Fluorescent sensing of homocysteine by molecular imprinting

Chow, Cheuk Fai Stephen; Lam, Michael; Leung, Mitch K.P

doi:10.1016/s0003-2670(02)00520-2

Cited by 50 publications

(25 citation statements)

References 49 publications

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“…Amounts of imidocarb bound to the imprinted polymer were calculated by subtracting the amount of free substrate from the initial amount of the template. The adsorption capacity (Q), was calculated by the equation (1).…”

Section: Binding Of Imidocarb To Molecularly Imprinted Polymermentioning

confidence: 99%

“…Molecularly imprinted polymers are synthetic matrices that mimic biological receptor systems, which have the capability to bind target molecule with similar affinity and specificity to antibodies and enzymes [1,2]. This unique feature of MIPs was introduced during synthesis stage, where functional and crosslinking monomers are co-polymerized in the presence of a target analyte (the imprint molecule).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new potentiometric sensor based on molecularly imprinted polymer for analysis of a veterinary drug imidocarb dipropionate

Rizk

Toubar

Sayour³

et al. 2014

Eur. J. Chem.

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Section: Binding Of Imidocarb To Molecularly Imprinted Polymermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A new potentiometric sensor based on molecularly imprinted polymer for analysis of a veterinary drug imidocarb dipropionate

Rizk

Toubar

Sayour³

et al. 2014

Eur. J. Chem.

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“…Ye et al [19] performed MI on microgel spheres using a novel precipitation polymerization method. Chow et al [20] described a procedure for fluorescent sensing of homocysteine. The MIP were fabricated using the N-(1-pyrenyl)maleimide-DL-homocysteine adduct as template.…”

Section: Miniaturizationmentioning

confidence: 99%

Separation of biomolecules using electrophoresis and nanostructures

Kist¹,

Mandaji²

2004

Electrophoresis

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A large number of nanostructures have the potential to be used together with electrophoresis as separation media or separation additive in capillary electrophoresis, micellar electrokinetic chromatography, capillary electrochromatography, and other analytical techniques. Among those structures are nanotubes, nanocavities, nanowires, nanoposts, nanocones, nanospheres, molecular imprints, nanoparachutes (conical monodendrons), and general nanoparticles with random structures. This review is focused only on publications describing experimental works using molecular imprints, nanoposts, and nanospheres that are fabricated and applied for the purpose of separation media in electrophoresis-driven separations. The review follows an approximate chronological order in each section. As shown, the most popular are those resulting from molecular imprinting technologies. These biomimetic receptors are used in a great variety of fields, which includes electrophoresis, micellar electrokinetic chromatography, capillary electrochromatography, and other fields not reviewed in this work. A few examples of these other fields are, e.g., liquid chromatography, membranes, extractor or preconcentration techniques, immunosorbent assays, and sensing devices. The second topic scanned in the present work is the nanostructures that are used as obstacles to replace gels or polymers solutions in electrophoresis. Finally, the nascent field of nanospheres of gold and other materials as separation media is also reviewed.

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“…A series of analytical procedures based on high-performance liquid chromatography (HPLC) [6][7][8], gas chromatography-mass spectrometry (GC-MS) [9], enzymatic [10,11], immunoassay [12], molecular imprinting [13], and electrochemical [14] methods have been described to be effective for HCys determination during the past decade. However, tedious analytical procedures and expensive reagents and instrumentation are necessary for such determination methods.…”

Section: Introductionmentioning

confidence: 99%

Use of capillary electrophoresis with chemiluminescence detection for sensitive determination of homocysteine

Zhao

Huang

et al. 2011

J of Separation Science

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A sensitive capillary electrophoresis (CE) method with chemiluminescence (CL) detection was developed for the determination of homocysteine (HCys) in human plasma. In this work, N-(4-aminobutyl)-N-ethylisoluminol was used as tagging reagent to label the analyte for achieving high assay sensitivity. N-(4-Aminobutyl)-N-ethylisoluminol-tagged HCys after CE separation reacted with hydrogen peroxide in the presence of horseradish peroxidase, producing CL emission. Experimental conditions for labeling analyte, CE separation, and CL detection were studied. The CL intensity was proportional to the concentration of HCys in the range of 2.5×10(-8) to 5.0×10(-6) M. Detection limit (S/N=3) was 7.6×10(-9) M. Human plasma samples from healthy donors were analyzed by the presented method. HCys levels were found to be in the range of 9.50-15.3 μM.

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Fluorescent sensing of homocysteine by molecular imprinting

Cited by 50 publications

References 49 publications

A new potentiometric sensor based on molecularly imprinted polymer for analysis of a veterinary drug imidocarb dipropionate

A new potentiometric sensor based on molecularly imprinted polymer for analysis of a veterinary drug imidocarb dipropionate

Separation of biomolecules using electrophoresis and nanostructures

Use of capillary electrophoresis with chemiluminescence detection for sensitive determination of homocysteine

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