Fluorescent biosensor based on magnetic cross-linking enzyme aggregates/CdTe quantum dots for the detection of H<sub>2</sub>O<sub>2</sub>-bioprecursors

Chen, Guoning; Hu, Qianqian; Shu, Hua; Wang, Lu; Cui, Xia; Han, Jili; Bashir, Kamran; Luo, Zhimin; Chang, Chun Ming; Fu, Qiang

doi:10.1039/d0nj03761c

Cited by 6 publications

(4 citation statements)

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“…The activity of glucose oxidase was measured using the methods of a previous report with some modification [16]. Glucose causes a rapid release of H 2 O 2 by the catalytic action of glucose oxidase.…”

Section: Activity Assaymentioning

confidence: 99%

“…Recently, various sensing methods for H 2 O 2 have been employed to detect glucose because glucose can cause a rapid release of H 2 O 2 by the catalytic action of its oxidases [14][15][16]. However, some inherent deficiencies of natural enzymes, such as low operational stability, low anti-interference to circumstances, and poor recyclability, limit their practical applications.…”

Section: Introductionmentioning

confidence: 99%

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Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

et al. 2022

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Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system. The encapsulated enzyme was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and a vibrating sample magnetometer. Finally, a colorimetric sensing method was developed for the detection of glucose in urine samples based on the encapsulated glucose oxidase and a hydrogen peroxide test strip. The method exhibited a good linear performance in the concentration range of 20~160 μg mL−1 and good recoveries ranging from 94.3 to 118.0%. This work proves that the self-assembly method could be employed to encapsulate glucose oxidase into silica-coated magnetic particles. The developed colorimetric sensing method shows high sensitivity, which will provide a promising tool for the detection of glucose and the monitoring of diabetes.

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Section: Activity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

et al. 2022

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“…Preparation of CdSe QDs-Au NPs-NAR-Ab 2 . Based on a previous work, 30 the preparation of CdSe QDs was improved (Section S5 in the Supporting Information). Also, after the reaction, the product was cooled to room temperature and stored in a 4 °C refrigerator.…”

Section: ■ Introductionmentioning

confidence: 99%

High-Efficiency CdSe Quantum Dots/Fe₃O₄@MoS₂/S₂O₈^2– Electrochemiluminescence System Based on a Microfluidic Analysis Platform for the Sensitive Detection of Neuron-Specific Enolase

Feng

Song

et al. 2022

Anal. Chem.

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In this work, based on electrochemiluminescence (ECL) technology and self-assembled portable disease detection chips, a bioactivity-maintained sensing platform was developed for the quantitative detection of neuron-specific enolase. First, we prepared Fe3O4@MoS2 nanocomposites as an efficient catalyst to accelerate the reduction of persulfate (S2O8 2–). Specifically, abundant sulfate radicals (SO4 •–) were generated because of cyclic conversion between Fe2+ and Fe3+. Meanwhile, MoS2 nanoflowers with a high specific surface area could not only load more Fe3O4 but also solve its agglomeration problem, which greatly improved the catalytic efficiency. Moreover, a biosensor chip was constructed by standard lithography processes for disease detection, which had good sensitivity and portability. According to the above strategies, the developed portable sensing platform played the part of promoting the practical application of bioanalysis in early tumor screening and clinical diagnosis. In addition, we developed a short peptide ligand (NARKFYKG, NAR) to avoid the occupation of antigen binding sites by specifically connecting to Fc fragments in antibodies. Thus, the binding efficiency of antibodies and the activity of biosensors were improved due to the introduction of NAR.

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“…Nowadays, enzyme immobilization has been found to greatly improve the stability of enzymes by preventing aggregation, autolysis, and proteolysis. 11,12 Carriers used in enzyme immobilization can affect the stability and the microenvironment of the enzyme, which in turn will affect the catalytic efficiency of the immobilized enzyme. Significant efforts have been devoted to developing carriers for this technology.…”

Section: Introductionmentioning

confidence: 99%

Nanoreactor of sarcosine oxidase-embedded ZIFs activates fluorescent response for diagnosis of prostate cancer

Chen

Wang

et al. 2022

New J. Chem.

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Fluorescent biosensor based on magnetic cross-linking enzyme aggregates/CdTe quantum dots for the detection of H₂O₂-bioprecursors

Abstract: The sensing method for hydrogen peroxide could be employed to detect the "H2O2-bioprecursor" in the presence of oxidase. However, the sensitivity of the conventional method is limited, and the catalytic...

Cited by 6 publications

References 56 publications

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

High-Efficiency CdSe Quantum Dots/Fe₃O₄@MoS₂/S₂O₈^2– Electrochemiluminescence System Based on a Microfluidic Analysis Platform for the Sensitive Detection of Neuron-Specific Enolase

Nanoreactor of sarcosine oxidase-embedded ZIFs activates fluorescent response for diagnosis of prostate cancer

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Fluorescent biosensor based on magnetic cross-linking enzyme aggregates/CdTe quantum dots for the detection of H2O2-bioprecursors

Abstract: The sensing method for hydrogen peroxide could be employed to detect the "H2O2-bioprecursor" in the presence of oxidase. However, the sensitivity of the conventional method is limited, and the catalytic...

Cited by 6 publications

References 56 publications

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

High-Efficiency CdSe Quantum Dots/Fe3O4@MoS2/S2O82– Electrochemiluminescence System Based on a Microfluidic Analysis Platform for the Sensitive Detection of Neuron-Specific Enolase

Nanoreactor of sarcosine oxidase-embedded ZIFs activates fluorescent response for diagnosis of prostate cancer

Contact Info

Product

Resources

About

Fluorescent biosensor based on magnetic cross-linking enzyme aggregates/CdTe quantum dots for the detection of H₂O₂-bioprecursors

High-Efficiency CdSe Quantum Dots/Fe₃O₄@MoS₂/S₂O₈^2– Electrochemiluminescence System Based on a Microfluidic Analysis Platform for the Sensitive Detection of Neuron-Specific Enolase