Fluorescence resonance energy transfer (FRET)-based technique for tracking of endophytic bacteria in rice roots

Banik, Avishek; Mukhopadhaya, Subhra Kanti; Sahana, Animesh; Dangar, Tushar Kanti

doi:10.1007/s00374-015-1064-6

Cited by 18 publications

(7 citation statements)

References 25 publications

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“…Tracking helps to investigate the potential of inoculated microbes because PGPR is based on their persistence in the soil. Tracking bio-inoculants in the soil has been performed using different methods, including dilution plating and microscopy [49,50]. However, such methods can be laborious, time-consuming, and limited to sterile conditions [51].…”

Section: Discussionmentioning

confidence: 99%

Response of Soil Bacterial Community and Pepper Plant Growth to Application of Bacillus thuringiensis KNU-07

Tagele

Pham

et al. 2020

Agronomy

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Many Bacillus species are among the plant growth-promoting rhizobacteria (PGPR) that promote the growth of many different plant species. This study aimed to investigate the effects of Bacillus thuringiensis KNU-07 on the growth of pepper plants and the soil microbiota. We also designed primers specific for the strain KNU-07 to monitor the population in pepper-cultivated soil. Accordingly, a strain-specific primer pair was designed using a database constructed from 16,160 complete bacterial genomes. We employed quantitative PCR (qPCR) to track the abundance of the strain KNU-07 introduced into pepper-cultivated soil using the strain-specific primers. Our study revealed that the strain was found to possess plant growth-promoting (PGP) activities, and it promoted the growth of pepper plants. The soil bacterial community structure due to the application of the PGPR strain was significantly changed after six weeks post-inoculation. In addition, based on qPCR analysis, the population of the introduced strain declined over time. In this study, application of a PGPR strain increased the growth of pepper plants and changed the soil bacterial community structure. The successful results of monitoring of a bacterial strain’s population using a single strain-specific primer pair can provide important information about the quantification of bio-inoculants under non-sterile soil conditions.

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Section: Discussionmentioning

confidence: 99%

Response of Soil Bacterial Community and Pepper Plant Growth to Application of Bacillus thuringiensis KNU-07

Tagele

Pham

et al. 2020

Agronomy

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“…Tomato seeds ( Solanum lycopersicum var. cerasiforme ) were carefully de-husked without damaging the embryo and surface sterilized as described by Banik et al (2016) . Briefly, seeds were treated with 2% sodium hypochlorite (5 min) followed by washing with sterile distilled water, then seeds were treated with 75% ethanol (5 min), washed again with sterile water and treated with 30% hydrogen peroxide (2 min) as suggested by Amarasinghe et al (2018) ; finally, they were carefully rinsed ten times with sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

Romano

Ventorino

Ambrosino³

et al. 2020

Front. Microbiol.

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The use of beneficial microbes as inoculants able to improve fitness, growth and health of plants also in stress conditions is an attractive low-cost and eco-friendly alternative strategy to harmful chemical inputs. Thirteen potential plant growth-promoting bacteria were isolated from the rhizosphere of wheat plants cultivated under drought stress and nitrogen deficiency. Among these, the two isolates TL8 and TL13 showed multiple plant growth promotion activities as production of indole-3-acetic acid (IAA), siderophores, ammonia, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase production, the ability to solubilize phosphate as well as exerted antimicrobial activity against plant pathogens as Botrytis spp. and Phytophthora spp. The two selected strains were identified as Kosakonia pseudosacchari by sequencing of 16S rRNA gene. They resulted also tolerant to abiotic stress and were able to efficiently colonize plant roots as observed in vitro assay under fluorescence microscope. Based on the best PGP properties, the strain K. pseudosacchari TL13 was selected to develop a new microbial based formulate. A sustainable and environmentally friendly process for inoculant production was developed using agro-industrial by-products for microbial growth. Moreover, the application of K. pseudosacchari TL13- based formulates in pot experiment improved growth performance of maize plants.

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“…Main targets for enhancing beneficial plant–microbial interactions include quorum sensing, motility of bacterial species, biofilm formation, and different signalling pathways associated with plant–microbial interactions (Farrar et al 2014 ; Banik et al 2016b ). Microbial cells in biofilm interact via numerous signalling substances to modify their operational mechanisms via coordinated release of antibiotics and toxins, as well as in gene expression modulation via quorum sensing (Rayanoothala et al 2021 ).…”

Section: Flavonoids In Non-rhizobial Plant–bacterial Interactionsmentioning

confidence: 99%

Flavonoid mediated selective cross-talk between plants and beneficial soil microbiome

et al. 2022

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Plants generate a wide variety of organic components during their different growth phases. The majority of those compounds have been classified as primary and secondary metabolites. Secondary metabolites are essential in plants’ adaptation to new changing environments and in managing several biotic and abiotic stress. It also invests some of its photosynthesized carbon as secondary metabolites to establish a mutual relationship with soil microorganisms in that specific niche. As soil harbors both pathogenic and beneficial microorganisms, it is essential to identify some specific metabolites that can discriminate beneficial and pathogenic ones. Thus, a detailed understanding of metabolite’s architectures that interact with beneficial microorganisms could open a new horizon of ecology and agricultural research. Flavonoids are used as classic examples of secondary metabolites in this study to demonstrate recent developments in understanding and realizing how these valuable metabolites can be controlled at different levels. Most of the research was focused on plant flavonoids, which shield the host plant against competitors or predators, as well as having other ecological implications. Thus, in the present review, our goal is to cover a wide range of functional and signalling activities of secondary metabolites especially, flavonoids mediated selective cross-talk between plant and its beneficial soil microbiome. Here, we have summarized recent advances in understanding the interactions between plant species and their rhizosphere microbiomes through root exudates (flavonoids), with a focus on how these exudates facilitate rhizospheric associations. Supplementary Information The online version contains supplementary material available at 10.1007/s11101-022-09806-3.

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Fluorescence resonance energy transfer (FRET)-based technique for tracking of endophytic bacteria in rice roots

Cited by 18 publications

References 25 publications

Response of Soil Bacterial Community and Pepper Plant Growth to Application of Bacillus thuringiensis KNU-07

Response of Soil Bacterial Community and Pepper Plant Growth to Application of Bacillus thuringiensis KNU-07

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

Flavonoid mediated selective cross-talk between plants and beneficial soil microbiome

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