Fluorescent molecular nanocrystals anchored in sol–gel thin films: a label-free signalization function for biosensing applications

Dubuisson, Emilie; Szunerits, Sabine; Bacia, Maria; Pansu, Robert; Ibanez, Alain

doi:10.1039/c1nj20353c

Cited by 11 publications

(5 citation statements)

References 46 publications

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“…This amplification of the quenching efficiency has been particularly used for the detection of explosives [24][25][26] and in label free DNA chips. 27,28 This antenna effect can also be used to collect light energy and to concentrate it at the surface of the nanoparticles. 29…”

Section: Fluorescence Sensing Applicationsmentioning

confidence: 99%

Spectroscopy of BODIPY in solid phase: crystal and nanoparticles

Liao

Génot

Méallet‐Renault

et al. 2013

Phys. Chem. Chem. Phys.

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We compare the absorption and fluorescence spectra of single crystals and suspensions of nanoparticles with the prediction of the Frenkel theory. The single crystals of a novel synthesized fluorescent BODIPY derivative dye, adamantyl mesityl BODIPY (4,4-difluoro-3,5-di-(adamantyl)-8-mesityl-4-bora-3a,4a-diaza-s-indacene), have been prepared. Their birefringence and dichroism have been studied. The X-ray crystallography shows a monoclinic crystal with all transition moments parallel to one common plane. The refractive indices along the two neutral axes have been measured for a wavelength from 530 to 700 nm, with a difference, Δn equal to 0.11. The Frenkel exciton theory was used here to describe the coupling of the electronic excited states in the crystals. The coupling estimated by the dipolar approximation was compared with the excited state splitting calculated by TDDFT in dimers. A perfect crystal absorption spectrum is predicted. The spectral broadening that occurs at room temperature is also taken into account. The absorption spectrum of the monocrystal is reproduced without adjustable parameters. But we had to take into account the presence of optical leaks in our microspectrophotometer before comparing experiment and theory. The controlled size nanoparticles (NPs) produced by our 3D hydrodynamic focusing microfluidic system exhibit molecule like absorption. We could reproduce their absorption and fluorescence spectra assuming a strong disorder in the Frenkel model. We conclude that the nanoparticles are amorphous.

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Section: Fluorescence Sensing Applicationsmentioning

confidence: 99%

Spectroscopy of BODIPY in solid phase: crystal and nanoparticles

Liao

Génot

Méallet‐Renault

et al. 2013

Phys. Chem. Chem. Phys.

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“…Moreover, FONs can be modified with biorecognition molecules to enable the detection of various targets. For instance, Dubuisson et al reported a rhodamine B (Rb)-based fluorescent biosensor for DNA detection [ 178 ]. In their work, Rb FONs were modified with a quencher molecule (Cy5)-labeled DNA, resulting in fluorescence quenching through the Förster resonance energy transfer (FRET) process.…”

Section: Fluorescent Assaysmentioning

confidence: 99%

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Deng,

Chang,

Liu

et al. 2023

Biosensors

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Over the past few decades, molecular self-assembly has witnessed tremendous progress in a variety of biosensing and biomedical applications. In particular, self-assembled nanostructures of small organic molecules and peptides with intriguing characteristics (e.g., structure tailoring, facile processability, and excellent biocompatibility) have shown outstanding potential in the development of various biosensors. In this review, we introduced the unique properties of self-assembled nanostructures with small organic molecules and peptides for biosensing applications. We first discussed the applications of such nanostructures in electrochemical biosensors as electrode supports for enzymes and cells and as signal labels with a large number of electroactive units for signal amplification. Secondly, the utilization of fluorescent nanomaterials by self-assembled dyes or peptides was introduced. Thereinto, typical examples based on target-responsive aggregation-induced emission and decomposition-induced fluorescent enhancement were discussed. Finally, the applications of self-assembled nanomaterials in the colorimetric assays were summarized. We also briefly addressed the challenges and future prospects of biosensors based on self-assembled nanostructures.

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“…1) functionalized with fluorescein labeled peptides for developing novel bioprobes 207 and rhodamine based nanocrystals anchored in a sol-gel film and grafted with hairpin shaped DNA for detection of the complementary DNA. 208 Aggregation induced enhancement of fluorescence has been exploited in the development of organic nanoparticles useful in bioimaging applications. 10H-phenothiazine based donor-acceptor molecules upon protonation and aggregation form fluorescent nanoparticles in the lysosomes of cancer cells but not in normal cells, facilitating selective imaging.…”

Section: Medicinementioning

confidence: 99%

Optical materials based on molecular nanoparticles

2012

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A major part of contemporary nanomaterials research is focused on metal and semiconductor nanoparticles, constituted of extended lattices of atoms or ions. Molecular nanoparticles assembled from small molecules through non-covalent interactions are relatively less explored but equally fascinating materials. Their unique and versatile characteristics have attracted considerable attention in recent years, establishing their identity and status as a novel class of nanomaterials. Optical characteristics of molecular nanoparticles capture the essence of their nanoscale features and form the basis of a variety of applications. This review describes the advances made in the field of fabrication of molecular nanoparticles, the wide spectrum of their optical and nonlinear optical characteristics and explorations of the potential applications that exploit their unique optical attributes.

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Fluorescent molecular nanocrystals anchored in sol–gel thin films: a label-free signalization function for biosensing applications

Cited by 11 publications

References 46 publications

Spectroscopy of BODIPY in solid phase: crystal and nanoparticles

Spectroscopy of BODIPY in solid phase: crystal and nanoparticles

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Optical materials based on molecular nanoparticles

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