Bimolecular fluorescence quenching reactions of the biologically active coumarin composite 2‐acetyl‐3H‐benzo[f]chromen‐3‐one in different solvents

Abstract

A study on fluorescence quenching was carried out for the coumarin derivative 2-acetyl-3H-benzo[f]chromen-3-one (2AHBC) with aniline at room temperature. Efficient fluorescence quenching was observed and Stern-Volmer (S-V) plots showed upward curves from linearity in all solvents of different polarities. For the solute 2AHBC, ground state complex formation does not hold in our study. The kinetic distance (r) value was found to be greater than the encounter distance (R) and indicated that the quenching reaction was held within the sphere of action. Diffusion-limited reactions were found to be more prominent in high polarity solvents, namely dimethyl sulfoxide (DMSO), DMF, ACN, methanol, ethanol, propanol and DCM. The relationships between quenching constant (K ) and dielectric constants (ε) of the different solvents were studied.

Investigation of the Fluorescence Turn-off Mechanism, Genome, Molecular Docking In Silico and In Vitro Studies of 2-Acetyl-3H-benzo[f]chromen-3-one

“…However, the S-V plot in the transient method shows linearity in ACN + DXN solvent mixtures. It signifies that the presence of static quenching is either due to the sphere of action model or the formation of the ground-state complex. − …”

Synthesis and Tetraphenylethylene-Based Aggregation-Induced Emission Probe for Rapid Detection of Nitroaromatic Compounds in Aqueous Media

“…Generally, a linear curve indicates that either a dynamic quenching or static quenching mechanism is involved . Meanwhile, a nonlinear and upward Stern–Volmer (SV) curve for nitrobenzene reveals contribution of both dynamic and static quenching mechanisms. − The unique interaction of the probe with analytes including donor–acceptor electron transfer may illustrate the crucial quenching mechanism of fluorescence emission. Figure c shows fluorescence emission of probe 3 with various concentrations of 4-nitrophenol and the corresponding SV plot exhibits significant quenching effects on the fluorescence intensity of the probe (Figure c,d).…”

Understanding the binding interaction between phenyl boronic acid P1 and sugars: determination of association and dissociation constants using S–V plots, steady‐state spectroscopic methods and molecular docking

“…The Stern–Volmer (S–V) equation was used to calculate the bimolecular quenching rate constant and also to investigate the kinetics of the photophysical intermolecular quenching process. [ 36–38 ] Through the S–V relationship, experimental analysis of fluorescence quenching, in most instances indicated a linear dependency, which revealed pure static or dynamic quenching alone. This can be stated as (Eqn 1): where I 0 is the intensity of fluorescence for the fluorophore and I represents the fluorescence intensity of the excited fluorophore and quencher system.…”

“…The mechanism of the interaction between boronic acid and sugars through intermediates tetrahedral boronate 2 and boronate esters 3 and 4 is shown in Scheme 1. [ 2–4,19–41 ]…”