Fluorescent Gold Nanoprobe Sensitive to Intracellular Reactive Oxygen Species

Abstract

Gold nanoprobes immobilized with fluorescein‐hyaluronic acid (HA) conjugates are fabricated and utilized for monitoring intracellular reactive oxygen species (ROS) generation in live cells via nanoparticle surface energy transfer. A bio‐inspired adhesive molecule, dopamine, is used to robustly end‐immobilize HA onto the surface of gold nanoparticles (AuNPs) for securing intracellular stability against glutathione. ROS induces cleavage and fragmentation of the HA chains immobilized on the surface of the AuNPs allows rapid and specific detection of intracellular ROS by emitting strong fluorescence‐recovery signals. In particular, fluorescence‐quenched gold nanoprobes exhibit selective and dose‐dependent fluorescence‐recovery signals upon exposure to certain oxygen species such as superoxide anion (${\rm O}_{2}^{\cdot{{-\!\!\!\!\!-}}}$) and hydroxyl radical (·OH). The fluorescent gold nanoprobe is usefully exploited for real‐time intracellular ROS detection and antioxidant screening assay, and has exciting potential for various biomedical applications as a new class of ROS imaging probes.

A Microfluidic DNA Sensor Based on Three-Dimensional (3D) Hierarchical MoS2/Carbon Nanotube Nanocomposites

“…Over the last two decades, various nanomaterials including graphene oxide [1,2,3,4], Au nanoparticles [5,6,7], metal-organic framework (MOF) [8,9,10], carbon nanotubes [11,12], and MoS 2 nanosheets [13,14,15,16,17,18,19] have been synthesized to develop nanomaterial-based biosensors for a variety of applications. In particular, there has been increasing interest in developing rapid, cost-effective, and sensitive methods for DNA detection because of its vital importance in molecular diagnostics, environmental monitoring, and anti-bioterrorism [3].…”

Highly Selective and Ultrasensitive Turn-on Luminescence Chemosensor for Mercury (II) Determination Based on the Rhodamine 6G Derivative FC1 and Au Nanoparticles

“…Interestingly, they are excellent quenchers of organic fluorophores via resonance energy transfer (RET) and can efficiently quench the molecular-excitation energy in the chromophore–AuNP composite [27]. In this sense, the quenching property of AuNPs has been employed in several chemosensing schemes [28,29], and many approaches have been used for Hg 2+ determination using AuNPs, being an area of current interest [30,31,32,33,34,35,36].…”

Near‐Infrared‐ and pH‐Responsive System for Reversible Cell Adhesion using Graphene/Gold Nanorods Functionalized with i‐Motif DNA

“…The AuNRs, with a longitudinal absorption band at 810 nm, were prepared (Figure S2) and immobilized on the surface using a Au-catechol interaction. [26] It has been reported that the interaction force between catechol and Au is comparable to that of streptavidin and biotin. [27] The prepared PDA-RGO modified ITO surfaces (PDA-RGO/ITO) were characterized by X-ray photoelectron spectroscopy (XPS; Figure S3 a), which showed the characteristic peaks of C1s, N1s, and O1s.…”