Fluorescent labeling and tracking of nanoclay

Díaz, Carlos A.; Xia, Yining; Rubino, Maria; Auras, Rafael; Jayaraman, K.; Hotchkiss, Joseph H.

doi:10.1039/c2nr32978f

Cited by 38 publications

(27 citation statements)

References 16 publications

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“…For imaging of kaolin on collagen, fluorescent labeling of kaolin particles was carried out in a two-step reaction (23). Kaolin was mixed with 3-mercaptopropyl-trimethoxysilane in 80% methanol (50 mL methanol/g kaolin) in a 3:1 mass ratio.…”

Section: Methodsmentioning

confidence: 99%

Contact activation of blood coagulation on a defined kaolin/collagen surface in a microfluidic assay

Zhu

Diamond

2014

Thrombosis Research

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Generation of active Factor XII (FXIIa) triggers blood clotting on artificial surfaces and may also enhance intravascular thrombosis. We developed a patterned kaolin (0 to 0.3 pg/μm2)/type 1 collagen fibril surface for controlled microfluidic clotting assays. Perfusion of whole blood (treated only with a low level of 4 μg/mL of the XIIa inhibitor, corn trypsin inhibitor) drove platelet deposition followed by fibrin formation. At venous wall shear rate (100 s−1), kaolin accelerated onset of fibrin formation by ~100 sec when compared to collagen alone (250 sec vs. 350 sec), with little effect on platelet deposition. Even with kaolin present, arterial wall shear rate (1000 s−1) delayed and suppressed fibrin formation compared to venous wall shear rate. A comparison of surfaces for extrinsic activation (tissue factor TF/collagen) versus contact activation (kaolin/collagen) that each generated equal platelet deposition at 100 s−1 revealed: (1) TF surfaces promoted much faster fibrin onset (at 100 sec) and more endpoint fibrin at 600 sec at either 100 s−1 or 1000 s−1, and (2) kaolin and TF surfaces had a similar sensitivity for reduced fibrin deposition at 1000 s−1 (compared to fibrin formed at 100 s−1) despite differing coagulation triggers. Anti-platelet drugs inhibiting P2Y1, P2Y12, cyclooxygenase-1 or activating IP-receptor or guanylate cyclase reduced platelet and fibrin deposition on kaolin/collagen. Since FXIIa or FXIa inhibition may offer safe antithrombotic therapy, especially for biomaterial thrombosis, these defined collagen/kaolin surfaces may prove useful in drug screening tests or in clinical diagnostic assays of blood under flow conditions.

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Section: Methodsmentioning

confidence: 99%

Contact activation of blood coagulation on a defined kaolin/collagen surface in a microfluidic assay

Zhu

Diamond

2014

Thrombosis Research

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“…Rhodamine B released in incubation samples is unlikely to affect natural chromatic features of any microorganism because this fluorescent dye has been shown to stain only the mitochondria (e.g., Johnson, Walsh, & Chen, 1980;Reungpatthanaphong et al, 2003;Scaduto & Grotyohann, 1999), which are not detectable in a PIV system at current camera resolutions. If microorganisms with emission wavelengths higher than 550 nm are used, other fluorescent dyes with emission wavelengths lower than 550 nm and compatible with minerals (e.g., fluorescein; Diaz et al, 2013) can be used.…”

Section: Discussionmentioning

confidence: 99%

Optical Measurement of Cell Colonization Patterns on Individual Suspended Sediment Aggregates

2017

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Microbial processes can make substantial differences to the way in which particles settle in aquatic environments. A novel method (OMCEC, optical measurement of cell colonization) is introduced to systematically map the biological spatial distribution over individual suspended sediment aggregates settling through a water column. OMCEC was used to investigate (1) whether a carbon source concentration has an impact on cell colonization, (2) how cells colonize minerals, and (3) if a correlation between colonization patterns and aggregate geometry exists. Incubations of Saccharomyces cerevisiae and stained montmorillonite at four sucrose concentrations were tested in a settling column equipped with a full‐color microparticle image velocimetry system. The acquired high‐resolution images were processed to map the cell distribution on aggregates based on emission spectra separation. The likelihood of cells colonizing minerals increased with increasing sucrose concentration. Colonization patterns were classified into (i) scattered, (ii) well touched, and (iii) poorly touched, with the second being predominant. Cell clusters in well‐touched patterns were found to have lower capacity dimension than those in other patterns, while the capacity dimension of the corresponding aggregates was relatively high. A strong correlation of colonization patterns with aggregate biomass fraction and properties suggests dynamic colonization mechanisms from cell attachment to minerals, to joining of isolated cell clusters, and finally cell growth over the entire aggregate. This paper introduces a widely applicable method for analyses of microbial‐affected sediment dynamics and highlights the microbial control on aggregate geometry, which can improve the prediction of large‐scale morphodynamics processes.

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“…Farhoodi et al [101] показали, что алюминий и кремний могут мигрировать из PETбутылок в кислой среде при 25 и 45 С. В других исследованиях выявлено, что как алюминий, так и кремний могут мигрировать из нанокомпозитов в водные и водно-этанольные среды [103].…”

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