Fluorescence transients in chloroplasts of Chara corallina cells during transmission of photoinduced signal with the streaming cytoplasm

Булычев, А. А.; Alova, A.; Rubin, A. B.

doi:10.1134/s1021443712060039

Cited by 11 publications

(2 citation statements)

References 23 publications

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“…It is known that VP generation is connected with transient H + -ATPase inactivation and proton influx (Stahlberg et al, 2006 ; Sukhov et al, 2013b ), which changes intra- and extracellular pH (Grams et al, 2009 ; Sukhov et al, 2014a ). However, decreased intracellular pH can suppress PSII photosynthetic activity and induces NPQ (Grams et al, 2009 ; Bulychev et al, 2013a , b ; Sukhov et al, 2013a , 2014a ). Taking into account these facts, it can be proposed that VP → H + influx → inactivation of photosynthetic dark stage and NPQ growth → EF(PSII) decrease → ϕ ND increase → EF(C) growth.…”

Section: Discussionmentioning

confidence: 99%

Variation potential influence on photosynthetic cyclic electron flow in pea

et al. 2015

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Cyclic electron flow is an important component of the total photosynthetic electron flow and participates in adaptation to the action of stressors. Local leaf stimulation induces electrical signals, including variation potential (VP), which inactivate photosynthesis; however, their influence on cyclic electron flow has not been investigated. The aim of this study was to investigate VP's influence on cyclic electron flow in pea (Pisum sativum L.). VP was induced in pea seedling leaves by local heating and measured in an adjacent, undamaged leaf by extracellular electrodes. CO2 assimilation was measured using a portable gas exchange measuring system. Photosystem I and II parameters were investigated using a measuring system for simultaneous assessment of P700 oxidation and chlorophyll fluorescence. Heating-induced VP reduced CO2 assimilation and electron flow through photosystem II. In response, cyclic electron flow rapidly decreased and subsequently slowly increased. Slow increases in cyclic flow were caused by decreased electron flow through photosystem II, which was mainly connected with VP-induced photosynthetic dark stage inactivation. However, direct influence by VP on photosystem I also participated in activation of cyclic electron flow. Thus, VP, induced by local leaf-heating, activated cyclic electron flow in undamaged leaves. This response was similar to photosynthetic changes observed under the direct action of stressors. Possible mechanisms of VP's influence on cyclic flow were discussed.

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Section: Discussionmentioning

confidence: 99%

Variation potential influence on photosynthetic cyclic electron flow in pea

et al. 2015

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“…(iv) Artificial inactivation of H + -ATP-ase [ 166 ] or induction of a proton influx [ 134 ] causes photosynthetic changes which are similar to the fast changes induced by ESs. (v) The artificial decrease in the pH in medium for chloroplasts [ 134 , 150 , 172 ] or in perfused cells [ 173 ] induces photosynthetic changes which are similar to the fast changes induced by ESs. Results showed [ 8 , 35 ] that there are at least two mechanisms of these ESs’ effects on photosynthetic processes: the increase in the apoplastic pH, which suppresses CO 2 flux into cells (probably through changes in the CO 2 /HCO 3 - ratio [ 172 ] and modifications of activity of aquaporins transmitting CO 2 [ 128 ]), and the decrease in the cytoplasmic pH and thereby the pH in the stroma and lumen, which directly affect photosynthetic light reactions (e.g., through stimulation of NPQ [ 174 ]).…”

Section: Physiological Roles Of Electrical Signalsmentioning

confidence: 99%

Electrical Signals, Plant Tolerance to Actions of Stressors, and Programmed Cell Death: Is Interaction Possible?

Sukhova

Sukhov

2021

Plants

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In environmental conditions, plants are affected by abiotic and biotic stressors which can be heterogenous. This means that the systemic plant adaptive responses on their actions require long-distance stress signals including electrical signals (ESs). ESs are based on transient changes in the activities of ion channels and H+-ATP-ase in the plasma membrane. They influence numerous physiological processes, including gene expression, phytohormone synthesis, photosynthesis, respiration, phloem mass flow, ATP content, and many others. It is considered that these changes increase plant tolerance to the action of stressors; the effect can be related to stimulation of damages of specific molecular structures. In this review, we hypothesize that programmed cell death (PCD) in plant cells can be interconnected with ESs. There are the following points supporting this hypothesis. (i) Propagation of ESs can be related to ROS waves; these waves are a probable mechanism of PCD initiation. (ii) ESs induce the inactivation of photosynthetic dark reactions and activation of respiration. Both responses can also produce ROS and, probably, induce PCD. (iii) ESs stimulate the synthesis of stress phytohormones (e.g., jasmonic acid, salicylic acid, and ethylene) which are known to contribute to the induction of PCD. (iv) Generation of ESs accompanies K+ efflux from the cytoplasm that is also a mechanism of induction of PCD. Our review argues for the possibility of PCD induction by electrical signals and shows some directions of future investigations in the field.

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Proton flows across the plasma membrane in microperforated characean internodes: tonoplast injury and involvement of cytoplasmic streaming

Булычев

Комарова

2014

Protoplasma

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Microperforation of characean cell wall with a glass micropipette in the absence of the tonoplast impalement was found to cause rapid alkalinization of the apoplast by 2-3 pH units, which may rigidify the cell wall structure, thus protecting the cell from further injury. A similar but a deeper insertion of a microneedle, associated with piercing the tonoplast and with an action potential generation, led to a considerable delay in the apoplast alkalinization without affecting the amplitude of the eventual increase in pH. The retardation by the mechanically elicited action potential of the incision-mediated pH transients in the apoplast contrasted sharply to the enhancement of these pH transients by the action potential triggered electrically before the microperforation. Hence, the delay of the apoplast alkalinization was not related to basic ionic mechanisms of plant action potentials. Measurements of the vacuolar pH after mechanical elicitation of an action potential indicate that the tonoplast piercing was accompanied by leakage of protons from the vacuole into the cytoplasm, which may strongly acidify the cytoplasm around the wounded area, thus collapsing the driving force for H(+) influx from the medium into the cytoplasm. The lag period preceding the onset of external alkalinization was found linearly related to the duration of temporal cessation of cytoplasmic streaming. The results suggest that the delayed alkalinization of the apoplast in the region of tonoplast wounding reflects the localized recovery of the proton motive force across the plasmalemma during replacement of the acidic cytoplasm with fresh portions of unimpaired cytoplasm upon restoration of cytoplasmic streaming.

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Fluorescence transients in chloroplasts of Chara corallina cells during transmission of photoinduced signal with the streaming cytoplasm

Cited by 11 publications

References 23 publications

Variation potential influence on photosynthetic cyclic electron flow in pea

Variation potential influence on photosynthetic cyclic electron flow in pea

Electrical Signals, Plant Tolerance to Actions of Stressors, and Programmed Cell Death: Is Interaction Possible?

Proton flows across the plasma membrane in microperforated characean internodes: tonoplast injury and involvement of cytoplasmic streaming

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