Fluoride-induced oxidative stress and apoptosis are involved in the reducing of oocytes development potential in mice

Wang, Hongwei; Zhao, Wenpeng; Liu, Jing; Tan, Panpan; Zhang, Cai

doi:10.1016/j.chemosphere.2017.08.068

Cited by 60 publications

(17 citation statements)

References 51 publications

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“…GSH level is a critical factor known to inﬂuence oocyte quality. It has been reported that ROS accumulation accelerates the aging process in oocytes and influences their subsequent fertilization ability [37, 38]. Analysis of ROS and GSH levels revealed that aged oocytes in the LAM-treated group had significantly lower levels of ROS and higher levels of GSH, which resulted in the reduction of oxidative stress and an improvement in oocyte quality compared with the control group.…”

Section: Discussionmentioning

confidence: 95%

Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Yao

Jiang

Liang

et al. 2018

Journal of Reproduction and Development

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Laminarin (LAM) is a β-glucan oligomer known to possess biological activities such as anticancer and antioxidant effects. This study explored the influence of LAM supplementation on in vitro aged porcine oocytes and the underlying mechanisms behind this influence. We found that LAM delayed the aging process and improved the quality of aged oocytes. LAM supplementation enhanced the subsequent developmental competence of aged oocytes during the in vitro aging process. The blastocyst formation rate was significantly increased in aged oocytes treated with 20 µg/ml LAM compared to non-treated aged oocytes (45.3% vs. 28.7%, P < 0.01). The mRNA levels of apoptosis-related genes, B cell lymphoma-2-associated X protein (Bax) and Caspase-3, were signiﬁcantly lower in blastocysts derived from the LAM-treated aged oocytes during the in vitro aging process. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased in aged oocytes following LAM treatment. Mitochondrial membrane potential was increased, and the activities of caspase-3 and cathepsin B were significantly reduced in the LAM-treated aged oocytes compared with the non-treated aged oocytes. Taken together, these results suggest that LAM is beneficial for delaying the aging process in porcine oocytes.

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Section: Discussionmentioning

confidence: 95%

Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Yao

Jiang

Liang

et al. 2018

Journal of Reproduction and Development

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“…Gonadal retardation was observed in cuttlefish (SJE), and the GI SJE was lower than GI SJP . In a previous report, Wang et al () indicated that excessive fluoride intake can reduce oocyte developmental potential, indicating that removal of the exoskeleton or lowing the fluoride content would have to be considered if Atlantic krill were to be used as feed for cuttlefish in the future.…”

Section: Discussionmentioning

confidence: 99%

Raw frozen Antarctic krill (Euphausia superba) as an alternative feed source for cuttlefishSepiella japonicain artificial breeding systems

Shi

Ping

et al. 2020

Aquac Res

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The study aims to evaluate the feasibility of completely replacing raw frozen shrimp Palaemon gravieri diets with raw frozen Antarctic krill (Euphausia superba) in the diets of cuttlefish Sepiella japonica. To address the knowledge gap, we conducted a 60‐day feeding trial. At the end of the experiment (day 60), the cuttlefish Sepiella japonica eating Palaemon gravieri (SJP) grew significantly faster than those eating Euphausia superba (SJE), with the specific growth rate (SGR)SJP (7.92%) > (SGR)SJE (7.09%). Approximately 33.3% and 20.0% mortality was observed in the SJE and SJP, during the course of the experiment respectively. Some important fatty acids (i.e. n‐3 and n‐6 PUFAs) were elevated in SJE with respect to SJP. Replacement of Antarctic krill increased the diversity of the gut microbiome composition in the SJE group. Fluoride accumulated in the ink sac and cuttlebone of cuttlefish in SJE. Overall, these findings imply that PUFA‐rich Antarctic krill could replace P. gravieri shrimp for feeding cuttlefish S. japonica.

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“…However, spleen is one of the most susceptible immune organs to fluoride because of the accumulation of fluoride ions in its cells (Krishnamoorthy et al, 2015). Studies indicated that excessive fluoride ingestion induced mitochondrial damage and cellular apoptosis through larger accumulation of fluoride ions in cells (Panneerselvam et al, 2015; Sarkar et al, 2014, Wang et al, 2017b). In the present study, the structure of pathomorphological and subcellular organelles in the spleen was seriously damaged after fluoride treatment.…”

Section: Discussionmentioning

confidence: 99%

“…DNA damage assay was performed through single-cell gel electrophoresis (SCGE), as reported in our previous studies (Wang et al, 2017b). The grades of DNA damage were evaluated in accordance with previously described methods (Wang et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

“…Fluoride is an extremely active element in the environment. It exists in water, air, food, and soil and enters the body primarily through drinking water and food consumption (Wang et al, 2017a, 2017b; Zhou et al, 2015). According to World Health Organization, the maximum level of fluoride intake in drinking water is 1 – 1.5 mg/L to ensure its beneficial effects for adults (Mohammadi et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Induction of pathological changes and impaired expression of cytokines in developing female rat spleen after chronic excess fluoride exposure

et al. 2018

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This study was designed to investigate the effects of excessive fluoride on spleen toxicity. Twenty-four healthy female rats were randomly divided into two groups, each of 12 rats. Each group of female rats was given a control diet and either F− = 0 mg/L or an excessive F− = 150 mg/L in the drinking water for 120 days. The histomorphological and ultrastructural changes in their splenic tissues were observed under light and transmission electron microscopes. DNA damage and splenocyte apoptosis were examined using the micronucleus (MN) assay, single-cell gel electrophoresis (SCGE), and flow cytometry. The expression levels of cytokines, including interleukin (IL)-1β, IL-2, IL-6, and tumor necrosis factor (TNF)-α, were determined through immunohistochemistry and Western-blot analysis. Results demonstrated that the histomorphological characteristics and ultrastructure of the splenic tissues were affected by excessive fluoride. Nuclear dying, nuclear membrane dissolution, mitochondrial vacuolation, and endoplasmic reticulum dilation were observed. SCGE and MN assays showed that the nuclear DNA of splenocytes was damaged by fluoride treatment, and splenocyte apoptosis was exacerbated in the fluoride group. With damage to the splenocyte structure and DNA, the protein expression levels of IL-1β, IL-2, IL-6, and TNF-α were significantly downregulated by exposure to fluoride. Excessive fluoride ingestion caused splenic pathological damage and abnormal cytokine expression in female rats.

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Fluoride-induced oxidative stress and apoptosis are involved in the reducing of oocytes development potential in mice

Cited by 60 publications

References 51 publications

Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Raw frozen Antarctic krill (Euphausia superba) as an alternative feed source for cuttlefishSepiella japonicain artificial breeding systems

Induction of pathological changes and impaired expression of cytokines in developing female rat spleen after chronic excess fluoride exposure

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