Synthesis of 2‐(1H‐Benzo[d]imidazol‐2‐yl)phenyl Phosphate as a Fluorescent Probe for Determination of ALP

Abstract

A convenient method to synthesize 2-(1H-benzo [d]imidazol-2-yl)phenyl phosphate (HBIPP), a substrate for the fluorescent determination of alkaline phosphatase (ALP) activity, via the reaction of salicylaldehyde and o-phenylenediamine in the presence of manganese(III) acetate was realized. The structure was confirmed by IR, 1 H NMR, EI-MS and elemental analysis studies while the corresponding photo-physical properties of the enzyme reaction product were characterized by ultraviolet absorption and fluorescence spectra in methanol. Preliminary tests reveal that the amount of the enzyme reaction product, 2-(2-hydroxyphenyl) benzimidazole (HPBI), is proportional to ALP activity in the range of 0.01-5 U/L with the detection limit of 8.5 mU/L, which can be applied to assess ALP in biomedical research.

Synthesis, Thermoanalysis, and Thermal Kinetic Thermogravimetric Analysis of Transition Metal Co(II), Ni(II), Cu(II), and Zn(II) Complexes with 2-(2-Hydroxyphenyl)benzimidazole (HL)

“…The fluorescent compound 2-(2-hydroxyphenyl)benzimidazole (HPBI) (Figure ) is useful as a laser dye, high energy radiation detector, molecular energy storage system, and fluorescent probe. − The excited state intramolecular proton transfer mechanism about it and its analogues have been extensively studied, − which showed a high Stokes shift and a great thermal and photophysical stability.…”