Fluorescence quenching of newly synthesized biologically active coumarin derivative by aniline in binary solvent mixtures

Evale, Basavaraj G.; Hanagodimath, S.M.

doi:10.1016/j.jlumin.2009.05.017

Cited by 22 publications

(5 citation statements)

References 32 publications

(55 reference statements)

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“…It was observed that the K SV values increased with change in dielectric constant ( ε ) in a non‐linear manner, which can be explained by the greater charge‐transfer characteristics of the exciplex in the polar solvent . The higher values for K SV in polar solvents such as DMSO, DMF, ACN compared with non‐polar solvents, namely TOL and DXN, may also be explained by the greater charge‐transfer characteristics of the excited complex in the polar solvent . Similar results have been incorporated by other researchers in some fluorophore quencher systems …”

Section: Resultssupporting

confidence: 76%

Bimolecular fluorescence quenching reactions of the biologically active coumarin composite 2‐acetyl‐3H‐benzo[f]chromen‐3‐one in different solvents

Koppal

Melavanki

Kusanur³

et al. 2018

Luminescence

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A study on fluorescence quenching was carried out for the coumarin derivative 2-acetyl-3H-benzo[f]chromen-3-one (2AHBC) with aniline at room temperature. Efficient fluorescence quenching was observed and Stern-Volmer (S-V) plots showed upward curves from linearity in all solvents of different polarities. For the solute 2AHBC, ground state complex formation does not hold in our study. The kinetic distance (r) value was found to be greater than the encounter distance (R) and indicated that the quenching reaction was held within the sphere of action. Diffusion-limited reactions were found to be more prominent in high polarity solvents, namely dimethyl sulfoxide (DMSO), DMF, ACN, methanol, ethanol, propanol and DCM. The relationships between quenching constant (K ) and dielectric constants (ε) of the different solvents were studied.

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Section: Resultssupporting

confidence: 76%

Bimolecular fluorescence quenching reactions of the biologically active coumarin composite 2‐acetyl‐3H‐benzo[f]chromen‐3‐one in different solvents

Koppal

Melavanki

Kusanur³

et al. 2018

Luminescence

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“…Figure 6A and B show the plots of F 0 /F for Lyz versus [Q] of RxAc and RxAcNPs at 298, 304, and 310 K and pH 7.4, where [Q] ranges from 2 to 16 μMof RxAc and RxAcNPs. Plots in Figure 6A and B show that the results of Lyz-RxAc and Lyz-RxAcNPs systems agree very well with the Stern-Volmer equation, which indicates that a single type of quenching mechanism is involved, either static or dynamic [56][57][58][59]. The results listed in Table 4 showed that K SV and K q values of Lyz-RxAc and Lyz-RxAcNPs decreased upon increasing temperature and that the quenching of both systems follows the static quenching mechanism [53,60].…”

Section: Fluorescence Spectroscopysupporting

confidence: 56%

Influence of Tween 80 Surfactant on the Binding of Roxatidine Acetate and Roxatidine Acetate–loaded Chitosan Nanoparticles to Lysozyme

Qashqoosh¹,

Alahdal²,

Manea³

et al. 2022

Surfactants and Detergents - Updates and New Insights

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The drug binding to protein is an attractive research topic. In order to assess the release of RxAc-CsNPs and their binding with lysozyme under physiological conditions, nanocomposite materials based on chitosan (Cs) and Roxatidine acetate (RxAc) in the presence Tween 80 (Tw80) surfactant were developed. The addition of Tw80 to CsNPs increased RxAc release in vitro. In this work, Stern–Volmer plot and thermodynamic results indicated that the mechanism of Lyz with RxAc and Lyz with RxAc-CsNPs was static mechanism and the main forces in both systems were hydrogen bonding and Van der Waals forces, which indicated that the binding reaction in both systems is spontaneous, exothermic and enthalpically driven. Synchronous fluorescence and CD results indicated that the RxAc and RxAc-CsNPs cause change in the secondary construction of Lyz. It was also found that the addition of Tw80 affects the binding constant of drug with protein. Finally, the molecular docking results have also been in accordance with the results of other techniques. Hence, the developed RxAc loaded Chitosan nanoparticles could be used as an effective strategy for designing and application of the antiulcer drugs. Altogether, the present study can provide an important insight for the future designing of antiulcer drugs.

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“…The fluorescence yield of a fluorophore may be decreased due to a variety of molecular interactions with quencher molecules such as excited‐state reactions, energy transfer, molecular rearrangements, ground‐state complex formation and collision quenching . Such a decrease in the fluorescence yield in the presence of a quencher is called fluorescence quenching.…”

Section: Resultsmentioning

confidence: 99%

The investigation of the interaction between orientin and bovine serum albumin by spectroscopic analysis

Toprak

Arık

2013

Luminescence

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In this paper, the interaction between orientin and bovine serum albumin (BSA) was examined using fluorescence and absorbance spectroscopy. The analysis of the quenching mechanism was done using Stern-Volmer plots which exhibit upward (positive) deviation. A linear response to orientin was shown in the concentration range between 3 and 50 μM. The experimental results showed the presence of a static quenching process between orientin and BSA. The thermodynamic parameters ΔH, ΔS and ΔG were also calculated and suggested that the hydrophobic and electrostatic interactions played an important role in the interaction between orientin and BSA. Furthermore, the distances between BSA and orientin were determined according to Förster non-radiation energy transfer theory. In addition, the results of the synchronous fluorescence obtained indicated that the binding of orientin with BSA could affect conformation in BSA.

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Fluorescence quenching of newly synthesized biologically active coumarin derivative by aniline in binary solvent mixtures

Cited by 22 publications

References 32 publications

Bimolecular fluorescence quenching reactions of the biologically active coumarin composite 2‐acetyl‐3H‐benzo[f]chromen‐3‐one in different solvents

Bimolecular fluorescence quenching reactions of the biologically active coumarin composite 2‐acetyl‐3H‐benzo[f]chromen‐3‐one in different solvents

Influence of Tween 80 Surfactant on the Binding of Roxatidine Acetate and Roxatidine Acetate–loaded Chitosan Nanoparticles to Lysozyme

The investigation of the interaction between orientin and bovine serum albumin by spectroscopic analysis

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