Fluorescent nanocrystals for use in early cervical cancer detection

Nida, Dawn; Rahman, Mohammed; Carlson, Kristen; Richards‐Kortum, Rebecca; Follen, Michelle

doi:10.1016/j.ygyno.2005.07.050

Cited by 62 publications

(43 citation statements)

References 29 publications

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“…However, traditional treatments for cancer cannot achieve the goal of tumor eradication because of their side effects and limitations. In recent years, with the rapid development of nanotechnology, it provides a bright prospect for cancer treatment [1,[20][21][22][23][24][25]. In particular, unique optical tunable gold nanomaterials show extensive and important potential in the field of biomedical application.…”

Section: Discussionmentioning

confidence: 99%

Photothermal Targetting therapy of A20 Mouse Lymphoma model using Anti CD-138 Antibody conjugated Gold Nanospheres

Yang¹,

Meng²,

Zong³

et al. 2014

J Hematol Thrombo Dis

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The aim of this study was to investigate the therapeutic effects of photothermal targeting of hollow gold nanospheres on lymphoma. Hollow gold nanospheres were modified by PEG (polyethylene glycol) and conjugated to anti-CD138 antibody. The conjugates were then injected into the A20 mouse lymphoma model followed by nearinfrared laser radiation (NIR). By examining the level of immunoglobulin G2a secreted by A20 cells and measuring the weight of specimen biopsy, tumorgenesis was determined. Finally, the possibility of lymphoma-targeted photothermal therapy was evaluated. Anti-CD138 antibody-conjugated hollow gold nanospheres (HAuNs-CD138) were prepared and the A20 mouse lymphoma model was established. After NIR treatment, A20 lymphoma growth was significantly suppressed. HAuNs-CD138 treatment represents a potential therapeutic strategy for CD138+ lymphoma.

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Section: Discussionmentioning

confidence: 99%

Photothermal Targetting therapy of A20 Mouse Lymphoma model using Anti CD-138 Antibody conjugated Gold Nanospheres

Yang¹,

Meng²,

Zong³

et al. 2014

J Hematol Thrombo Dis

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“…The smallest bars of group 7 are of width 2.2 μm, (b) Image of the resolution target obtained using a commercial confocal microscope (Lucid VivaScope 2500) with field of view 500 μm×500 μm. The region in (b) enclosed in the white rectangle represents the area imaged using our handheld reflectance confocal microscope antibodies specific to cancerous lesions, to serve as imaging contrast agents (Bugaj et al 2001;Hsu et al 2004;Nida et al 2005;Srinivas et al 2001). Such fluorescence detection schemes can potentially exhibit dramatically higher detection sensitivity and specificity, if the associated drug delivery problems can be adequately addressed.…”

Section: Discussion and Performance Analysismentioning

confidence: 99%

Handheld subcellular-resolution single-fiber confocal microscope using high-reflectivity two-axis vertical combdrive silicon microscanner

Kumar

Hoshino

Zhang

2008

Biomed Microdevices

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We introduce a handheld single-fiber laser-scanning confocal microscope, incorporating a high-reflectivity two-axis silicon vertical combdrive microscanner, aimed at in vivo early detection of epithelial precancers. The approach adopted is motivated by need for a portable, economical, biopsy-free, early precancer screening technology in low-infrastructure environments. Our microelectromechanical system (MEMS) based handheld probe integrates the microscanners with miniature objective lens system and flexible electrical routing in a forward-imaging configuration, with 4.8 mm distal probe tip outer diameter for unrestricted imaging access in biological sites such as the oral cavity and cervix. Reflectance confocal videos of a USAF 1951 resolution target and biological samples were obtained over 200 microm x 110 microm field of view, with 0.80 and 9.55 microm lateral and axial resolution, at 3.5-5.0 frames per second. With improvements to objective numerical aperture, our probe can enable precise evaluation of nuclear size, density, nucleus-to-cytoplasm ratio and cell density, which are important visual identifiers of epithelial precancers.

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“…Although the effectiveness of systemic administration of fluorescently labeled antibody has not been shown previously as a cancer-specific contrast agent in HNSCC, studies have explored the use of fluorescent imaging agents in the laboratory, and clinical setting optical imaging has been applied to detection of tumors using fluorescently labeled antibodies with success in Karposi's sarcoma and breast cancer (4,21). In addition to the attachment of fluorescent probes, anti-EGFR antibodies have been conjugated to quantum dots and gold nanoparticles to identify premalignant lesions of the cervix using topical application (22,23). Soukos et al (24) have used EGFR-targeted agents to detect premalignant lesions in the hamster cheek model after i.v.…”

Section: Discussionmentioning

confidence: 99%

Use of fluorescent labeled anti–epidermal growth factor receptor antibody to image head and neck squamous cell carcinoma xenografts

Rosenthal¹,

Kulbersh²,

King³

et al. 2007

Molecular Cancer Therapeutics

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Physicians and surgeons rely on subtle tissue changes to detect the extent of tumors and the presence of residual disease in the clinical setting. The development of a cancer-specific fluorescent contrast agent has the potential to provide real-time tumor imaging in the clinic or operating room. Because epidermal growth factor receptor (EGFR) is highly overexpressed on the surface of head and neck squamous cell carcinoma (HNSCC), we sought to determine if fluorescently labeled anti-EGFR antibody could be used to image HNSCC xenografts in vivo. Cetuximab or control isotype-matched IgG1 was conjugated with the Cy5.5 fluorochrome and systemically injected into mice bearing human split thickness skin grafts, tumor cell line xenografts, transplanted human tumor xenografts, or mouse mesothelioma tumors. Xenografts were imaged by time-domain fluorescence imaging or fluorescence stereomicroscopy. Both imaging modalities detected specific uptake of cetuximab-Cy5.5 in HNSCC xenografts with significantly higher fluorescence levels relative to control IgG1-Cy5.5. Tumor xenograft fluorescence was higher compared with background (before injection), human split thickness skin grafts, or mouse mesothelioma tumors at 24, 48, and 72 h. Fluorescence was detected in multiple HNSCC tumor cell lines with variable EGFR expression levels. Mock resections of flank tumors using fluorescence stereomicroscopy showed that small (2 mm) specimens could be detected in the surgical wound bed. These results show the feasibility of using fluorescently labeled anti-EGFR antibody to detect human tumors in the surgical setting. [Mol Cancer Ther 2007;6(4):1230 -8]

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Fluorescent nanocrystals for use in early cervical cancer detection

Cited by 62 publications

References 29 publications

Photothermal Targetting therapy of A20 Mouse Lymphoma model using Anti CD-138 Antibody conjugated Gold Nanospheres

Photothermal Targetting therapy of A20 Mouse Lymphoma model using Anti CD-138 Antibody conjugated Gold Nanospheres

Handheld subcellular-resolution single-fiber confocal microscope using high-reflectivity two-axis vertical combdrive silicon microscanner

Use of fluorescent labeled anti–epidermal growth factor receptor antibody to image head and neck squamous cell carcinoma xenografts

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