Virological and immunofluorescent studies on the multiplication of sindbis virus in L cells

Veckenstedt, A.; Wagner, M.

doi:10.1007/bf01270834

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…For host range variants, both differences in adsorption efficiency and intracellular replication in the new host will determine how many serial passages are required before the variant dominates the virus population. One Sindbis variant previously described that affected mouse cells required many passages on mouse L cells before it became the major type of virus in the stock (22). In contrast, the variant isolated in our work appeared very quickly, after only three or four serial passages.…”

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confidence: 51%

Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells

Symington¹,

Schlesinger²

1975

J Virol

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A variant of Sindbis virus has been isolated by growing a stock of virus, previously passaged on chicken embryo cells, in mouse plasmacytoma (MOPC 31a) cells in suspension culture. An indirect immunofluorescence test and infective center assay showed that only a small fraction of cells could be infected by the stock wild-type virus, but that the population of virus accumulating after a few passages on the mouse cells had host-range properties distinct from the stock virus. The mouse-passaged virus retained its virulence for the original host and back-passaging of this virus on chicken cells did not change its newly acquired properties. Thus, this variant appears to be a genetically distinct form of Sindbis that adsorbs to and grows much better than the stock virus on several types of mouse cells including cultures of mouse macrophages.

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confidence: 51%

Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells

Symington¹,

Schlesinger²

1975

J Virol

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“…L cells grown in Eagle's minimum essential medium, supplemented with 10% of calf serum and antibiotics, were infected with about 10 PFU/cell of an L cell-adapted strain of Sindbis virus in its 82nd passage [4], To prove the identity of the crystalline-arranged particles with Sindbis virus antigens, the indirect immunoferritin technique was used. Cell suspen sions, after washing in Tris buffer, were fixed in 2.7% buffered formaldehyde for 30 min, pelleted by centrifugation at low speed, washed, quick-frozen in a COz-ethanol bath, rapidly thawed and suspended in Sindbis virus anti serum from guinea pigs [4] for 1 h at about 20'.…”

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confidence: 99%

“…Cell suspen sions, after washing in Tris buffer, were fixed in 2.7% buffered formaldehyde for 30 min, pelleted by centrifugation at low speed, washed, quick-frozen in a COz-ethanol bath, rapidly thawed and suspended in Sindbis virus anti serum from guinea pigs [4] for 1 h at about 20'. The washed cells were treated with ferritin-labelled anti-guinea pig IgG [5] in the same manner.…”

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confidence: 99%