Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Hahon, Nicholas; Hankins, William A.

doi:10.1128/aem.19.2.224-231.1970

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…In this current study, the penetration of five representative strains of influenza virus into cells at 35 C proceeded at a linear rate and was complete within 15 min for all virus strains. Similar results were obtained with other animal viruses when attachment and penetration processes were delineated (11)(12)(13). In contrast, to the temperature independency of the attachment reaction, the penetration of influenza virus, as noted with other virus-cell systems (9,12,15,22,27), was temperature dependent.…”

Section: Discussionsupporting

confidence: 81%

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Cell Attachment and Penetration by Influenza Virus

Hahon¹,

Booth²,

Eckert³

1973

Infect Immun

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Attachment and penetration of influenza virus into clone 1-5C-4 cells were quantitatively determined by the immunofluorescent cell-counting assay. Aided by centrifugal force, more than 95% of virus inocula of five representative influenza virus strains (Ao/PR8, Al/Ann Arbor, A2/Japan, B/Lee, B/Great Lakes) were attached to cells at a linear rate within 10 min, in contrast to approximately 35% after stationary incubation at 35 C for 2 h. By the former procedure, a proportionality between the number of infected cells and volume of inoculum was revealed which was not evident when stationary incubation was employed. Maximal binding of virus to cells occurred at 0.2 M NaCl. The salt requirement, added to evidence of pH dependence and temperature independence, indicated that the initial virus-cell union involved electrostatic forces. Virus penetration into cells, measured by the insensitivity of virus-cell complexes to antiviral serum, was linear and complete within 15 min at 35 C for all five virus strains tested. Maximal virus penetration occurred at 0.1 to 0.2 M NaCl; the process was pHand temperaturedependent. Both virus attachment and penetration processes were partially inhibited in the presence of diethylaminoethyl-dextran. circular cover slips (15 mm diameter) inserted in 341 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Discussionsupporting

confidence: 81%

“…Although divalent cations, usually calcium and magnesium, have been generally found to be necessary for the electrostatic attachment of bacteriophages (46) and animal viruses to host cells (2, 23,26), there are numerous exceptions (11)(12)(13)21). Attachment of influenza virus to cells proved to be another exception.…”

Section: Discussionmentioning

confidence: 99%

Cell Attachment and Penetration by Influenza Virus

Hahon¹,

Booth²,

Eckert³

1973

Infect Immun

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Virological and immunofluorescent studies on the multiplication of sindbis virus in L cells

Veckenstedt¹,

Wagner²

1973

Archiv f Virusforschung

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Infection of young adult mice with dengue virus type 2

Boonpucknavig

Vuttiviroj

Boonpucknavig

1981

Transactions of the Royal Society of Tropical Medicine and Hygi

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Young adult female mice, five to six weeks old, were injected intraperitoneally with 2.5 x 10(6.3) LD50 of dengue-2 virus, New Guinea C strain. The mice were killed on day 1, 2, 3, 4, 5, 6, 7, 10, 14, 21, 28, and 35 respectively. By means of the immunofluorescent antibody technique, viral antigen appeared as irregular granules in the reticuloendothelial cells of liver, lymph nodes and spleen of infected mice on the first day after inoculation and then diminished. From the fifth to sixth day of infection dengue antigen appeared again as homogeneous staining in the cytoplasm of single or groups of mononuclear cells in the lymphatic sinuses only. Later, by the third week of infection, dengue antigen could be seen in the mononuclear cells located in the marginal zone of lymphoid follicle of the spleen, the pattern of staining changing to bright spherical granules. At the same time, the deposition of immune complexes (composed of dengue antigen, mouse gamma and beta 1C globulin) could be seen in the renal glomeruli of infected mice. Serum antibody to dengue virus was found at low levels, being maximal on the 14th day after infection. Dengue virus was not isolated from the sera or from the infected organs. Granulomatous inflammation developed in lymph nodes and liver of mice infected with dengue virus and in mice inoculated with normal mouse brain suspension. Proliferative glomerular lesion was observed on day 14 after inoculation without definite abnormal urine findings.

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Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Cited by 6 publications

References 7 publications

Cell Attachment and Penetration by Influenza Virus

Cell Attachment and Penetration by Influenza Virus

Virological and immunofluorescent studies on the multiplication of sindbis virus in L cells

Infection of young adult mice with dengue virus type 2

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