Fluorescent Molecular Imaging and Dosimetry Tools in Photodynamic Therapy

Pogue, Brian W.; Samkoe, Kimberley S.; Gibbs-Strauss, Summer L.; Davis, Scott C.

doi:10.1007/978-1-60761-697-9_15

Cited by 8 publications

(9 citation statements)

References 49 publications

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“…Therefore, one important method of encouraging high selectivity of aPDI for microbial cells over surrounding host mammalian cells is to employ local delivery and a short-drug light interval. Since the majority of PSs are very fluorescent, it is common to carry out quantitative fluorescent imaging to confirm that there is a sufficient accumulation of PS in the target lesion [48]. …”

Section: Light Sources Optics and Dosimetrymentioning

confidence: 99%

Advances in antimicrobial photodynamic inactivation at the nanoscale

2017

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The alarming worldwide increase in antibiotic resistance amongst microbial pathogens necessitates a search for new antimicrobial techniques, which will not be affected by, or indeed cause resistance themselves. Light-mediated photoinactivation is one such technique that takes advantage of the whole spectrum of light to destroy a broad spectrum of pathogens. Many of these photoinactivation techniques rely on the participation of a diverse range of nanoparticles and nanostructures that have dimensions very similar to the wavelength of light. Photodynamic inactivation relies on the photochemical production of singlet oxygen from photosensitizing dyes (type II pathway) that can benefit remarkably from formulation in nanoparticle-based drug delivery vehicles. Fullerenes are a closed-cage carbon allotrope nanoparticle with a high absorption coefficient and triplet yield. Their photochemistry is highly dependent on microenvironment, and can be type II in organic solvents and type I (hydroxyl radicals) in a biological milieu. Titanium dioxide nanoparticles act as a large band-gap semiconductor that can carry out photo-induced electron transfer under ultraviolet A light and can also produce reactive oxygen species that kill microbial cells. We discuss some recent studies in which quite remarkable potentiation of microbial killing (up to six logs) can be obtained by the addition of simple inorganic salts such as the non-toxic sodium/potassium iodide, bromide, nitrite, and even the toxic sodium azide. Interesting mechanistic insights were obtained to explain this increased killing.

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Section: Light Sources Optics and Dosimetrymentioning

confidence: 99%

Advances in antimicrobial photodynamic inactivation at the nanoscale

2017

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“…31 The widely used ultrasound imaging and photoacoustic are highly synergistic as they can provide anatomical and functional information simultaneously utilizing the same receiver (transducer) and associated electronics. [23][24][25][26][27][28][29][30][31] Methylene blue (optical absorption peak around 670 nm) with singlet oxygen quantum yield of 0.52 is used as a photosensitizer in this study. 35 Methylene blue has low fluorescence quantum yield (0.04), however it is a good photoacoustic contrast agent with optical absorption coefficient ~95000 M -1 cm -1 .…”

Section: Introductionmentioning

confidence: 99%

“…Therefore the concern associated with fluorescence guided PDT dosimetry is that depth-resolved information on distribution of the photosensitizer cannot be obtained either with fiber optic probe point measurements or whole body fluorescence imaging as penetration depth is limited due to scattering of both excitation light and fluorescence. 23 Photoacoustic imaging (PAI) could address the need for a noninvasive imaging technique that can provide depth dependent 3D information on PS heterogeneous distribution in tumor at ultrasonic resolutions.…”

Section: Introductionmentioning

confidence: 99%

Towards image-guided photodynamic therapy of Glioblastoma

Mallidi¹,

Huang²,

Liu

et al. 2013

SPIE Proceedings

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Glioblastoma (GBM) is an aggressive cancer with dismal survival rates and few new treatment options. Fluorescence guided resection of GBM followed by photodynamic therapy (PDT) has shown promise in several chemo-or radiotherapy non-responsive GBM treatments clinically. PDT is an emerging light and photosensitizer (PS) mediated cytotoxic method. However, as with other therapeutic modalities, the outcomes are variable largely due to the nonpersonalization of dose parameters. The variability can be attributed to the differences in heterogeneous photosensitizer accumulation in tumors. Building upon our previous findings on utilizing PS fluorescence for designing tumor-specific PDT dose, we explore the use of photoacoustic imaging, a technique that provides contrast based on the tissue optical absorption properties, to obtain 3D information on the tumoral photosensitizer accumulation. The findings of this study will form the basis for customized photodynamic therapy for glioblastoma and have the potential to serve as a platform for treatment of other cancers.

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“…It is known that resonant CARS signal is proportional to the square of induced third-order polarization, namely P (3) as shown in Eq. (1).…”

Section: Design Ideas and Design Requirmentsmentioning

confidence: 99%

“…Obtaining molecular imaging in living organisms is applicable to the diagnosis of diseases such as cancer and cardiovascular disease [1][2][3]. This imaging technique can also be utilized in treating these kinds of disorders by testing the clinical effects of new medication.…”

Section: Introductionmentioning

confidence: 99%

The Optical Design of Probe-type Microscope Objective for Intravital Laser Scanning CARS Microendoscopy

Rim¹

2010

Journal of the Optical Society of Korea

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A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ωp and ωS. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

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Fluorescent Molecular Imaging and Dosimetry Tools in Photodynamic Therapy

Cited by 8 publications

References 49 publications

Advances in antimicrobial photodynamic inactivation at the nanoscale

Advances in antimicrobial photodynamic inactivation at the nanoscale

Towards image-guided photodynamic therapy of Glioblastoma

The Optical Design of Probe-type Microscope Objective for Intravital Laser Scanning CARS Microendoscopy

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