Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat

Lippolis, Vincenzo; Porricelli, Anna Chiara Raffaella; Mancini, Erminia; Ciasca, Biancamaria; Lattanzio, Veronica M.T.; Girolamo, Annalisa De; Maragos, Chris M.; McCormick, Susan P.; Li, Peiwu; Logrieco, Antonio F.; Pascale, Michelangelo

doi:10.3390/toxins11070380

Cited by 18 publications

(7 citation statements)

References 33 publications

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“…For example, Lippolis and colleagues have developed a portable FP analyzer for the simultaneous determination of T-2 toxin, HT-2 toxin, and relevant glucosides in wheat [ 52 ]. Antibody used in the study showed high sensitivity (IC50 = 2.0 ng/mL) and cross-reactivity (100% for T-2 toxin and 80% for T-2 and HT-2 glucosides).…”

Section: Advance Multi-mycotoxin Immuno-based Analysismentioning

confidence: 99%

Recent advances in immunoassay-based mycotoxin analysis and toxicogenomic technologies

Liew¹,

Sabran²

2022

Journal of Food and Drug Analysis

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The co-occurrence and accumulation of mycotoxin in food and feed constitutes a major issue to food safety, food security, and public health. Accurate and sensitive mycotoxins analysis can avoid toxin contamination as well as reduce food wastage caused by false positive results. This mini review focuses on the recent advance in detection methods for multiple mycotoxins, which mainly depends on immunoassay technologies. Advance immunoassay technologies integrated in mycotoxin analysis enable simultaneous detection of multiple mycotoxins and enhance the outcomes’ quality. It highlights toxicogenomic as novel approach for hazard assessment by utilizing computational methods to map molecular events and biological processes. Indeed, toxicogenomic is a powerful tool to understand health effects from mycotoxin exposure as it offers insight on the mechanisms by which mycotoxins exposures cause diseases.

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Section: Advance Multi-mycotoxin Immuno-based Analysismentioning

confidence: 99%

Recent advances in immunoassay-based mycotoxin analysis and toxicogenomic technologies

Liew¹,

Sabran²

2022

Journal of Food and Drug Analysis

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“…Developments of the singe-reagent FPIA include Lippolis et al on the simultaneous determination of several mycotoxins in wheat [ 13 ], Mi et al on multiple determination of (fluoro)quinolone antibiotics in foodstuffs [ 14 ], and Eremin et al on the high throughput determination of aromatic compounds [ 15 ].…”

Section: Modes Of Fpiamentioning

confidence: 99%

Fluorescence Polarization-Based Bioassays: New Horizons

Hendrickson

Taranova

Zherdev

et al. 2020

Sensors

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Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the preparation of samples. For decades, these possibilities were successfully applied in fluorescence polarization immunoassays based on differences in the polarization of fluorophore emissions excited by plane-polarized light, whether in a free state or as part of an immune complex. However, the results of recent studies demonstrate the efficacy of fluorescence polarization as a detected signal in many bioanalytical methods. This review summarizes and comparatively characterizes these developments. It considers the integration of fluorescence polarization with the use of alternative receptor molecules and various fluorophores; different schemes for the formation of detectable complexes and the amplification of the signals generated by them. New techniques for the detection of metal ions, nucleic acids, and enzymatic reactions based on fluorescence polarization are also considered.

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“…Recently such strips have been adapted to test for multiple mycotoxins that include T-2 toxin [ 19 , 20 , 21 ]. Antibody-based biosensors for T-2 and HT-2 have also been reported [ 22 , 23 , 24 ], as well as a fluorescence polarization immunoassay [ 25 ] and aptamer-based tests [ 26 , 27 ]. Current trends in immunoassays for mycotoxin detection were reviewed by Nolan et al [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of T-2 Toxin in Wheat and Maize with a Portable Mass Spectrometer

Maragos

2023

Toxins

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T-2 toxin is a mycotoxin routinely found as a contaminant of cereal grains worldwide. A portable mass spectrometer was adapted to enable the detection of T-2 toxin in wheat and maize by APCI-MS. In order to facilitate rapid testing, a rapid cleanup was used. The method was able to detect T-2 toxin in soft white wheat, hard red wheat, and yellow dent maize and could be used to screen for T-2 at levels above 0.2 mg/kg. The HT-2 toxin was only detectable at very high levels (>0.9 mg/kg). Based on these results, the sensitivity was not sufficient to allow the application of the screening method to these commodities at levels recommended by the European Commission. With a cut-off level of 0.107 mg/kg, the method correctly classified nine of ten reference samples of wheat and maize. The results suggest that portable MS detection of T-2 toxin is feasible. However, additional research will be needed to develop an application sensitive enough to meet regulatory requirements.

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Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat

Cited by 18 publications

References 33 publications

Recent advances in immunoassay-based mycotoxin analysis and toxicogenomic technologies

Recent advances in immunoassay-based mycotoxin analysis and toxicogenomic technologies

Fluorescence Polarization-Based Bioassays: New Horizons

Detection of T-2 Toxin in Wheat and Maize with a Portable Mass Spectrometer

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