Fluorescence Studies of the Calcium Binding to Whiting (Gadus merlangus) Parvalbumin

Abstract

The calcium binding by parvalbumin of whiting (Gadus merlangus) has been studied using tryptophanyl fluorescence characteristics. Titration of Ca2+-free parvalbumin with Ca2+ leads to a very pronounced blue shift, narrowing and intensification of the fluorescence spectrum. These spectral changes proceed in two stages reflecting the existence of at least three forms which can be interpreted as (a) the protein without Ca2+, (b) with one Ca2+ and (c) with two bound Ca2+ ions/molecule. The fluorescence of these forms has been identified and the fluorescence spectra measured at varied Ca2+ concentrations were resolved into three components corresponding to these spectral forms.

Partially Folded Conformations in the Folding Pathway of Bovine Carbonic Anhydrase II: A Fluorescence Spectroscopic Analysis

“…[21] It has been shown that such an approach is extremely sensitive to the accumulation of any intermediate state. [21,22] Figure 3 clearly shows that the phase diagram plotted for the GdmCl-induced unfolding of BCA II consists of three linear parts, corresponding to 0 ± 0.1, 0.1 ± 1.0 and 1.0 ± 6.0 M GdmCl concentration. This reflects the existence of three independent transitions: N 6 I GdmCl any other structural parameter takes place.…”

Influences of cationic, anionic, and nonionic surfactants on alkaline-induced intermediate of bovine serum albumin

“…The 'phase diagram' method, initially described by Burstein for the analysis of fluorescence data, is extremely sensitive for the detection of any intermediate states [21,22]. The essence of this method is to build up the diagram of I ( 1 ) versus I ( 2 ), where I ( 1 ) and I ( 2 ) are the fluorescence intensity values measured on wavelengths 1 and 2 under different experimental conditions when a protein is undergoing structural transformations.…”

Effect of SNPs on Creatine Kinase Structure and Function: Identifying Potential Molecular Mechanisms for Possible Creatine Kinase Deficiency Diseases

“…Fluorescence data were analyzed according to the phase diagram method, which is sensitive for detecting any intermediate state [29], [30]. The essential point of this method is to build up a diagram of I λ1 versus I λ2 , where the I λ1 and I λ2 are the fluorescence intensities at two different emission wavelengths.…”