Fluorescently labelled glycans and their applications

Yan, Hongbin; Yalagala, Ravi Shekar; Yan, Fengyang

doi:10.1007/s10719-015-9611-9

Cited by 26 publications

(16 citation statements)

References 143 publications

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“…BODIPY fatty acids have been demonstrated as useful membrane probes ,. These fatty acids can be incorporated into cell membranes directly due to their amphiphilic nature, or undergo typical lipid transport and metabolism in cells .…”

Section: Figurementioning

confidence: 99%

“…BODIPY fatty acids have been demonstrated as useful membrane probes. [26,27] These fatty acids can be incorporated into cell membranes directly due to their amphiphilic nature, or undergo typical lipid transport and metabolism in cells. [28] In addition, BODIPY fluorophores are well known for their ability to form excimers, which causes a red shift in the fluorescence spectrum compared to the monomer form.…”

Section: Incorporation Of Bodipy Into Pc3 Cellsmentioning

confidence: 99%

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Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

et al. 2017

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The present study investigated the impact of 10 W microwave irradiation at 2.45 GHz on the cell viability and membrane properties of human prostate cancer PC3 cells, while the temperature of the cell cultures was maintained at 37±2 °C through simultaneous cooling. Microwave irradiation under these conditions did not induce apoptosis, nor otherwise lead to loss of cell viability, as indicated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), Annexin–V, and propidium iodide assays. Interestingly however, incorporation of BODIPY (4,4‐difluoro‐5‐methyl‐4‐bora‐3a,4a‐diaza‐s‐indacene) 3‐dodecanoic acid into the cell membrane was facilitated by microwave treatment at constant temperature. Also, constant temperature microwave treatment significantly increased the rate of reduction of MTT. Taken together, these results suggest that constant temperature microwave, while non‐lethal to PC3 cells, may alter membrane properties.

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Section: Figurementioning

confidence: 99%

Section: Incorporation Of Bodipy Into Pc3 Cellsmentioning

confidence: 99%

Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

et al. 2017

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“…A number of investigations were reported on porosity elimina tion in laser welding using various methods such as: application of a magnetic field on the weld pool [14][15][16], the use of a modulated beam [17], surface preparation [3,18], the use of hybrid laser-MlG or laser-TIG welding techniques [19], and the application of dual spots on the surface [20][21][22]. Only few investigations drew atten tion to porosity reduction by introducing a gap between the welded sheets.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Effect of Heat Input and Sheet Gap on Porosity Formation in Fillet Edge and Flange Couch Laser Welding of AC-170PX Aluminum Alloy for Automotive Component Manufacture

Alshaer

Mistry

2015

Journal of Manufacturing Science and Engineering

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U n d e rs ta n d in g th e E ffect of H e a t In p u t and S h e e t G ap on P o ro sity F o rm a tio n in F ille t Edge and F la n g e Couch L a s e r W e ld in g of A C -1 7 0 P X A lu m in u m A llo y fo r A u to m o tiv e C o m p o n en t M a n u fa c tu re An investigation is reported on the characteristics of porosity formation in high power disk laser welding o f AC-170PX (AA6014) alloy sheets (coated with titanium and zirconium) in two weld joint configurations: fillet edge and flange couch with AA4043 filler wire for potential automotive manufacturing applications. Porosity, macro-and microstructure characteristics, tensile strengths, microhardness, and joint geometry were investigated. It has been found that an increase in heat input and welding speed generates more porosity in both types of joints. The introduction of a 0.2 mm gap reduces porosity significantly in the fillet edge joints but it does not have noticeable effect on the flange couch joints. The mechanism of the porosity formation is discussed.

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“…14 Experimental data, such as titration measurement of binding constant of boronic acid ligand with hydrogen ion or cis-diol compound to give information concerning pK a or dissociation constant, have been the factual confirmations that there exists a jumping change around the pK a . 15,23,24,26,47,[50][51][52][53] And in the vicinity of the pK a value, different pH values could to the largest extent alter the binding force between the boronic acid ligand and the cis-diol compound. And the pH-adjusted binding strength could constitute the feature generation required in pattern recognition; that is, the different binding data from individual pHs could be used to encode the binding events as the selected features and then to discriminate the binding between cis-diol compounds with a boronic acid ligand by the pattern recognition algorithms.…”

Section: Resultsmentioning

confidence: 99%

Pattern Recognition of Monosaccharides via a Virtual Lectin Array Constructed by Boronate Affinity-Based pH-Featured Encoding

Liu

2015

Anal. Chem.

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Lectin array is an important tool in the fields of carbohydrate chemistry, glycobiology, and glycomics. Because natural lectins are associated with some apparent disadvantages such as tedious purification and easy loss of activity, artificial materials are applied to overcome such shortages by mimicking and replacing lectins in an artificial lectin array, among which boronate affinity-based materials are very outstanding and widely used. However, complicated synthetic works are often involved to design and create boronate affinity-based lectin-mimics. In this work, a facile and novel method was proposed to establish a virtual lectin array based on boronate affinity-based pH-featured encoding for discrimination of monosaccharides by pattern recognition. The dependence of boronate affinity on environmental pH was selected to encode each monosaccharide for feature generation, and the pH-featured encoding was used to construct the virtual lectin array. On the basis of the virtual array, pattern recognition algorithms were applied for data analysis. Monosaccharides were discriminated by principal component analysis, and the relations in the virtual lectin array were unraveled by cluster analysis. In this proof-of-concept work, without complicated synthesis or preparation, the proposed method was successful in mimicking lectin array and discriminating nine elementary monosaccharides found in nature, and it was also a new way of encoding in expanding the applications of boronate affinity-based materials and methods in the field of biomimetics.

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Fluorescently labelled glycans and their applications

Cited by 26 publications

References 143 publications

Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

Understanding the Effect of Heat Input and Sheet Gap on Porosity Formation in Fillet Edge and Flange Couch Laser Welding of AC-170PX Aluminum Alloy for Automotive Component Manufacture

Pattern Recognition of Monosaccharides via a Virtual Lectin Array Constructed by Boronate Affinity-Based pH-Featured Encoding

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