Fluorimetric Determination of Gentamicin in Dosage Forms and Biological Fluids Through Derivatization with 4-Chloro-7-Nitrobenzo-2-Oxa-1,3-Diazole (NBD-Cl)

Al-Majed, Abdulhakeem A.; Belal, F.; Abounassif, Mohammad A.; Khalil, Nasr Y.

doi:10.1007/s00604-002-0920-x

Cited by 27 publications

(14 citation statements)

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“…In recentr eports. NBD-Clw as further used as a chromogenic reagent for the determinationofsome primary and secondaryamines [12][13][14][15][16]. In the present study, ISX was foundt or eactw ith NBD-Cl in borate buffer of pH 7.8 producing ayellow color with maximum absorbance at 395 nm.…”

Section: Resultsmentioning

confidence: 93%

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Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

2006

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As imple and sensitivek inetic spectrophotometric method was developedf or the determinationo fi soxsuprine in pharmaceutical preparations.T he method is basedupon akinetic investigationofthe coupling reaction between isoxsuprine HCl and4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8f or af ixedt ime of 30 min. The absorbance of the yellow coloured product was measured at 395 nm. The absorbance-concentrationp lot was rectilinear over the rangeo f2 -20 µg mL -1 (r= 0.9994) with minimum detectability of 0.6 µg mL -1 (1.75 x10 -6 M). The different experimental parameters affecting the development and stability of the colourw ere carefully studied and optimized. The determination of isoxsuprine by the fixed-concentration and rate-constantm ethods is also feasible with the calibratione quations obtained, but the fixed time methodh as been found to be more applicable.T he proposed methodw as further applied to the determination of the drug in formulations. The results obtained were in good agreementw ith thoseo btained usingar eferencem ethod. The reaction pathway wasproposed. KeywordsKineticd etermination,i soxsuprine HCl, (NBD-Cl),p harmaceutical preparations.Scientia Pharmaceutica (Sci. Pharm.) 74, 99-119

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Section: Resultsmentioning

confidence: 93%

“…The latteri sau seful derivatizing agent for primary and secondary amines, thiol, etc. Several pharmaceutical compounds have been determined through this approach [11][12][13][14][15][16].…”

mentioning

confidence: 99%

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

2006

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“…The analysis of different compounds or drugs either in pharmaceuticals or in biological samples was performed after derivatization with NBD-Cl followed by measuring the resulted product by means of spectrophotometry [46][47][48][49], Spectrofluorimetry [50][51][52], HPLC as in determination aliphatic amines [53].…”

Section: Resultsmentioning

confidence: 99%

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

et al. 2013

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KEYWORDSSeveral simple, sensitive, accurate and inexpensive spectrophotometric and spectrofluorimetric methods were developed for the determination of propafenone HCl and diltiazem HCl using 4-chloro-7-nitrobenzofurazan (NBD-Cl) accompanied with kinetic study, either in pure form or in pharmaceutical preparations. In this work, the cited drugs react with (NBD-Cl) in presence of borate buffer of pH = 7.6 at a fixed time of 30 minutes on thermostated water bath at (75-80 °C). The absorbance was measured using spectrophotometric technique at 489 and 481 nm for propafenone HCl and diltiazem HCl, respectively, or by using spectrofluorimetric technique after dilution at the specific wavelength of excitation and emission.The calibration curves were linear in the range of 4-44, 16-96 µg/mL when using spectrophotometric method, and 0.4-3.6, 1.6-8.8 µg/mL when spectrofluorimetric method was applied for propafenone HCl and diltiazem HCl, respectively. The limit of quantitation and the limit of detection were also calculated. The methods were applied successfully to commercial dosage form and can be further applied for their determination on a large scale in quality control laboratories. The obtained results statistically agreed with those obtained by reference methods. The determination of the studied drugs by the fixed concentration and rate constant methods is feasible with the calibration equations obtained, but the fixed time method proves to be more applicable.Diltiazem HCl Propafenone HCl Spectroflourimetric Spectrophotometric Kinetic determination 4-Chloro-7-nitrobenzofurazan

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“…No intuito de obter um sinal fluorescente, a formação de complexos entre o fármaco e outras espécies tem sido bastante explorada por muitos grupos de pesquisa 15,74,78,[94][95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110]117,132 (Tabela 3). Muitos artigos têm demonstrado que a inclusão do fármaco dentro da cavidade de ciclodextrinas pode induzir a emissão de várias moléculas.…”

Section: Complexos Fluorescentes De Fármacosunclassified

“…137 Adicionalmente, pesquisas demonstraram que a intensidade da fluorescência em meios micelares de tensoativos não-iônicos é grandemente amplificada, permitindo a determinação de alguns fármacos em matrizes onde se encontram a níveis de traços, como em alimentos. 5 Complexos formados entre fármacos e compostos orgânicos como 4-cloro-7-nitrobenzo-2-oxa-1,3-diazol e 4-cloro-7-nitrobenzofurazan para determinar gentamicina 109 e nortriptilina, 110 respectivamente, permitiram a quantificação destes fármacos a níveis de µg mL -1 .…”

Section: Complexos Fluorescentes De Fármacosunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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Recebido em 20/10/06; aceito em 30/11/07; publicado na web em 4/9/08 APPLICATION AND ADVANCES IN THE LUMINESCENCE SPECTROSCOPY IN PHARMACEUTICAL ANALYSES. This paper provides a review on the latest advances and applications of the luminescence spectroscopy for the development of pharmaceuticals analyses methods, basically based on the photo-and chemiluminescence. The different forms of the drugs determination on pharmaceuticals through the fluorescence and chemiluminescence are discussed. The analyses include the drugs native fluorescence (liquid and solid-phases); the fluorescence from the oxidizing or reducing forms of the drug; the fluorescence from the chemical derivatization and their photochemistry and hydrolysis reactions. The quenching of luminescence and chemiluminescence generation for the pharmaceutical quantification are also shown. Finally, the trends and future perspectives of the luminescence spectroscopy in the field of the pharmaceutical research are discussed.Keywords: luminescence spectroscopy; drugs; cosmetics analysis; introdução Devido ao progresso contínuo em técnicas e instrumentos, a indústria farmacêutica busca o emprego de metodologias que ofereçam maior sensibilidade, seletividade, robustez, precisão, exatidão e rapidez. Neste sentido, um grande número de equipamentos de cromatografia líquida de alta eficiência (CLAE) com diversos tipos de detecção domina este ramo industrial. Contudo, o advento da sofisticação de instrumentação automatizada de espectrômetros de luminescência poderá resultar em um substancial aumento de aplicações desta técnica instrumental na indústria farmacêutica, principalmente, no que se refere à análise diretamente na matriz do medicamento.A espectroscopia de luminescência é uma ferramenta analítica extremamente sensível e tem sido amplamente aplicada na resolução de problemas que requerem baixos limites de detecção, podendo ser facilmente encontrados na literatura muitos trabalhos mostrando análises ao nível de traços.1-6 A sensibilidade inerente a esta técnica é consideravelmente maior em comparação a outras metodologias, entre elas a espectrofotometria UV/visível, apresentando limites de detecção de até três ordens de grandeza menores, os quais normalmente se encontram na faixa de ng mL -1 , sendo conseqüência do baixo sinal de fundo que as medidas fluorescentes apresentam. Diferentemente do que ocorre no fenômeno da luminescência, nas medidas de absorbância o sinal está associado à atenuação da intensidade de um feixe de radiação por uma espécie absorvente. Nos casos onde a quantidade da espécie é pequena, esta atenuação é menor, tornando assim a detecção da espécie difícil. Outra característica importante da luminescência diz respeito à seletividade, porque espécies com rendimentos quânticos de fluorescência apreciáveis são menos comuns do que substâncias cuja fluorescência não pode ser detectada. Desta forma, moléculas fluorescentes apresentam comprimento de onda característico de excitação e/ou emissão. Adicionalmente, o comprimento de onda de excitação d...

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Fluorimetric Determination of Gentamicin in Dosage Forms and Biological Fluids Through Derivatization with 4-Chloro-7-Nitrobenzo-2-Oxa-1,3-Diazole (NBD-Cl)

Cited by 27 publications

References 0 publications

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

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