Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection

Abstract

Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available.

Probing the influence of crosslinkers on the properties, response, and degradation of enzymatic hydrogels for electrochemical glucose biosensing through fluorescence analysis

“…This Schiff base exhibited a relatively significant Stokes shift (65 nm) and a wide emission bandwidth with maximum excitation at 380 nm and maximum emission at 445 nm. 21 Another example of interest is that of chitosan hydrogels crosslinked with genipin. In this case, genipin can fluoresce after the crosslinking process, although the mechanism behind this fluorescence is still limited in understanding.…”

N-Benzylhydroxylamine as a novel synthetic block in “C1N1” embedding reaction via α-C(sp3)–H activation strategy

“…N -Benzylhydroxylamine is a versatile building block that has been widely used in synthetic organic chemistry for the construction of various nitrogen-containing organic frameworks, many of which have demonstrated favorable pharmacological and biological activities. 1 The traditional reaction mode of N -benzylhydroxylamine is to serve as an “N1 source” in the synthesis of amine, ketonitrone and β-lactam derivatives (Scheme 1A-a). 2–4 In addition, only one example has been reported using N -benzylhydroxylamine as an “O1 source” for the construction of branched hydroxylamines (Scheme 1A-b).…”