Fluoride mediates apoptosis in osteosarcoma UMR 106 and its cytotoxicity depends on the pH

Hirano, Seishiro; Ando, Masayuki

doi:10.1007/s002040050468

Cited by 30 publications

(13 citation statements)

References 33 publications

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“…However, the cytotoxicity of fluoride is pH dependent [47,48] and previous studies have shown that fluoride is cytotoxic in acidic pH but has minimum cytotoxic effect in neutral pH [47,48]. Therefore, the ability of S-PRG cement to maintain the pH of the soaking water unchanged throughout the soaking period might be Further studies are required to investigate other variables of S-PRG cement that are related to the setting process such as dimensional stability, solubility and expansion.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

et al. 2016

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Materials and methods:The antibacterial effect of S-PRG, MTA and IRM cements were tested against Porphyromonas gingivalis and Enterococcus faecalis after one and three-days of aging of the cements. Set cements were immersed in distilled water for 4 hours to 28 days and ion releasing ability was evaluated. Initial and final setting times of all cements were evaluated using Gilmore needles. The push-out bond strength between radicular dentin and all cements was tested at different levels of the roots.Results: S-PRG and IRM cements but not MTA cement demonstrated significant antibacterial effect against Porphyromonas gingivalis. All types of cements exhibited significant antibacterial effect against Enterococcus faecalis without being able to eliminate the bacterium. S-PRG cement provided continuous release of fluoride, strontium, boron, sodium, aluminum and zinc throughout all tested time points. Both initial and final setting times were significantly shorter for S-PRG and IRM cements in comparison to MTA. The push-out bond strength was significantly lower for S-PRG cement in comparison to MTA and IRM at coronal and middle levels of the roots. Conclusions:S-PRG cement demonstrated significant antibacterial effects against endodontic pathogens, multiple ion releasing ability, relatively short setting time and low bonding strength.Clinical relevance: S-PRG cement can be used as a one-visit root repair material with promising antibacterial properties and ion releasing capacity.

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Section: Discussionmentioning

confidence: 99%

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

et al. 2016

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“…On the other hand, few reports could not find a link between drinking water fluoride and incidence of bone cancers [14,15]. In vitro studies have demonstrated mitogenic effect of fluoride on bones [3].…”

Section: Discussionmentioning

confidence: 99%

“…Fluoride is also recognized as stimulator of bone formation via osteoblast proliferation. Although an excess intake of fluoride has been reported to cause skeletal fluorosis [3], very little is known about the mechanism of adverse effects of fluoride in bone.…”

Section: Introductionmentioning

confidence: 99%

Serum Fluoride and Sialic Acid Levels in Osteosarcoma

Sandhu

Lal

Kundu

et al. 2009

Biol Trace Elem Res

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Osteosarcoma is a rare malignant bone tumor most commonly occurring in children and young adults presenting with painful swelling. Various etiological factors for osteosarcoma are ionizing radiation, family history of bone disorders and cancer, chemicals (fluoride, beryllium, and vinyl chloride), and viruses. Status of fluoride levels in serum of osteosarcoma is still not clear. Recent reports have indicated that there is a link between fluoride exposure and osteosarcoma. Glycoproteins and glycosaminoglycans are an integral part of bone and prolonged exposure to fluoride for long duration has been shown to cause degradation of collagen and ground substance in bones. The present study was planned to analyze serum fluoride, sialic acid, calcium, phosphorus, and alkaline phosphatase levels in 25 patients of osteosarcoma and age- and sex-matched subjects with bone-forming tumours other than osteosarcoma and musculo-skeletal pain (controls, 25 each). Fluoride levels were analyzed by ISE and sialic acid was analyzed by Warren's method. Mean serum fluoride concentration was found to be significantly higher in patients with osteosarcoma as compared to the other two groups. The mean value of flouride in patients with other bone-forming tumors was approximately 50% of the group of osteosarcoma; however, it was significantly higher when compared with patients of group I. Serum sialic acid concentration was found to be significantly raised in patients with osteosarcoma as well as in the group with other bone-forming tumors as compared to the group of controls. There was, however, no significant difference in the group of patients of osteosarcoma when compared with group of patients with other bone-forming tumors. These results showing higher level of fluoride with osteosarcoma compared to others suggesting a role of fluoride in the disease.

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“…It was documented that NaF at 5 mM decreased the viability of the osteosarcoma cell line UMR 106 to approximately 50% of that of controls following an 8-h exposure [16]. Additionally, rat bone marrow cells treated with 10 mM NaF revealed a statistically significant increased proportion of nonviable cells (62-65%) after exposure times of 12 h, 24 h, or 36 h [19].…”

Section: Discussionmentioning

confidence: 99%

Chemical-biological interactions of NaF with three different cell lines and the caries pathogen Streptococcus sobrinus

et al. 2002

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Fluoride is used in dentistry as a prophylactic agent to reduce caries rates due to the demineralization/remineralization effect and its influence on the metabolism of cariogenic bacteria. The purpose of this study was to evaluate the cytotoxic effects of sodium fluoride (NaF) on three different cell lines and the antibacterial potency on Streptococcus sobrinus. Cell lines were treated with various concentrations of NaF ranging from 0.039 mM to 10 mM for 24 h. For microbial assays, concentrations of NaF between 0.03 mM and 10 mM were added to liquid cultures of bacteria. Our results showed that immortalized human keratinocytes (HaCaT) and human osteogenic sarcoma cells (SAOS-2) were similarly affected by concentrations up to 2.5 mM. However, cell growth of HaCaT was slightly more inhibited at 2.5 mM of NaF than SAOS-2. At concentrations between 0.62 mM and 10 mM, 3T3 mouse fibroblast cells reacted more sensitively than HaCaT and SAOS-2 to NaF. The 3T3 cells did not survive in the presence of 10 mM NaF. NaF caused no significant effect on all tested cells at concentrations of < or = 0.31 mM. NaF at 0.039 mM and 0.06 mM did not affect growth of S. sobrinus. At concentrations of 0.125 mM and 0.5 mM, growth was slightly reduced. The proliferation of S. sobrinus significantly decreased at 1 mM and 2 mM NaF. S. sobrinus survived at 4 mM, revealing a delayed log phase with a decreased proliferation. No viable S. sobrinus cells were detected at concentrations of > or = 8 mM NaF. Data analysis revealed that overall treatment effects were highly significant (P<0.05, analysis of variance, Tukey's difference test). This study indicates that cytotoxic effects due to NaF significantly vary in dependence upon the applied cell line. The toxicity of NaF approached 50% (TC50) at concentrations of 6 mM for HaCaT, 2.3 mM for 3T3 cells, and 7.5 mM for SAOS-2. Additionally, NaF revealed antimicrobial effects only at concentrations that are significantly higher than oral fluoride concentrations.

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Fluoride mediates apoptosis in osteosarcoma UMR 106 and its cytotoxicity depends on the pH

Cited by 30 publications

References 33 publications

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers

Serum Fluoride and Sialic Acid Levels in Osteosarcoma

Chemical-biological interactions of NaF with three different cell lines and the caries pathogen Streptococcus sobrinus

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