Fluorescence lifetime measurements to determine the core–shell nanostructure of FITC-doped silica nanoparticles: An optical approach to evaluate nanoparticle photostability

Santra, Swadeshmukul; Liesenfeld, Bernd; Bertolino, Chiara A.; Dutta, Debamitra; Cao, Zehui; Tan, Weihong; Moudgil, Brij M.; Mericle, Robert A.

doi:10.1016/j.jlumin.2005.04.008

Cited by 92 publications

(91 citation statements)

References 40 publications

(54 reference statements)

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“…The effect of silica encapsulation on the emission lifetime and anisotropy widely varies among the three nanoparticle architectures prepared in this study ( Table 1 39 A similar phenomenon has also been reported for 1,1′-diethyl-3,3,3′,3′-tetramethylindocarbocyanine iodide doped mesoporous silica nanoparticles. 30 Since the absorption and fluorescence spectra of our Rubpy@SiO 2 NPs do not indicate that Rubpy resides in two significantly different environments within the silica matrix, we prefer the single component Lorentzian distribution of lifetimes model.…”

Section: Resultssupporting

confidence: 82%

Photophysical Properties of Dye-Doped Silica Nanoparticles Bearing Different Types of Dye−Silica Interactions

Kell

Tan

et al. 2009

J. Phys. Chem. C

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=17745c67-7bcd-486b-8b14-daecbc43474b http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=17745c67-7bcd-486b-8b14-daecbc43474b Photophysical properties of three types of dye-doped silica nanoparticles (NPs) with different dye-silica interactions have been investigated. In two cases the dye-silica interactions are noncovalent, where tris(2,2′-bipyridine)ruthenium(II) chloride (Rubpy) is attracted to the silica network electrostatically and tetramethylrhodamine-dextran (TMR-Dex) is trapped inside the silica matrix through spatial/steric hindrance. In the third case, tetramethylrhodamine-5-isothiocyanate (TRITC) modified with 3-aminopropyltriethoxysilane (APTES) to form TMR-APTES is bound to the silica matrix covalently. Although in all three types of architectures absorption, excitation, and emission spectra show only small red-shifts (<5 nm) as compared with free dye in water, excited state emission lifetimes, quantum yields, and anisotropies vary significantly and in quite different ways between the three architectures. All three types of interactions facilitate effective encapsulation of dye within a silica network. However, covalent bonding possesses a notable advantage over the other two types of interactions as it results in a large reduction of a nonradiative relaxation rate of the embedded dye (TMR-APTES) and, thus, a large (∼3.55-fold) increase of its quantum yield.

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Section: Resultssupporting

confidence: 82%

Photophysical Properties of Dye-Doped Silica Nanoparticles Bearing Different Types of Dye−Silica Interactions

Kell

Tan

et al. 2009

J. Phys. Chem. C

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“…Presently, there are several types of nanoparticle used in molecular imaging in cancer diagnosis, such as liposomes, dye-molecule-doped silica nanoparticles, Qdots, gold nanoparticles, immunotargeted nanoshells, perfluorocarbon nanoparticles, nanoshells, and magnetic nanocrystals. [67][68][69][70][71][72][73][74] Fluorescence labeling techniques have been used extensively in both biological research and clinical diagnosis. To achieve sensitive detection, there is an increasing demand for fluorescent labeling probes that are more intense, stable, and sensitive.…”

Section: Cancer Molecular Imaging Using Nanoparticles/nanoprobesmentioning

confidence: 99%

Nanomedicine for drug delivery and imaging: A promising avenue for cancer therapy and diagnosis using targeted functional nanoparticles

Liu

Miyoshi

Nakamura

2007

Intl Journal of Cancer

566

340

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The diagnosis and treatment of cancer or tumor at the cellular level will be greatly improved with the development of techniques that enable the delivery of analyte probes and therapeutic agents into cells and cellular compartments. Organic and inorganic nanoparticles that interface with biological systems have recently attracted widespread interest in the fields of biology and medicine. The new term nanomedicine has been used recently. Nanoparticles are considered to have the potential as novel intravascular or cellular probes for both diagnostic (imaging) and therapeutic purposes (drug/gene delivery), which is expected to generate innovations and play a critical role in medicine. Target-specific drug/ gene delivery and early diagnosis in cancer treatment is one of the priority research areas in which nanomedicine will play a vital role. Some recent breakthroughs in this field recently also proved this trend. Nanoparticles for drug delivery and imaging have gradually been developed as new modalities for cancer therapy and diagnosis. In this article, we review the significance and recent advances of gene/drug delivery to cancer cells, and the molecular imaging and diagnosis of cancer by targeted functional nanoparticles. ' 2007 Wiley-Liss, Inc.

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“…Dye-doped silica nanoparticles [13][14][15] (NP) stand out as excellent candidates as it is possible to dope silica NPs with a large number of fluorophores, increasing the total fluorescence of the label significantly [16,17]. Moreover, the fluorophore is protected inside a silica matrix, thereby increasing photostability [18,19] and quantum efficiency [20,21]. Silica NPs are also relatively non-toxic, chemically inert, and can be prepared in a range of sizes [22].…”

mentioning

confidence: 99%

A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

Gubala

Guével

Nooney

et al. 2010

Talanta

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When designing devices for biomedical diagnostics, increasing the signal to noise ratio is often critical for achieving clinically relevant sensitivity and limits of detection (LOD). In antibodybased assays, the measured signal can be amplified through the replacement of molecular fluorophores with doped nanoparticles (NP). However, the benefits of using NPs can only be realized if the NPs are coated efficiently with detection antibody, have good colloidal stability and the ratio of specific to non-specific binding (NSB) is high enough. The main focus of this paper is on the optimization of the bioconjugation protocol for antibody labeling of NPs leading to improved assay performance. Two types of linkers were used: monovalent linkers (glutaraldehyde; sulfo-SMCC; and sulfo-SIAB), and three generations of dendrimers endowed with multivalent carboxylic functionality. Overall, the NP-IgG conjugates prepared using multivalent linkers showed a significantly lower LOD and higher sensitivity than their homo-or hetero-functional counterparts. The multivalent dendrimers also improved NP stability and reduced aggregation. Moreover, the dendrimers showed a higher reactivity with biological material, a feature that could significantly reduce the cost of high-throughput biodiagnostics tests. Introduction:

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Fluorescence lifetime measurements to determine the core–shell nanostructure of FITC-doped silica nanoparticles: An optical approach to evaluate nanoparticle photostability

Cited by 92 publications

References 40 publications

Photophysical Properties of Dye-Doped Silica Nanoparticles Bearing Different Types of Dye−Silica Interactions

Photophysical Properties of Dye-Doped Silica Nanoparticles Bearing Different Types of Dye−Silica Interactions

Nanomedicine for drug delivery and imaging: A promising avenue for cancer therapy and diagnosis using targeted functional nanoparticles

A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

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