Fluorescent peptide probes for organophosphorus pesticides detection

Wang, Jianying; Zhang, Jiaying; Wang, Jing; Fang, Guozhen; Liu, Jifeng; Wang, Shuo

doi:10.1016/j.jhazmat.2020.122074

Cited by 103 publications

(30 citation statements)

References 40 publications

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“…Chemie enhanced absorbance from 190 nm to 200 nm and from 210 nm to 220 nm. These results indicated an increase in the ordered structure, including α-helix and β-sheet, and the decrease in unordered random coil structure, [22] which confirms the changes in the three-dimensional protein structure caused by photocleavage (Figure 3f).…”

Section: Methodssupporting

confidence: 61%

Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl‐Triggered Protein Photocleavage

et al. 2022

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The detection of environmental uranyl is attracting increasing attention. However, the available detection strategies mainly depend on the selective recognition of uranyl, which is subject to severe interference by coexisting metal ions. Herein, based on the unique uranyl-triggered photocleavage property, the protein BSA is labelled with fluorescent molecules that exhibit an aggregation-induced emission effect for uranyl detection. Uranyl-triggered photocleavage causes the separation of the fluorescent-molecule-labelled protein fragments, leading to attenuation of the emission fluorescence, which is used as a signal for uranyl detection. This detection strategy shows high selectivity for uranyl and an ultralow detection limit of 24 pM with a broad detection range covering five orders of magnitude. The detection method also shows high reliability and stability, making it a promising technique for practical applications in diverse environments.

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Section: Methodssupporting

confidence: 61%

Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl‐Triggered Protein Photocleavage

et al. 2022

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“…26 The sensing mechanism is based on the hydrolysis product of acetylthiocholine catalyzed by acetylcholinesterase (AChE), which was utilized to modulate the emission of AIEgens through the structural alternation. In addition, AIE fluorogens−SiO 2 −MnO 2 sandwich nanocomposites, 27 AIE nanoparticles−MnO 2 nanoflake, 28 tetraphenylethylene−peptide probes, 29 and AIE AuNCs 30 were developed recently for sensitive determination of OPs. The developed sensing systems are based on a AChE/acetylcholine/choline oxidase biosensor system, and the used enzymes are expensive and unstable for long-term conservation, which limits its further application for in situ detection.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

3D-Printed, Portable, Fluorescent-Sensing Platform for Smartphone-Capable Detection of Organophosphorus Residue Using Reaction-Based Aggregation Induced Emission Luminogens

et al. 2021

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Development of an easy-to-use, low-cost, household device can help the consumer quickly identify an organophosphorus (OP) residue concentration level. In this work, we demonstrate a 3D-printed, portable, fluorescent-sensing platform for smartphone-capable detection of OPs in vegetables. For development of the proposed device, we utilize the smartphone for capturing the strong thiol-activated fluorescence, which was produced by hydrolysis of OPs in the presence of alkali. The thiol-responsive AIEgen (maleimide-functionalized tetraphenylethylene) was non-emissive in both solution and the solid state but could be readily lighted up by the click addition of thiol to its MI pendant. An android application “Detection” has been developed on the basis of the gray value to analyze the different concentration levels of OPs in vegetable samples. The gray value was linearly related with the concentration of five kinds of organophosphorus residue, ranging from 0 to 20 μg/mL. It was also applied for determination of OPs residue in the leaves of cowpea, celery, and Chinese cabbage. Different from acetylcholinesterase enzyme-based sensors for poor stability under high temperature, the proposed method was a direct detection method for OPs and can be used for rapid monitoring of OPs residue concentration levels before LC-MS analysis.

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“…Of note, a strong pH dependence was also observed, consistent with supramolecular electrostatic interactions being the predominant underlying force that brings the sensor and pesticide into close proximity to enable effective energy transfer and sensitive pesticide detection. Reasonable detection limits for the analyte were determined (1.0 µmol/L), although other detection systems for methyl paraoxon have been reported with higher sensitivity (Wang et al, 2020e;Luo et al, 2020).…”

Section: Fluorescent Polymer-based Pesticide Detectionmentioning

confidence: 91%

Fluorescence-Based Sensing of Pesticides Using Supramolecular Chemistry

Levine

2021

Front. Chem.

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The detection of pesticides in real-world environments is a high priority for a broad range of applications, including in areas of public health, environmental remediation, and agricultural sustainability. While many methods for pesticide detection currently exist, the use of supramolecular fluorescence-based methods has significant practical advantages. Herein, we will review the use of fluorescence-based pesticide detection methods, with a particular focus on supramolecular chemistry-based methods. Illustrative examples that show how such methods have achieved success in real-world environments are also included, as are areas highlighted for future research and development.

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Fluorescent peptide probes for organophosphorus pesticides detection

Cited by 103 publications

References 40 publications

Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl‐Triggered Protein Photocleavage

Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl‐Triggered Protein Photocleavage

3D-Printed, Portable, Fluorescent-Sensing Platform for Smartphone-Capable Detection of Organophosphorus Residue Using Reaction-Based Aggregation Induced Emission Luminogens

Fluorescence-Based Sensing of Pesticides Using Supramolecular Chemistry

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