Fluorescence polarization studies on Escherichia coli membrane stability and its relation to the resistance of the cell to freeze-thawing. II. Stabilization of the membranes by polyamines

Souzu, Hiroshi

doi:10.1016/0005-2736(86)90439-6

Cited by 31 publications

(11 citation statements)

References 16 publications

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“…Clearly, more experimental evidence is needed to support this hypothesis. Another possible scenario is that induction of polyamines at chilling temperatures could serve at least two purposes: One, to provide osmotolerance/protection to fruit membranes against oxidative stress ( Ben-Arie et al, 1982 ; Souzu, 1986 ; Ha et al, 1998 ; Groppa et al, 2001 ) and second, to induce and stabilize PR1b1 protein. Spd and Spm are known to modulate protein synthesis by inducing structural changes in RNA structure ( Igarashi and Kashiwagi, 2000 ) and stabilize proteins against degradation ( Gugliucci and Menini, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes

et al. 2016

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Plants execute an array of mechanisms in response to stress which include upregulation of defense-related proteins and changes in specific metabolites. Polyamines – putrescine (Put), spermidine (Spd), and spermine (Spm) – are metabolites commonly found associated with abiotic stresses such as chilling stress. We have generated two transgenic tomato lines (556HO and 579HO) that express yeast S-adenosylmethionine decarboxylase and specifically accumulate Spd and Spm in fruits in comparison to fruits from control (556AZ) plants (Mehta et al., 2002). Tomato fruits undergo chilling injury at temperatures below 13°C. The high Spd and Spm tomato together with the control azygous line were utilized to address role(s) of polyamines in chilling-injury signaling. Exposure to chilling temperature (2°C) led to several-fold increase in the Put content in all the lines. Upon re-warming of the fruits at 20°C, the levels of Spd and Spm increased further in the fruit of the two transgenic lines, the higher levels remaining stable for 15 days after re-warming as compared to the fruit from the control line. Profiling their steady state proteins before and after re-warming highlighted a protein of ∼14 kD. Using proteomics approach, protein sequencing and immunoblotting, the ∼14-kD protein was identified as the pathogenesis related protein 1b1 (PR1b1). The PR1b1 protein accumulated transiently in the control fruit whose level was barely detectable at d 15 post-warming while in the fruit from both the 556HO and 579HO transgenic lines PR1b1 abundance increased and remained stable till d 15 post warming. PR1b1 gene transcripts were found low in the control fruit with a visible accumulation only on d 15 post warming; however, in both the transgenic lines it accumulated and increased soon after rewarming being several-fold higher on day 2 while in 556HO line this increase continued until d 6 than the control fruit. The chilling-induced increase in PR1b1 protein seems independent of ethylene and methyl jasmonate signaling but may be linked to salicylic acid. We propose that polyamine-mediated sustained accumulation of PR1b1 protein in post-warmed chilled tomato fruit is a pre-emptive cold stress response and possibly a defense response mechanism related to Cold Stress-Induced Disease Resistance (SIDR) phenomenon.

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Section: Discussionmentioning

confidence: 99%

Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes

et al. 2016

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“…There is an increase in cardiolipin, synthesized from phosphatidylglycerol (8), and the unsaturated fatty acids are converted into their cyclopropyl fatty acid derivatives (11). Stationary-phase cells also have a higher protein/lipid ratio in their membranes, which makes them less prone to lateral phase separation (37), and a higher degree of crosslinking among membrane proteins (22). Also, the accumulation of trehalose, the increased level of stabilizing polyamines, and the thickening of the wall during stationary phase (four to five layers of peptidoglycan compared to only two or three during growth) (15,22) could contribute to the high level of stability of the cell envelopes of stationary-phase cells.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Physiological Changes Induced by High Hydrostatic Pressure in Exponential- and Stationary-Phase Cells of Escherichia coli : Relationship with Cell Death

Mañas

Mackey

2004

Appl Environ Microbiol

151

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The relationship between a loss of viability and several morphological and physiological changes was examined with Escherichia coli strain J1 subjected to high-pressure treatment. The pressure resistance of stationary-phase cells was much higher than that of exponential-phase cells, but in both types of cell, aggregation of cytoplasmic proteins and condensation of the nucleoid occurred after treatment at 200 MPa for 8 min. Although gross changes were detected in these cellular structures, they were not related to cell death, at least for stationary-phase cells. In addition to these events, exponential-phase cells showed changes in their cell envelopes that were not seen for stationary-phase cells, namely physical perturbations of the cell envelope structure, a loss of osmotic responsiveness, and a loss of protein and RNA to the extracellular medium. Based on these observations, we propose that exponential-phase cells are inactivated under high pressure by irreversible damage to the cell membrane. In contrast, stationary-phase cells have a cytoplasmic membrane that is robust enough to withstand pressurization up to very intense treatments. The retention of an intact membrane appears to allow the stationary-phase cell to repair gross changes in other cellular structures and to remain viable at pressures that are lethal to exponential-phase cells.

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“…This stabilization was recently studied, with Escherichia coli in the exponential phase of growth, using a fluorescence polarization technique (Souzu, 1986). A dose-dependent lipid immobilization was observed with spermine and it was conjectured this might be due to some bridging between integral proteins and membrane lipid binding sites and/or between lipid head-groups.…”

Section: Interaction Of Polyamines With Membrane Componentsmentioning

confidence: 99%

Influence of polyamines on membrane functions

Schuber

1989

Biochemical Journal

420

222

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Fluorescence polarization studies on Escherichia coli membrane stability and its relation to the resistance of the cell to freeze-thawing. II. Stabilization of the membranes by polyamines

Cited by 31 publications

References 16 publications

Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes

Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes

Morphological and Physiological Changes Induced by High Hydrostatic Pressure in Exponential- and Stationary-Phase Cells of Escherichia coli : Relationship with Cell Death

Influence of polyamines on membrane functions

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