Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

Wang, Zhanhui; Cheng, Lin; Shi, Weimin; Zhang, Suxia; Shen, Jianzhong

doi:10.1007/s11426-010-0085-0

Cited by 11 publications

(2 citation statements)

References 6 publications

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“…Among the obtained six tracers (the homologous one (CLI‐FITC) and the heterologous ones (PAZ‐FITC, PIP‐FITC, CIP‐FITC, ENO‐FITC and NOR‐FITC)), compared with the negative one, each tracer showed a δ mP value from 90 to 199 mP, which could demonstrate sufficient binding capability with the employed antibody against clinafloxacin (Fig. ) . Thus the working concentration of these tracers in FPIA was approximately 4 nmol L −1 , as in the previously reported method …”

Section: Resultssupporting

confidence: 66%

Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk

Chen

Shanin

et al. 2015

J Sci Food Agric

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Section: Resultssupporting

confidence: 66%

Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk

Chen

Shanin

et al. 2015

J Sci Food Agric

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“…The advantage in the determination of speed makes it more suitable for screening large numbers of samples . Some of these methods have already been used for veterinary drug residues in food. − …”

Section: Introductionmentioning

confidence: 99%

One-Step Platform for Maduramicin and Salinomycin Detection Based on Bispecific Monoclonal Antibody and Interpretation of Molecular Recognition Mechanism

Huang,

Chen,

Sun

et al. 2023

J. Agric. Food Chem.

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Maduramicin (MAD) and salinomycin (SAL) are the widely used poly(ether ionophore) antibiotics to control coccidiosis in animals. Due to their strong cytotoxicity, strict control over their dosage and residue in animal food is necessary. To improve the detection efficiency of the existing single-residue detection methods, a tetraploid tumor hybrid system was constructed using drug mutagenesis, and the bispecific monoclonal antibody (BsMAb) against MAD and SAL was obtained by hybridization-hybridoma technology. By optimizing the optimal working concentration of the tracer and antibody, a multiresidue fluorescence polarization immunoassay method based on BsMAb was successfully established. The whole detection process takes 10 min, and the LOD values of MAD and SAL were 4.71 and 3.49 ng·g–1, respectively. IC50 values were 6.45 and 6.24 ng·mL–1, respectively. There was no cross-reactivity with other polyether ionophore antibiotics. Finally, a breakthrough in detection was achieved: bispecific monoclonal antibody prepared by the hybridization-hybridoma technology was used to detect maduramicin and salinomycin.

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Screening methods and recent developments in the detection of anticoccidials

et al. 2013

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This article presents a review of the current trends in the analysis of coccidiostats in various matrices, focusing principally on screening and rapid methods. Coccidiosis is an infectious disease having a high negative impact on the animal industry. Drugs are therefore necessary to prevent and/or to combat this disease. However, it is also of crucial importance that these veterinary drugs do not enter the human food chain. European legislation has therefore established the boundaries for the use of coccidiosats and has also addressed the unavoidable problem of cross-contamination of the feed, mainly caused by the use of the same production lines. Consequently there is a need for analytical methods and/or analytical strategies for the monitoring and control of the residues of anticoccidials, both in feed and in the resulting matrices for human consumption. In the frame of the European collaborative project CONffIDENCE, such attempts to establish the required analytical tools were made, which required beforehand a review of the state of the art in this domain. Aiming at this objective, in this review we consider the most interesting publications since 2000. In essence, both a rapid approach with mainly immunoassays and chromatographic methods were developed. To date, the obstacle to routine use of the first approach has been its inability to detect more than two compounds simultaneously, but recent developments in flow cytometry have made it possible to detect six coccidiostats at once. On the other hand, an increasingly popular approach for detecting multiple coccidiostats simultaneously is liquid chromatography coupled with tandem mass spectrometry. There remains a need to adapt these analytical methods to legislative requirements.

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Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

Cited by 11 publications

References 6 publications

Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk

Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk

One-Step Platform for Maduramicin and Salinomycin Detection Based on Bispecific Monoclonal Antibody and Interpretation of Molecular Recognition Mechanism

Screening methods and recent developments in the detection of anticoccidials

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