Fluorescent Cell-Counting Assay of Yellow Fever Virus

Hahon, Nicholas

doi:10.21236/ad0469383

Cited by 2 publications

(5 citation statements)

References 1 publication

(1 reference statement)

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“…stants (K) were 3.4 X 1O-7 cm'/min with centrifugation and 2.8 X 109 cm3/min with stationary incubation. The former attachment rate constant is comparable to those reported for VEE and RVF viruses under similar experimental conditions (5,6).…”

Section: Resultssupporting

confidence: 86%

“…The penetration rate constant (K) of 2.3 X 107 cm8/min was faster than that noted with VEE and RVF viruses (5,6). Because an antiviral serum overlay is employed in this assay to prevent the extracellular spread of virus from infected cells, this information on the rate of virus penetration is highly relevant.…”

Section: Resultsmentioning

confidence: 91%

“…The assay was highly precise, reproducible, and comparable in sensitivity to other established methods of chikungunya virus assessment. The outstanding feature of the method, in common with immunofluorescent assays described for other arboviruses (1,(4)(5)(6), was the ability to assess virus infectivity within 24 hr after inoculation of cell monolayers. The time required to assay chikungunya virus by intracerebral injection of suckling mice or plaque counting was 10 days and from 3 to 4 days, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Hahon¹,

Hankins²

1970

Applied Microbiology

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Hahon¹,

Hankins²

1970

Applied Microbiology

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“…In this current study, the penetration of five representative strains of influenza virus into cells at 35 C proceeded at a linear rate and was complete within 15 min for all virus strains. Similar results were obtained with other animal viruses when attachment and penetration processes were delineated (11)(12)(13). In contrast, to the temperature independency of the attachment reaction, the penetration of influenza virus, as noted with other virus-cell systems (9,12,15,22,27), was temperature dependent.…”

Section: Discussionsupporting

confidence: 82%

“…Although divalent cations, usually calcium and magnesium, have been generally found to be necessary for the electrostatic attachment of bacteriophages (46) and animal viruses to host cells (2, 23,26), there are numerous exceptions (11)(12)(13)21). Attachment of influenza virus to cells proved to be another exception.…”

Section: Discussionmentioning

confidence: 99%

Cell Attachment and Penetration by Influenza Virus

Hahon¹,

Booth²,

Eckert³

1973

Infect Immun

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Attachment and penetration of influenza virus into clone 1-5C-4 cells were quantitatively determined by the immunofluorescent cell-counting assay. Aided by centrifugal force, more than 95% of virus inocula of five representative influenza virus strains (Ao/PR8, Al/Ann Arbor, A2/Japan, B/Lee, B/Great Lakes) were attached to cells at a linear rate within 10 min, in contrast to approximately 35% after stationary incubation at 35 C for 2 h. By the former procedure, a proportionality between the number of infected cells and volume of inoculum was revealed which was not evident when stationary incubation was employed. Maximal binding of virus to cells occurred at 0.2 M NaCl. The salt requirement, added to evidence of pH dependence and temperature independence, indicated that the initial virus-cell union involved electrostatic forces. Virus penetration into cells, measured by the insensitivity of virus-cell complexes to antiviral serum, was linear and complete within 15 min at 35 C for all five virus strains tested. Maximal virus penetration occurred at 0.1 to 0.2 M NaCl; the process was pHand temperaturedependent. Both virus attachment and penetration processes were partially inhibited in the presence of diethylaminoethyl-dextran. circular cover slips (15 mm diameter) inserted in 341 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Fluorescent Cell-Counting Assay of Yellow Fever Virus

Cited by 2 publications

References 1 publication

Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Cell Attachment and Penetration by Influenza Virus

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