High-Efficiency CdSe Quantum Dots/Fe₃O₄@MoS₂/S₂O₈^2– Electrochemiluminescence System Based on a Microfluidic Analysis Platform for the Sensitive Detection of Neuron-Specific Enolase

Abstract

In this work, based on electrochemiluminescence (ECL) technology and self-assembled portable disease detection chips, a bioactivity-maintained sensing platform was developed for the quantitative detection of neuron-specific enolase. First, we prepared Fe3O4@MoS2 nanocomposites as an efficient catalyst to accelerate the reduction of persulfate (S2O8 2–). Specifically, abundant sulfate radicals (SO4 •–) were generated because of cyclic conversion between Fe2+ and Fe3+. Meanwhile, MoS2 nanoflowers with a high specific surface area could not only load more Fe3O4 but also solve its agglomeration problem, which greatly improved the catalytic efficiency. Moreover, a biosensor chip was constructed by standard lithography processes for disease detection, which had good sensitivity and portability. According to the above strategies, the developed portable sensing platform played the part of promoting the practical application of bioanalysis in early tumor screening and clinical diagnosis. In addition, we developed a short peptide ligand (NARKFYKG, NAR) to avoid the occupation of antigen binding sites by specifically connecting to Fc fragments in antibodies. Thus, the binding efficiency of antibodies and the activity of biosensors were improved due to the introduction of NAR.

Cocatalyst Modification of Rutile TiO2 Nanorods for Photocatalytic Water Oxidation

“…These findings suggest that the photogenerated electron in the conduction band of NR(GA) reduces Fe 3+ to Fe 2+ (eqs and ), which reduces S 2 O 8 2– to SO 4 ·– (eq ). Equation is known as a Fenton-like reaction and has been extensively used for various applications such as wastewater treatment. − Fe 3 + ‐ NR false( GA false) + h ν → Fe 3 + ‐ NR false( GA false) false( normalh + + normale − false) Fe 3 + ‐ NR false( GA false) false( normalh + + normale − false) → Fe 2 + + NR false( GA false) false( normalh + false) Fe 2 + + normalS 2 normalO 8 2 − → Fe 3 + + SO 4 · − + SO …”

Rapid and sensitive electrochemiluminescence detection using easily fabricated sensor with an integrated two-electrode system

“…With the continuous development of microfluidic technology and microelectro-mechanical systems (MEMS) technology, many micro-electrode systems have been integrated into microfluidic chips for the sensitive detection of biomolecules. 20–22 Satienperakul's group developed a simple 3D-printed platform with a three-electrode system, which was used to detect sibutramine in dietary supplements, demonstrating the broad potential for micro-electrode systems in ECL-based biosensor applications. 23 Although current microchips utilize a three-electrode system to achieve ECL reaction, three-electrode system increases the complexity of multi-detection applications on a single microchip.…”

Potential-Resolved Electrochemiluminescence Multiplex Immunoassay for Florfenicol and Chloramphenicol in a Single Sample

“…As shown in Scheme A, because of the good water solubility of SnS 2 QDs, they were first coupled with polyethylenimine (PEI) to obtain SnS 2 QDs-PEI . Remarkably, Au nanoparticles-modified MoS 2 (Au-MoS 2 ) has a large specific surface area, biocompatibility, electrocatalytic activity, and good electrical conductivity. , Accordingly, to enhance the ECL cathodoluminescence intensity, Au-MoS 2 was chosen to bind to SnS 2 QDs-PEI via Au–N bonding to obtain the SnS 2 QDs-PEI-Au-MoS 2 complexes. Then, it was bound to CAP antibody via Au–N to access the ECL cathodoluminescence probe.…”