemiluminesence and fluorescence approaches and in selecting a cross-section of different types of compounds.…”

Enhanced electrochemical detection of erythromycin based on acetylene black nanoparticles

“…Therefore, the detection of erythromycin in honey samples is quite important. Up-to-now, several methods have been applied for the detection of erythromycin, such as liquid chromatography-mass spectrometry (LC-MS) [7,8], high-performance liquid chromatography (HPLC) with chemiluminescence detection [9], fluorimetric method [10], electrochemiluminescence [11], near infrared reflectance spectroscopy [12], and extraction-colorimetric method [13]. Erythromycin was also detected using electrochemical methods since it can be oxidized under proper conditions [14].…”