Fluorescent Magnetic Nanoprobes for in vivo Targeted Imaging and Hyperthermia Therapy of Prostate Cancer

As a new generation of fluorophores, up-conversion nanoparticles (UCNPs) exhibit high penetration depth in tissues, low photodamage to biological samples, large Stokes shifts, sharp emission bands, free auto-fluorescence background and high photostability, thus making them an ideal kind of fluorescent labels for a variety of assay formats ranging from bio-imaging to cancer therapy. In this article, the method for the synthesis of biocompatible UCNPs, the recent progress regarding the UCNPs for multimodal targeted tumor imaging, drug delivery, photodynamic therapy (PDT), and photothermal therapy (PTT) are discussed on emphasis. The challenges and opportunities in this emerging field are also presented.

Section: Ucnps Used For Gastric Cancer Imagingmentioning

confidence: 94%

Up-Conversion Nanoparticles for Gastric Cancer Targeted Imaging and Therapy

Yang¹,

Han²,

Yue³

2016

“…Experiments demonstrated that this method is advantageous for cell-based assays because of automated manipulation of multiple reagents in addition to reduced reagent and analysis time. In 2010, Jiajia Ji et al reviews advances of nanotechnology in the stem cells research, including various micro/nanofabrication technologies, microgrooves technology and so on [24].…”

Section: Cell Analysismentioning

confidence: 99%

“…There are several new techniques to analyze the cell, such as new capillary electrophoresis, molecular imaging [20,21], single walled carbon nanotubes [22] and microfluidic chip. Conventional flow cytometers which are routinely utilized for analyzing the physical and chemical properties of biological cells require a high amount of reagent for analysis and furthermore are both bulky and expensive and require trained personnel for operation and maintenance.…”

Section: Cell Analysismentioning

confidence: 99%

Biological Application of Digital Microfluidics Technology

Liu¹,

Deng²,

Qin³

et al. 2010

Digital microfluidics technology offers a platform for developing diagnostic applications with the advantages of portability, sample and reagent volume reduction, faster analysis, increased automation, low power consumption, compatibility with mass manufacturing and high throughput. In addition to diagnostics, digital microfluidics is finding use in nucleic acid analysis, peptide and protein analysis, cell analysis, drug analysis and delivery and immunization analysis. In this review, we describe these applications, their implementation, and associated design issues. As other review in the digital microfluidics technology, there have been and will be unexpected developments as DMF matures, but we predict that the future is bright for this promising technology at the last section.Keywords: Digital microfluidics technology; Nucleic acid analysis; Peptide and protein analysis; Cell analysis; Drug analysis and delivery Citation: B. Liu et al. Biological Application of Digital Microfluidics Technology. Nano Biomed Eng. 2010, 2(2), 149-154. DOI: 10.5101/nbe.v2i2.p149-154. The advent and development of digital microfluidics technologyTraditional (continuous-flow) microfluidic technologies are based on the continuous flow of liquid through microfabricated channels [1]. Continuous-flow systems are inherently difficult to integrate because the parameters that govern flow field (e.g. pressure, fluid resistance, electric field strength) vary along the flow-path, making the flow at any location dependent upon the properties of the entire system.The concept of digital microfluidics (DMF) arose in the late 1990s and involves the manipulation of discrete volumes of liquids on a surface. Manipulation of droplets can occur through various mechanisms, including electrowetting [2], dielectrophoresis [3], thermocapillary transport [4] and surface acoustic wave transport [5]. DMF was popularized in the early 2000s by Fair and coworkers [6] and Kim and coworkers [7] at Duke and UCLA, respectively. The technique was explained as being a phenomenon driven by surface tension, and was called "electrowetting" or "electrowetting ondielectric" (EWOD). A detailed review of electrowetting basics can be found in the work of Mugele [8]. In addition, work on simulation and modeling of dropletbased electrowetting has been reported by Biddut Bhattacharjee and Homayoun Najjaran [9].In contrast to continuous-flow biochips, digital microfluidic biochips platform is under software-driven electronic control, eliminating the need for mechanical tubes, pumps, and valves. Moreover, because each droplet can be controlled independently, these "digital" systems also have dynamic reconfigurability, whereby groups of cells in a microfluidic array can be reconfigured to change their functionality during the concur- Application of digital microfluidics technology in bioanalysisTo accomplish a digital microfluidics device it requires a hierarchical taxonomy. At the top level, applications are scaled to a microfluidic platform. The second level describ...

“…Currently, nanoparticles-based techniques shows great promise in applications of bioassay and biomedical [1][2][3][4][5][6]. Dye-doped silica nanoparticle have many unique properties such as highly fluorescent intensity, highly photostability, and excellent biological compatibility [7,8].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Ru(bpy)3 -doped Silica Nanoparticle and Its Application in Fluorescent Immunoassay

Yin¹,

Liu²,

Zhang³

et al. 2010

A novel type of amino functionalized core-shell Ru(bpy) 3 -doped silica nanoparticles was synthesized using a simple and effective approach of reverse microemulsion. The nanoparticles were characterized by transmission electron microscope, fluorescence spectra, UV-Vis spectroscopy and tests of photostability and dye molecular leakage. It was found that the nanoparticles exhibit excellent fluorescent properties such as extremely bright, highly photostable and chemicalstable. Furthermore, the nanoparticles utilized as a fluorescent marker applying in fluorescent immunoassay of mouse IgG were studied and desired results were obtained.