Separation of biomolecules using electrophoresis and nanostructures

Kist, Tarso B. Ledur; Mandaji, Marcos

doi:10.1002/elps.200406114

Cited by 39 publications

(24 citation statements)

References 43 publications

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“…Imprinted polymers are generated by polymerization of monomers and cross-linkers in the presence of a template [73]. The MIPs are expected to have high affinity for molecules resembling the template and can serve to extract such molecules (referred to as target molecules) from a complex mixture [74][75][76][77][78][79][80][81]. The interactions between the polymer and the target molecules are non-covalent interactions, and the affinity is largely based on the complementarity of the size, shape, electrostatics and hydrogen bonding of the MIP with the target molecule.…”

Section: Mammalian P450smentioning

confidence: 99%

Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates

Auclair

Polic

2015

Advances in Experimental Medicine and Biology

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Cytochrome P450 enzymes (P450s) have the ability to oxidize unactivated C-H bonds of substrates with remarkable regio-and stereoselectivity. Comparable selectivity for chemical oxidizing agents is typically difficult to achieve. Hence, there is an interest in exploiting P450s as potential biocatalysts. Despite their impressive attributes, the current use of P450s as biocatalysts is limited. While bacterial P450 enzymes typically show higher activity, they tend to be highly selective for one or a few substrates. On the other hand, mammalian P450s, especially the drugmetabolizing enzymes, display astonishing substrate promiscuity. However, product prediction continues to be challenging. This review discusses the use of small molecules for controlling P450 substrate specificity and product selectivity. The focus will be on two approaches in the area: (1) the use of decoy molecules, and (2) the application of substrate engineering to control oxidation by the enzyme.

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Section: Mammalian P450smentioning

confidence: 99%

Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates

Auclair

Polic

2015

Advances in Experimental Medicine and Biology

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“…Molecularly imprinted polymers (MIPs) are polymers assembled in the presence of a template. [60][61][62][63][64][65][66][67][68] After removal of the template, MIPs tend to show selective affinity for molecules containing the template moiety. This occurs mainly through noncovalent interactions such as complementarity in size, shape, electrostatics and hydrogen bonding.…”

Section: Covalent Modifications To Extend the Substrate Promiscuity Amentioning

confidence: 99%

Controlling substrate specificity and product regio- and stereo-selectivities of P450 enzymes without mutagenesis

Polic

Auclair

2014

Bioorganic & Medicinal Chemistry

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P450 enzymes (P450s) are well known for their ability to oxidize unactivated C-H bonds with high regio-and stereoselectivity. Hence, there is emerging interest in exploiting P450s as potential biocatalysts. Although bacterial P450s typically show higher activity than their mammalian counterparts, they tend to be more substrate selective. Most drug-metabolizing P450s on the other hand, display remarkable substrate promiscuity, yet product prediction remains challenging. Protein engineering is one established strategy to overcome these issues. A less explored, yet promising alternative involves substrate engineering. This review discusses the use of small molecules for controlling the substrate specificity and product selectivity of P450s. The focus is on two approaches, one taking advantage of non-covalent decoy molecules, and the other involving covalent substrate modifications

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“…A further progress in nanostructure based separation media or layer development has been reported in [4]. Nanopatterned surfaces (nanopillars) and nanoparticles have been already applied in electrophoretic and electrochromatographic separation [5]. A nanopatterned film has been prepared by molecular imprinting and used in electrophoretic separation.…”

Section: Introductionmentioning

confidence: 99%

Chemical Separation on Silver Nanorods Surface Monitored by TOF-SIMS

Petruš

Oriňák

Oriňáková

et al. 2017

Journal of Chemistry

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The article introduces a possible chemical separation of a mixture of two compounds on the metal nanorods surface. A silver nanorods surface has been prepared by controlled electrochemical deposition in anodic alumina oxide (AAO) template. Rhodamine 6G and 4-aminothiophenol have been directly applied to the sampling point on a silver nanorods surface in an aliquot mixture. The position of the resolved compounds was analysed by time-of-flight secondary ion mass spectrometry (TOF-SIMS) which measured the fragments and the molecular ions of the two compounds separated on the silver nanorods surface. Rhodamine 6G has been preconcentrated as 1.5 mm radial from the sampling point while 4-aminothiophenol formed a continuous self-assembled monolayer on the silver nanorods surface with a maximum molecular ion intensity at a distance of 0.5 mm from the sampling point. The separation of the single chemical components from the two-component mixture over the examined silver nanostructured films could clearly be shown. A fast separation on the mentioned nanotextured films was observed (within 50 s). This procedure can be easily integrated into the micro/nanofluidic systems or chips and different detection systems can be applied.

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Separation of biomolecules using electrophoresis and nanostructures

Cited by 39 publications

References 43 publications

Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates

Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates

Controlling substrate specificity and product regio- and stereo-selectivities of P450 enzymes without mutagenesis

Chemical Separation on Silver Nanorods Surface Monitored by TOF-SIMS

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