Fluorimetric determination of alkaline phosphatase activity in human serum by use of a flow-through biosensor

Fluorometric Determination of Alkaline Phosphatase Activity in Food Using Magnetoliposomes as On-flow Microcontainer Devices

“…As can be seen, the dynamic range of the calibration graph in the presence of MLs is 6.4 × 10 –3 –0.250 U L –1 ALP, and the limit of detection (LOD) is 1.9 × 10 –3 U L –1 , which is 58 times lower than that obtained in the absence of MLs (0.11 U L –1 ). Also, the LOD is 26 times lower that that reached by retaining the product of the enzymatic reaction in a resin placed in the flow cell (0.05 U L –1 ) . Table also shows the repeatability ( n = 7, r = 3) and reproducibility ( n = 5, r = 3) data, expressed as RSD%, obtained in the presence and absence of liposomes.…”

“…Statistical analyses were performed with Statgraphics Plus 5.1 software (Statistical Graphics Corp.). The paired t test was used to compare the results obtained for the analysis of milk samples using both the new method and the previously reported method …”

“…The new method for ALP determination has been applied to the analysis of raw and commercial milk samples. The results have been compared with those obtained using a method based on the same enzymatic reaction, but involving the retention of the reaction product on a resin placed in the cell flow . Although several methods have been described for the determination of ALP activity in milk samples, − the above-mentioned method was chosen because it also involves the use of MUP and a flow system, which enables an effective assessment of the MLs on the system.…”

“…The results have been compared with those obtained using a method based on the same enzymatic reaction, but involving the retention of the reaction product on a resin placed in the cell flow. 23 Although several methods have been described for the determination of ALP activity in milk samples, 24−26 the above-mentioned method was chosen because it also involves the use of MUP and a flow system, which enables an effective assessment of the MLs on the system. The usefulness of the method has been also checked by its application to study the milk pasteurization process by monitoring the degradation of ALP.…”

Synthesis of 2‐(1H‐Benzo[d]imidazol‐2‐yl)phenyl Phosphate as a Fluorescent Probe for Determination of ALP

“…5 Therefore, the application of fluorimetric approach, especially the fluorogenic substrates such as phosphatases which are widely distributed enzymes in many types of cells, has been proposed for an assessment of intracellular phosphatase activity. 6 Meanwhile, it is noted that only a few of these fluorogenic substrates, such as salicylic acid phosphate, 7 β-naphthyl phosphate 8 and 2-carboxy-1-naphthyl phosphate 9 along with their derivatives have been reported. Characterized with excited-state intramolecular proton transfer (ESIPT) property, 2-(2-hydroxyphenyl) benzimidazole(HPBI) and its derivatives become a series of important fluorescent compounds.…”