Fluorescent antigen-antibody reactions in Coxsackie and ECHO enteroviruses

Shaw, Eugene D.; Newton, A. P.; Powell, Albert W.; Friday, Charles J.

doi:10.1016/0042-6822(61)90239-2

Cited by 23 publications

(8 citation statements)

References 2 publications

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“…(e) Influenza A(FB0013/68) in MEK cells versus antiinfluenza A(PR8) serum; 42 hr postinoculation. (f) Influenza B(GL18/66) in RK cells versus antirespiratory viral serum pool; 18 hr postinoculation.…”

Section: Resultsmentioning

confidence: 99%

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Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

Stevens¹,

Watkins²

1969

Applied Microbiology

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The indirect fluorescent-antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in 18 to 45 hr after inoculation of slide microtissue culture.

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Section: Resultsmentioning

confidence: 99%

“…One approach to rapid identification of viruses involves the use of pooled specific antisera (13,18,19) or, alternatively, widely reactive, groupspecific antisera (15) employed in the indirect fluorescent-antibody test (IFAT; 23). However, in virology, use of the IFAT has been hampered by serious problems of nonspecific staining.…”

mentioning

confidence: 99%

Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

Stevens¹,

Watkins²

1969

Applied Microbiology

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“…Hemagglutination inhibition systems are limited by the fact that many strains of Coxsackie B virus obtained directly from clinical specimens do not cause measurable hemagglutination [ 11,141 . Immunofluorescence systems have not attained widespread usage, possibly due to the difficulty of making objective measurements in the face of cross-reacting antisera [16]. CF systems can be devised to distinguish Coxsackie B antigens but are limited by the relative insensitivity of CF systems [5, 111.…”

Section: Clinical Specimensmentioning

confidence: 99%

“…While the Coxsackie B viruses can be identified by [9,111 . While other methods of identifying Coxsackie viruses in tissue cultures such as immunofluorescence [16] , complement fixation (CF) [5] and hemagglutination inhibition [14] have been described, these methods have not attained widespread usage in clinical diagnostic laboratories.…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐Linked immunosorbent assay for the detection and identification of coxsackie b antigen in tissue cultures and clinical specimens

Yolken

Torsch

1980

Journal of Medical Virology

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An enzyme-linked immunosorbent assay (ELISA) has been developed for the identification of Coxsackie B antigens. This assay was capable of identifying and distinguishing all six Coxsackie B serotypes at concentrations one hundredfold to ten thousandfold less than could be detected by complement fixation (CF) systems. In addition, the Coxsackie B ELISA correctly identified the presence of Coxsackie B antigen in 19 of 21 tissue culture fluids and five of nine rectal swab specimens. Two additional rectal swab specimens reacted with Coxsackie B antisera but could not be conclusively serotyped. Tissue culture fluids and rectal swab specimens containing other viruses such as ECHO virus, Coxsackie virus A, rhinovirus, rotavirus and Norwalk virus were consistently negative in the assay. The Coxsackie B ELISA offers potential for the rapid identification of Coxsackie B antigens in clinical specimens and tissue culture systems.

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“…Our findings suggest that the IF technique can be applied for rapid diagnosis of AHC infection. Such a procedure has been shown to be practical for detecting enterovirus-infected cells in spinal fluid and for rapid typing of enteroviruses [8,9],…”

mentioning

confidence: 99%

Rapid Immunofluorescence Diagnosis of Acute Hemorrhagic Conjunctivitis Caused by Enterovirus 70

1983

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During 1981–1982, epidemics of acute hemorrhagic conjunctivitis (AHC) occurred in and around Chandigarh, India. Conjunctival swabs were taken from AHC cases and investigated by the indirect immunofluorescence method both in Chandigarh and in Houston. By using reference antiserum, both laboratories proved enterovirus type 70 to be the causative agent of the epidemics. The immunofluorescence test was evaluated as a rapid method for the diagnosis of AHC.

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Fluorescent antigen-antibody reactions in Coxsackie and ECHO enteroviruses

Cited by 23 publications

References 2 publications

Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

Enzyme‐Linked immunosorbent assay for the detection and identification of coxsackie b antigen in tissue cultures and clinical specimens

Rapid Immunofluorescence Diagnosis of Acute Hemorrhagic Conjunctivitis Caused by Enterovirus 70

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