Fluoreszenzanalytische Untersuchungen an Pflanzen

Klein, Gustav; Linser, H.

doi:10.1007/bf01844772

Cited by 22 publications

(7 citation statements)

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“…KOH solution or moist Ag20 it underwent a hydramine fission with formation of trimethylamine. Klein & Zeiler (1930) and Klein & Linser (1932) describe a method of estimating choline by decomposition with boiling concentrated alkali and distillation and.titration of the trimethylamine formed. The method involves treatment of the plant extract for 3 hr.…”

Section: Quantitative Etinmation Of Cholinementioning

confidence: 99%

“…The Estimation of Free Choline in Plants BY H. E. STREET, A. E. KENYON AND G. M. WATSON Univer8ity College, Nottingham (Received 1 July 1946) Choline, being a constituent of lecithin, is probably present in every living cell. Schulze & Trier (1912b) and Klein & Zeller (1930) have examined more than 100 plants of widely different systematic origin and, with the exception of the three lichens examined, were able to detect free choline in all cases.…”

mentioning

confidence: 94%

“…The significance of choline in the methylation processes of higher plants has not hitherto been investigated. Klein & Linser (1932, 1933 have opened up the subject of choline metabolism in higher plants by their study of variations of lecithin and choline contents during germination of various seeds.…”

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confidence: 99%

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The estimation of free chloine in plants

Street

Kenyon

Watson

1946

Biochemical Journal

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Section: Quantitative Etinmation Of Cholinementioning

confidence: 99%

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confidence: 94%

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The estimation of free chloine in plants

Street

Kenyon

Watson

1946

Biochemical Journal

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“…However, in view of the countless autofluorescent substances in plants (40,42), even the recording of a typical emission spectrum for lignin (31) cannot be regarded as a specific proof for the presence of lignin.…”

Section: Evidence For the Participation Of Lignification In Resistancmentioning

confidence: 99%

“…The most convenient way of determining lignin is provided by a range of histochemical staining reactions (37): A positive outcome of the phloroglucinol/HCl or the chlorine/sulfite test, supposed to be the most specific stains for lignin (38,39), point to the participation of lignin in the investigated resistance phenomena (23)(24)(25)(28)(29)(30)(31) Yellow autofluorescence of lignin in UV-light (40,41) has also been used as a criterion (28,30,31). However, in view of the countless autofluorescent substances in plants (40,42), even the recording of a typical emission spectrum for lignin (31) cannot be regarded as a specific proof for the presence of lignin.…”

Section: Evidence For the Participation Of Lignification In Resistancmentioning

confidence: 99%

Lignin Biosynthesis in Stem Rust Infected Wheat

Moerschbacher

1989

ACS Symposium Series

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Highly resistant wheat varieties exhibit a typical hypersensitive response when infected with an avirulent race of the stem rust fungus. Host cells which are penetrated by a fungal haustorium undergo rapid necrotization, thus depriving the biotrophic parasite of its nutritional basis. This rapid cell death is correlated with the deposition of lignin or lignin-like material in the host cell walls and protoplasts. Inhibition of lignin biosynthesis delays necrotization of penetrated host cells and partially breaks resistance of wheat to stem rust. In resistant plants, an increase in the activities of the general phenylpropanoid pathway and of the specific branch pathway of lignin biosynthesis can be detected at the time of the hypersensitive cell death, contrasting to decreased activities in susceptible near-isogenic plants. The participation of both an elicitor and a suppressor in the signal exchange between host and parasite has been suggested. An elicitor of lignification could be isolated from fungal cell walls. An endogenous suppressor of the resistance reaction was found in wheat cell walls.Lignin, the second most abundant organic compound on earth, is extremely resistant to microbial degradation (1,2) and thus constitutes one of the most effective mechanical barriers against pathogenic invasion (3,4). Consequently, the lignin content of higher plants has long been recognized as an important factor in the resistance against the attack by a myriad of potential pathogens.In addition to its role as a preformed resistance factor, Hijwegen (5) has also proposed active induction of lignification as a defense mechanism of cucumber against Cladosporium.Subsequently, in a number of hostpathogen interactions, induced lignification has been proposed as the active

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Ein Beitrag zur Kenntnis der hesperidinartigen Verbindungen und Flavone in den Pflanzen

Wasicky

Rotter

Thumer

1934

Archiv der Pharmazie

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Zur Kenntnis des SparteinsZ u s a m m e n f a s s u n g. 1. Es konnte gezeigt werden, dal3 bei den friiheren Verfahren zur Darstellung von Dehydrospartein stets eine Uberdehydrierung statt: findet, und dal3 Dehydrospartein von wechselnden Mengen Dis dehydrospartein beglcitet ist. 2. Unter besondercn Bedingungen ist die Dehydrobase in reinem Zustande darstellbar. Sie fuhrt bei der Hydrierung zu Spartein zuruck. 3. Das in der Literatur beschriebene Didehydrospartein ist kein einheitliches Produkt, sondern besteht aus einem Gemisch zweier Is05 meren, dem kristallisicrten a:Didehydrospartein und dem oligen BSDidehydrospartcin.4. Es gelang, eine einwandfreie Methode zur Trennung der beiden isomeren Didehydrosparteine zu finden. 5. Bei der Hydrierung der beiden Didehydrosparteine entstehen mindestcns zwei SparteinsIsomerc, von denen das askomere im Gegensatz zum Spartein eine gut kristallisierte Base darstellt. D i e s e r B e f u n d i s t u m s o b e m e r k e n s w e r t e r , a l s d i e s e s d i e e r s t e k r i s t a l l i s i e r t e S p a r t e i n b a s e i s t .6. das olige BSIsospartein weist in seinen Eigenschaften nahe Be: ziehungen zu dcm von C 1 e m o gefundenen dGSpartein auf.

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Fluoreszenzanalytische Untersuchungen an Pflanzen

Abstract: Es ist nStig, die Grenzen und Naehteile dieser qualitativen Methode einmal klar hervorzuheben. Vor allem ist festzustellen, dab man visuell hSehstens 30 bis 50~o der Gesamtfluoreszenz erfassen kann, wahrend der

Cited by 22 publications

References 13 publications

The estimation of free chloine in plants

The estimation of free chloine in plants

Lignin Biosynthesis in Stem Rust Infected Wheat

Ein Beitrag zur Kenntnis der hesperidinartigen Verbindungen und Flavone in den Pflanzen

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