Fluorimetric Determination of L-3-Hydroxybutyrate Concentrations in the Serum of Normal and Aristolochic Acid-Treated Mice

Establishment of a two-dimensional chiral HPLC system for the simultaneous detection of lactate and 3-hydroxybutyrate enantiomers in human clinical samples

“…A previous study showed that in kidney-injured rats, the concentration of L-3HB had a rising trend when it was examined alone. However, when compared with D-3HB, the proportion of L-3HB in total 3HB (%L-3HB) declined [34]. Currently, the in vivo functions and effects of 3HB enantiomers are unclear.…”

Construction of a light‐emitting diode fluorescence detector for high‐performance liquid chromatography and its application to fluorometric determination of l‐3‐hydroxybutyrate

“…We performed a derivatization reaction of standard or rat serum containing 3‐HB with a fluorescence reagent, NBD‐PZ, by modifying our previously published method (Chen et al , ; Tsai et al , , ). To determine the total ( d + l )‐3HB derivatives in rat serum, 20 μL of the filtrate from 0.45 µm Millipore filter was injected into the HPLC equipped with a Biosil ODS column (150 × 4.6 mm i.d.…”

“…Furthermore, d ‐3HB led to a reduction in the utilization of glucose, gradually recovering dose‐dependently following supplementation with additional l ‐3HB (Tsai et al , ). In addition, the concentration of l ‐3HB in the serum of aristolochic acid‐treated mice was significantly elevated, compared with that of normal mice, suggesting an alteration in l ‐3HB associated with particular pathological conditions (Chen et al , ). Although the improvement in HPLC method has led to some understanding of the characteristics and functions of l ‐3HB, many questions remain unanswered and continuous efforts in both analytical method and pathophysiological study are warranted.…”

“…We previously developed a highly sensitive column‐switching HPLC method using fluorescence excitation for the verification, identification and quantification of l ‐3HB in mammals (Tsai et al , , ). Using our HPLC method, the concentrations of l ‐3HB in the serum, liver, heart and kidney of normal rats, the heart of diabetic rats, and the serum of normal and aristolochic acid‐treated mice can be measured (Chen et al , ; Tsai et al , , ). Among all of the examined tissues, the heart homogenate contained the highest concentration of l ‐3HB, and the implication of this finding is still unknowm.…”