Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

Basiri, Hamideh; Mehrizi, Ali Abouei; Ghaee, Azadeh; Farokhi, Mehdi; Chekini, Mahshid; Kumacheva, Eugenia

doi:10.1021/acs.langmuir.9b03980

Cited by 27 publications

(21 citation statements)

References 63 publications

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“…Ideally, surface functionalization should be achieved in a way that preserves the photoluminescence properties of the CDs for bioimaging. Basiri et al found the conjugation of VEGF resulted in varying degrees of fluorescence quenching depending on the concentration of the aptamer (Basiri et al, 2020). A similar effect has been seen in conjugation of proteins to semiconductor and carbon quantum dots (Devi and Tyagi, 2019) and demonstrates the importance of considering such factors during the functionalization of CDs.…”

Section: Key Parameters For Anti-vegf CD Synthesismentioning

confidence: 78%

Carbon Dots Fabrication: Ocular Imaging and Therapeutic Potential

Garner

Vichare

Paulson

et al. 2020

Front. Bioeng. Biotechnol.

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Vision loss is a major complication in common ocular infections and diseases such as bacterial keratitis, age-related macular degeneration (AMD) and diabetic retinopathy (DR). The prevalence of such ophthalmic diseases represents an urgent need to develop safe, effective, and long-term treatments. Current therapies are riddled with drawbacks and limitations which calls for the exploration of alternative drug delivery mechanisms. Toxicity of the inorganic metals and metal oxides used for drug delivery raise safety concerns that are alleviated with the alternate use of, a natural and organic polymer which is both biocompatible and environmentally friendly. Carbon dots (CDs) represent a great potential in novel biomedical applications due to their tunable fluorescence, biocompatibility, and ability to be conjugated with diverse therapeutic materials. There is a growing interest on the exploitation of these properties for drug delivery with enhanced bio-imaging. However, there are limited reports of CD applications for ophthalmic indications. In this review, we focus on the CD potential and the development of translational therapies for ophthalmic diseases. The current review presents better understanding of fabrication of CDs and how it may be useful in delivering anti-bacterial agents, anti-VEGF molecules as well as imaging for ophthalmic applications.

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Section: Key Parameters For Anti-vegf CD Synthesismentioning

confidence: 78%

Carbon Dots Fabrication: Ocular Imaging and Therapeutic Potential

Garner

Vichare

Paulson

et al. 2020

Front. Bioeng. Biotechnol.

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“…The synthesis of CDs and their conjugation with VEGF, as well as their characterization, were performed as reported elsewhere. 17 In brief, citric acid (0.36 g, 1.87 mmol), arginine (0.36 g, 2.07 mmol), and thiourea (0.36 g, 4.72 mmol) were dissolved in 18 mL of DI water. The mixture was transferred to a Teflon-lined autoclave chamber at 180 °C for 22 h. The black precipitate was removed by centrifuging the reaction mixture at 8500g for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have shown similar biocompatibility and lack of cytotoxicity for CDs, free VEGF, and VEGF−CDs for HUVECs. 17 Therefore, cell culture medium with free VEGF was used as a control system. Figure 2C shows that cell viability for the native VEGF group was 100%, whereas the viability of cells in all other groups was >80%.…”

Section: Bioactivity Of Released Vegf−cdsmentioning

confidence: 99%

Composite Microgels for Imaging-Monitored Tracking of the Delivery of Vascular Endothelial Growth Factor to Ischemic Muscles

et al. 2021

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Monitoring the supply of vascular endothelial growth factor (VEGF) to ischemic tissues provides information on its biodistribution and delivery to meet the requirements of therapeutic angiogenesis and tissue engineering applications. We herein report the use of microfluidically generated microgels containing VEGFconjugated fluorescent carbon dots (CDs) (VEGF−CDs), a gelatin− phenol conjugate, and silk fibroin for imaging-monitored tracking of VEGF delivery to ischemic muscles. An in vitro release study and a bioactivity assay indicated that the VEGF−CDs were released in a sustained manner with high bioactivity. The microgels showed a high angiogenesis potential, along with a strong fluorescent signal, for the chicken chorioallantoic membrane and chick embryo. Imaging and studies of therapeutic modalities of the composite microgels indicated their effective localization in ischemic tissues and sustained VEGF release, which resulted in enhanced therapeutic angiogenesis of ischemic muscles. This work reveals the success of using VEGF-loaded composite polymer microgels for efficient and monitored VEGF delivery by intramuscular administration for ischemic disease treatment.

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“…Testing our evolutionary‐based hypothesis will provide the foundation for future development of GDNF‐based treatment options for impaired wound healing and tissue regeneration caused by diabetes, burns and scarring, which largely reflect functional fibroblasts. Integration of GDNF with biodegradable polymers and extracellular matrix meshes, as well as conjugation with carbon dots, may be used to improve delivery and bioactivity in the wound setting 48 . Understanding the regulation of GDNF‐based programs and their integration with other specialized programs to collectively enable human regeneration will be the subject of interesting future studies.…”

Section: Relevance and Perspectivesmentioning

confidence: 99%

GDNF neurotrophic factor signalling determines the fate of dermal fibroblasts in wound‐induced hair neogenesis and skin regeneration

Vishlaghi

Rieger

McGaughey

et al. 2022

Experimental Dermatology

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We propose that GDNF, a glial cell line‐derived neurotrophic factor, can promote hair follicle neogenesis and skin regeneration after wounding by directing the fate of dermal fibroblasts. Our hypothesis is largely based on detailed GDNF and receptor analysis during skin regenerative stages, as well as the induction of GDNF receptors after wounding between the pro‐regenerative spiny mouse (genus Acomys) and its less‐regenerative descendant, the house mouse (Mus musculus). To characterize the GDNF‐target cells, we will conduct a series of lineage‐tracing experiments in conjunction with single‐cell RNA and assay for transposase‐accessible chromatin sequencing experiments. The heterogenetic dynamics of skin regeneration have yet to be fully defined, and this research will help to advance the fields of regenerative medicine and biology. Finally, we believe that stimulating the GDNF signalling pathway in fibroblasts from less‐regenerative animals, such as humans, will promote skin regeneration, morphogenesis and scarless wound healing.

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Carbon Dots Conjugated with Vascular Endothelial Growth Factor for Protein Tracking in Angiogenic Therapy

Cited by 27 publications

References 63 publications

Carbon Dots Fabrication: Ocular Imaging and Therapeutic Potential

Carbon Dots Fabrication: Ocular Imaging and Therapeutic Potential

Composite Microgels for Imaging-Monitored Tracking of the Delivery of Vascular Endothelial Growth Factor to Ischemic Muscles

GDNF neurotrophic factor signalling determines the fate of dermal fibroblasts in wound‐induced hair neogenesis and skin regeneration

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