Development of a selective three-dimensional HPLC system for enantiomer discriminated analysis of lactate and 3-hydroxybutyrate in human plasma and urine

Hsieh, Chin Ling; Akita, Takeyuki; Mita, Masashi; Ide, Tomomi; Lee, Jen Ai; Hamase, Kenji

doi:10.1016/j.jpba.2020.113871

Cited by 8 publications

(10 citation statements)

References 28 publications

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“…Several LC approaches with chiral derivatization reagents [25][26][27][28][29] or chiral stationary phases [14,36,[39][40][41] have already been utilized for the enantioselective separations of LA, 2HB, 3HB and MA. With regard to the chiral derivatization methods, benzyl 5-(2-aminoethyl)-3-methyl-4-oxoimidazolidine-1-carboxylate was used to derivatize LA, 2HB, 3HB and MA, and the diastereomeric derivatives were separated on a reversed-phase column [25].…”

Section: Enantioselective Separations Of La 2hb 3hb and Ma As Their N...mentioning

confidence: 99%

“…Until now, to the best of our knowledge, no method has been developed to simultaneously analyze these hydroxy acid enantiomers in real biological samples by only one injection. However, the methods using an enzymatic reaction [19,20], capillary electrophoresis [21,22], gas chromatography [23,24], high-performance liquid chromatography (HPLC) with various detectors [25][26][27][28][29][30][31][32][33][34] and multi-dimensional HPLC methods [5,14,[35][36][37][38][39][40][41][42] have been reported to separately analyze these chiral hydroxy acids. Among them, HPLC methods (including column-switching/ multi-dimensional systems) are widely used for the chiral analysis of the hydroxy acids.…”

Section: Introductionmentioning

confidence: 99%

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Two-Dimensional Chiral HPLC Analysis of Lactate, Hydroxybutyrates and Malate in Human Plasma

et al. 2022

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For the simultaneous determination of lactate (LA), 2-hydroxybutyrate (2HB), 3-hydroxybutyrate (3HB) and malate (MA) enantiomers, a new separation technique in a two-dimensional high-performance liquid chromatography (2D-HPLC) system following the fluorescence derivatization with 4-nitro-7-piperazino-2,1,3-benzoxadiazole has been developed. The 2D-HPLC was composed of the reversed-phase (1D) and enantioselective (2D) separations. In the first dimension, the target hydroxy acids were separated as their D plus L mixtures on a Singularity RP18 column (1.0 mm i.d. x 250 mm), and the enantiomers were separated in the second dimension using a polysaccharide-based chiral stationary phase, Chiralpak IG (2.0 mm i.d. x 250 mm). By using the Chiralpak IG column, the LA, 2HB, 3HB and MA enantiomers were separated with resolution values of 3.47, 3.63, 6.81 and 3.72, respectively. The developed 2D-HPLC system was applied to human plasma, and trace levels of D-LA and L-3HB in addition to their major antipodes were determined. The acquired %D of LA (the percentage of D-LA over total LA) and %L of 3HB (the percentage of L-3HB over total 3HB) were 1.0 and 2.7, respectively. For 2HB, only the L-form was detected, while the MA enantiomers were not found in the human plasma.

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Section: Enantioselective Separations Of La 2hb 3hb and Ma As Their N...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Two-Dimensional Chiral HPLC Analysis of Lactate, Hydroxybutyrates and Malate in Human Plasma

et al. 2022

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“…The LA enantiomers were determined by the highly specific 3D-HPLC system according to a previous study with slight modifications [30]. The flow diagram of the 3D-HPLC system is shown in Fig.…”

Section: D-hplc System For the Determination Of La Enantiomersmentioning

confidence: 99%

“…Although these methods are useful, numerous peaks still appeared around the peak region of typical target compounds in the chromatogram and sometimes affected the trace analysis of the minor form of the enantiomers, thus a method with a higher selectivity is required. As a highly selective method, the three-dimensional HPLC (3D-HPLC) equipped with the reversed-phase, mixed-mode and enantioselective separations is one of the most useful methods, and applicable to various complicated matrices including biological and clinical samples [29,30]. In the present study, a 3D-HPLC system was designed/developed for the determination of LA enantiomers in various fermentation food/beverage samples including vinegars.…”

Section: Introductionmentioning

confidence: 99%

Chiral Analysis of Lactate in Various Food Samples Including Japanese Traditional Amber Rice Vinegar and the Developmental Changes During Fermentation Processes

et al. 2021

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Enantioselective analysis of lactate (LA) in various fermented food/beverage samples including the Japanese traditional amber rice vinegars was performed using a newly designed three-dimensional high-performance liquid chromatographic (3D-HPLC) system. The enantiomers of LA are reported to be possible biomarkers for some diseases such as diabetic mellitus. To evaluate the diagnostic value, the dietary intake should be taken into consideration. Therefore, clarifying the presence of LA enantiomers in our environment is a matter of interest. In the present study, LA enantiomers in a variety of fermented food/beverage samples were determined after the pre-column derivatization with 4-nitro-7-piperazino-2,1,3-benzoxadiazole. Among the fermented products tested, high concentrations of LA enantiomers were observed in a Japanese traditional amber rice vinegar (sample SK, 13.1 and 22.8 μmol/mL for D-LA and L-LA, respectively). The developmental changes of the LA enantiomers during the fermentation/aging processes of the Japanese traditional amber rice vinegar were also studied. The concentrations of D-and L-LA drastically increased in the early stage of the fermentation and attained to the highest concentrations after 1 month, then gradually decreased thereafter. The present results help to illuminate the benefits of Japanese traditional vinegars in the health and food sciences.

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“…There are many methods for the determination of lactate such as polarimetry [ 5 ], gas chromatography (GC) [ 6 ], high performance liquid chromatography (HPLC) [ 7 ], and nuclear magnetic resonance (NMR) [ 8 ]. However, these methods are expensive and require complicated sample preparation and trained personnel.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Flexible Arrayed Lactate Biosensor Based on Copper Doped Zinc Oxide Films Modified by Iron–Platinum Nanoparticles

Nien

Kang

et al. 2021

Polymers

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Potentiometric biosensors based on flexible arrayed silver paste electrode and copper-doped zinc oxide sensing film modified by iron-platinum nanoparticles (FePt NPs) are designed and manufactured to detect lactate in human. The sensing film is made of copper-doped zinc oxide (CZO) by a radio frequency (RF) sputtering system, and then modified by iron-platinum nanoparticles (FePt NPs). The surface morphology of copper-doped zinc oxide (CZO) is analyzed by scanning electron microscope (SEM). FePt NPs are analyzed by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The average sensitivity, response time, and interference effect of the lactate biosensors are analyzed by voltage-time (V-T) measurement system. The electrochemical impedance is analyzed by electrochemical impedance spectroscopy (EIS). The average sensitivity and linearity over the concentration range 0.2 mM–5 mM are 25.32 mV/mM and 0.977 mV/mM, respectively. The response time of the lactate biosensor is 16 s, with excellent selectivity.

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Development of a selective three-dimensional HPLC system for enantiomer discriminated analysis of lactate and 3-hydroxybutyrate in human plasma and urine

Cited by 8 publications

References 28 publications

Two-Dimensional Chiral HPLC Analysis of Lactate, Hydroxybutyrates and Malate in Human Plasma

Two-Dimensional Chiral HPLC Analysis of Lactate, Hydroxybutyrates and Malate in Human Plasma

Chiral Analysis of Lactate in Various Food Samples Including Japanese Traditional Amber Rice Vinegar and the Developmental Changes During Fermentation Processes

Investigation of Flexible Arrayed Lactate Biosensor Based on Copper Doped Zinc Oxide Films Modified by Iron–Platinum Nanoparticles

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