Fluorescent determination of trinitrotoluene with bovine serum albumin mediated enhancement of thioglycolic acid capped cadmium selenium quantum dots

Devi, Sarita; Tyagi, Sachin

doi:10.1080/10739149.2018.1531019

Cited by 9 publications

(9 citation statements)

References 46 publications

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“…As expected, the fluorescence intensity of VEGF-N,S-CDs increased at a higher VEGF concentration used for its conjugation to the N,S-CDs. , The enhancement in the fluorescence intensity of VEGF-N,S-CDs at a higher VEGF conjugation density occurred due to the stronger surface passivation of CDs and higher surface hydrophobicity as a result of elimination of some nonradiative transitions ,, or suppressed quenching resulting from interactions with solvent or ions. A similar effect was observed for semiconductor and carbon quantum dots conjugated to proteins …”

Section: Results and Discussionsupporting

confidence: 77%

“…A similar effect was observed for semiconductor and carbon quantum dots conjugated to proteins. 61 Both VEGF-N,S-CDs and N,S-CDs were imaged using transmission electron microscopy (TEM), which revealed that N,S-CDs were 2−4 nm in diameter (Figure 4A), while the size of VEGF-N,S-CDs was in the range of 5−7 nm (Figure 4B). The conjugation of N,S-CDs to VEGF did not affect their spherical shape and dispersity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

et al. 2020

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One of the challenges of using growth factors for tissue regeneration is to monitor their biodistributions and delivery to injured tissues for minimally invasive detection. In the present study, tracking of human vascular endothelial growth factor (VEGF) was achieved by chemically linking it to photoluminescent carbon dots (CDs). Carbon dots were synthesized by the hydrothermal method and, subsequently, conjugated with VEGF using carbodiimide coupling. ELISA and western blot analysis revealed that VEGF-conjugated CDs preserve the binding affinity of VEGF to its antibodies. We also show that VEGF-conjugated CDs maintain the functionality of VEGF for tube formation and cell migration. The VEGF-conjugated CDs were also used for in vitro imaging of human umbilical vein endothelial cells. The results of this work suggest that cell-penetrating VEGF-conjugated CDs can be used for growth factor protein tracking in therapeutic and tissue engineering applications.

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Section: Results and Discussionsupporting

confidence: 77%

Section: ■ Results and Discussionmentioning

confidence: 99%

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

et al. 2020

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“…Basiri et al found the conjugation of VEGF resulted in varying degrees of fluorescence quenching depending on the concentration of the aptamer (Basiri et al, 2020). A similar effect has been seen in conjugation of proteins to semiconductor and carbon quantum dots (Devi and Tyagi, 2019) and demonstrates the importance of considering such factors during the functionalization of CDs.…”

Section: Key Parameters For Anti-vegf CD Synthesismentioning

confidence: 77%

Carbon Dots Fabrication: Ocular Imaging and Therapeutic Potential

Garner

Vichare

Paulson

et al. 2020

Front. Bioeng. Biotechnol.

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Vision loss is a major complication in common ocular infections and diseases such as bacterial keratitis, age-related macular degeneration (AMD) and diabetic retinopathy (DR). The prevalence of such ophthalmic diseases represents an urgent need to develop safe, effective, and long-term treatments. Current therapies are riddled with drawbacks and limitations which calls for the exploration of alternative drug delivery mechanisms. Toxicity of the inorganic metals and metal oxides used for drug delivery raise safety concerns that are alleviated with the alternate use of, a natural and organic polymer which is both biocompatible and environmentally friendly. Carbon dots (CDs) represent a great potential in novel biomedical applications due to their tunable fluorescence, biocompatibility, and ability to be conjugated with diverse therapeutic materials. There is a growing interest on the exploitation of these properties for drug delivery with enhanced bio-imaging. However, there are limited reports of CD applications for ophthalmic indications. In this review, we focus on the CD potential and the development of translational therapies for ophthalmic diseases. The current review presents better understanding of fabrication of CDs and how it may be useful in delivering anti-bacterial agents, anti-VEGF molecules as well as imaging for ophthalmic applications.

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“…The trace detection of explosive compounds, mainly the trinitrotoluene (TNT), with high sensitivity and selectivity is highly obliged for public security and environmental safety [1]. Among the presently available various sensing strategies, the fluorescence quenching-based methods emerged as a portable and handheld method offering the high selectivity and sensitivity [2], although different nanomaterials such as metallic nanoparticles [3][4][5], quantum dots (QDs) [6,7], and conjugated polymers [8][9][10][11] have been explored for the nitroexplosive sensing in the aqueous phase. But these probes possess various limitations such as poor stability at ambient conditions, polydispersity, photobleaching, and high interference with the TNT analogs.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the nitrogen-doped carbon QDs (NCQDs) also has been reported for the TNT detection based on the same mechanism [6,7,18]. Therefore, a new platform can be designed by conjugating the IRMOF-3 particles with NCQDs to achieve the more robust, efficient, and selective sensing material for TNT over TNP.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-doped carbon quantum dots conjugated isoreticular metal-organic framework-3 particles based luminescent probe for selective sensing of trinitrotoluene explosive

et al. 2020

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Amine group-containing isoreticular metal-organic framework (IRMOF-3) particles are utilized for the first time as a trinitrotoluene (TNT) sensing material. IRMOF-3 particles are synthesized using zinc nitrate as a metal precursor and 2-amino-1,4benzenedicarboxylic acid as a linker. The nitrogen-doped carbon quantum dots (NCQDs) are synthesized from citric acid and ethylenediamine as carbon and nitrogen precursor, respectively. The NCQDs are conjugated with IRMOF-3 particles as IRMOF-3/NCQDs. The TEM micrograph revealed the average size of IRMOF-3 particles to be 363.66 nm. The photoluminescence emission intensity of IRMOF-3 particles at λ em 430 nm is highly increased in the presence of NCQDs (λ ex 330 nm). Both the assynthesized IRMOF-3 and IRMOF-3/NCQD particles are explored for TNT detection to compare the effect of NCQDs on the IRMOF-3 particle surface. Lower limit of detection (7.5 × 10 −8 M) and higher Stern-Volmer constant (4.46 × 10 6 M −1 ) are achieved by IRMOF-3/NCQD particles. The association constant also increased from 5.3 × 10 4 to 2.78 × 10 6 M −1 after the conjugation of IRMOF-3 particles with NCQDs. Moreover, enhanced selectivity for TNT over trinitrophenol is achieved using the IRMOF-3/NCQD particles.

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Fluorescent determination of trinitrotoluene with bovine serum albumin mediated enhancement of thioglycolic acid capped cadmium selenium quantum dots

Cited by 9 publications

References 46 publications

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

Carbon Dots Fabrication: Ocular Imaging and Therapeutic Potential

Nitrogen-doped carbon quantum dots conjugated isoreticular metal-organic framework-3 particles based luminescent probe for selective sensing of trinitrotoluene explosive

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