Fluorescence polarisation immunoassay based on a monoclonal antibody for the detection of sulphamethazine in chicken muscle

Abstract

Summary A fluorescence polarisation immunoassay (FPIA) for the screening of sulphamethazine (SMZ) in chicken muscle was developed. Fluorescein‐labelled tracers for several sulphonamides were synthesised and monoclonal antibodies (Mabs) and polyclonal antibody (Pab) were produced. The Mabs and Pabs were raised against sulphonamides conjugated to bovine serum albumin by a diazo method. The most sensitive FPIA method for SMZ detection was based on a combination of the Mab against SMZ–bovine serum albumin and tracer SMZ labelled with fluorescein isothiocyanate. The limit of detection and IC50 for SMZ were 1 and 16 ng mL−1. Recoveries of SMZ from fortified chicken muscle samples averaged between 78% and 89%. The proposed FPIA method was applied for determining SMZ in an actual residue study.

Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA

“…Sample preparation for FPIA and ic-ELISA Milk, chicken and pork samples were purchased from the local market. Samples were prepared as previously reported (S. Zhang, Wang, Irinas, Sergeia, & Shen, 2007) . In brief, aliquots of chicken and pork sample (1 g) were homogenized in 1mL PBS, respectively.…”

Investigation of Antigen-Antibody Interactions of Sulfonamides with a Monoclonal Antibody in a Fluorescence Polarization Immunoassay Using 3D-QSAR Models

“…There is an increase in polarization of the fluorescence when a small fluorescent-labeled antigen (tracer) is bound by an antibody, and the method does not use multiple steps or separations, providing simplicity of use and higher-speed. Competitive FPIAs employing specific antibodies and fluorescein-labeled antigens for determination of drug residues in food samples have been previously studied [ 19 , 20 ].…”

Application of sulphanilamides disazo dyes with Tropaeolin O for simple, rapid and sensitive spectrophotometric assay of medicines

“…For quantitative sulphanilamides assay a high-performance liquid chromatography [3][4][5][6], immunoenzyme analysis [7][8][9] and voltammetry [10,11] are often used. However, these methods are expensive in the use, require the reagents of very high purity, though their use is reasonable at the analysis of sulphanilamides rests in animal food, background objects, biological liquids.…”