Fluorescence polarization assays for chemical contaminants in food and environmental analyses

Zhang, Huiyan; Yang, Shupeng; Ruyck, Karl De; Beloglazova, Natalia V.; Eremin, Sergei A.; Saeger, Sarah De; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui

doi:10.1016/j.trac.2019.03.013

Cited by 97 publications

(53 citation statements)

References 204 publications

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“…According to some studies [ 27 – 29 ], heterologous tracers may improve the sensitivity. Yet, many tracers built on heterologous structures will not bind to the antibody (see [ 30 ]). A preliminary guess if a tracer will bind—before performing its synthesis—is to determine the cross-reactivity of its underlying hapten in ELISA.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

et al. 2020

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Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was included in the European watch list of substances that requires its environmental monitoring in the member states. DCF may harmfully influence the ecosystem already at concentrations ≤ 1 μg L−1. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method which does not require the immobilization of reagents. FPIA can be performed in one phase within 20–30 min, making it possible to analyse wastewater without any complicated pre-treatment. In this study, new tracer molecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 μg L−1 with a working range up to 870 μg L−1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods.

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Section: Resultsmentioning

confidence: 99%

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

et al. 2020

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“…The proposed analysis versus other compact multiplex analytical systems [23] offers the following advantages:…”

Section: Discussionmentioning

confidence: 99%

Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers

Byzova

Vengerov

Voloshchuk

et al. 2019

Sensors

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The integration of several controlled parameters within a single test system is experiencing increased demand. However, multiplexed test systems typically require complex manufacturing. Here, we describe a multiplexed immunochromatographic assay that incorporates a conventional nitrocellulose membrane, which is used together with microspot printing, to construct adjacent microfluidic "tracks" for multiplexed detection. The 1 mm distance between tracks allows for the detection of up to four different analytes. The following reagents are applied in separate zones: (a) gold nanoparticle conjugates with antibodies against each analyte, (b) other antibodies against each analyte, and (c) antispecies antibodies. The immersion of the test strip in the sample initiates the lateral flow, during which reagents of different specificities move along their tracks without track erosion or reagent mixing. An essential advantage of the proposed assay is its extreme rapidity (1-1.5 min compared with 10 min for common test strips). This assay format was applied to the detection of cardiac and inflammatory markers (myoglobin, D-dimer, and C-reactive protein) in human blood, and was characterized by high reproducibility (8%-15% coefficient of variation) with stored working ranges of conventional tests. The universal character of the proposed approach will facilitate its use for various analytes.

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“…Fluorescence polarization (FP) was applied to immunoassay in 1960s and has been widely used for detection of small molecules recent years. 16,17 When the temperature and viscosity of the solution remain constant, the FP value is only related to the size of the uorescent molecule. Smaller size of uorescent molecule causes faster rotation speed, which means lower FP value.…”

Section: Introductionmentioning

confidence: 99%

A fluorescence polarization immunoassay for detection of thiacloprid in environmental and agricultural samples

et al. 2019

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Fluorescence polarization assays for chemical contaminants in food and environmental analyses

Cited by 97 publications

References 204 publications

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers

A fluorescence polarization immunoassay for detection of thiacloprid in environmental and agricultural samples

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