Fluorescenzmikroskopischer Nachweis des menschlichen Y-Chromosoms in Interphasekernen durch Acridinderivate (?Atebrin?, ?Acranil?)

Evidentiation of the Male Chromatin Body in Human Peripheral Blood Leukocytes — A Quick Method for the Identification of Genetic Sex

“…The light source was a mercury vapor lamp HBO200; the excitor filter was the BG 12 and the barrier filter a 53/44 combination; the films have been obtained with Ilford HP4 and the prints with Agfa Gevaert Brovira 6, a high-contrast photographic paper. Table I reports the average percentage values obtained by Conen et al (1971) and Majewski et al (1971), referring to the presence of the F-body in lymphocytes and polymorphs from peripheral blood smears of male and female subjects, using Quinacrine Dihydrochloride.…”

“…Thanks to this elective affinity for some acridine derivatives, it has been possible to identify the Y-chromosome in interphase human nuclei. In fact, an intensely fluorescent body (F-body), corresponding to the distal part of the long arm of the Y-chromosome, is present in the nuclei of the oral mucosa of normal males and in cultured fibroblasts and lymphocytes (Pearson et al 1970, Moscetti and Mastroianni 1971, Majewski et al 1971). It has further been possible to evidence the F-body in human spermatozoa (Barlow and Vosa 1970) and in cells deriving from the amniotic fluid of male foetuses (Caspersson et al 1970).…”

Fluoreszenzmikroskopische Identifizierung von Anomalien des Y-Chromosoms

“…In manchen Zellen schien diese Region sogar etwas größer und sta-biler in der Fluoreszenz zu sein als bei dem regulären Y-Chromosom. Aus diesen Befunden schlossen wir, daß hier eine Translokation des stark fluoreszierenden langen Armes eines zweiten Y-Chromosoms auf den kurzen Arm eines Chromosoms der D-Gruppe vorlag (20) 30 Metaphasen nur eine mit einem Karyotyp 47,XYY, während 29 einen 45,X-Karyotyp aufwiesen. Bei diesem Patienten handelte es sich um einen 4 Wochen alten Säugling mit gemischter Gonadendysgenesie, Hypospadie, Testis rechts, Uterus, Streakgonade und Tube links.…”

“…Das Y-Körperchen wurde auch in Interphasekernen von anderen Geweben nachgewiesen, zum Beispiel in Lymphozyten, Fibroblasten, Haarwurzeizellen, Amnionzellen und Spermien (3,15,22,29,30,38,46,48).…”

Observations in human spermatozoa by fluorescent “Acranil” staining

“…On a previous work we proposed "Acranil" (A) fluorescent staining in the study of human chromosomes and later, with good results, in the recognition of Y heterochromatin in the nuclei of various human cell types in interphase (Moscetti and Mastroianni, 1971). Particularly good results were obtained with this fluorescent stain by Majewski et al (1971) in seeking the male chromatin body in the nuclei of cells from oral mucosa and amniotie fluid.…”