Fluorescence labelling with europium chelate of β-diketones and application in time-resolved fluoroimmunoassays (TR-FIA)

Advances in the study of luminescence probes for proteins

“…This method was applied to the determination of cortisol, and its detection limit was 0.5 ng/ml. Its application to immunoassay did not yield a large improvement of the sensitivity [63].…”

Design of Lanthanide Complex Probes for Highly Sensitive Time-Resolved Fluorometric Detection Methods and Its Application to Biochemical, Environmental and Clinical Analyses

“…However, fluorescence intensity was decreased by introduction of reactive group such as chlorosulfonyl group to 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (TTA) [68,69]. Therefore, a new b -diketonate ligand (CDPP) with biphenyl group as a R1 was applied to determination of analytes having a covalently binding groups to amino and phenolic hydroxyl groups [35,[59][60][61].…”

Using Ho3+Fluorescence Enhancement as a Novel Probe in Monitoring of Human Serum Albumin

“…The traditional and most widely used methods for the determination of proteins (e.g., methods of Lowry (Lowry et al 1951), brilliant blue (Zor and Selinger 1996), and amaranth (Wang, Shen, and Hao 2000)) suffer from low sensitivity, are time-consuming, or involve complicated operations. Therefore, many fluorimetric methods and fluorescence probe methods are widely applied for the detection of proteins (Bruno, Horrocks, and Zauhar 1992;Ci, Yang, and Chang 1995;Li, Li, and Tong 1996;Kessler, Meinitzer, and Wolfbeis 1997;Zhang, Zhao, and Li 2001;Yan and Marriott 2003). Among these probes, rare earth chelates have attracted extensive attention because they illustrate the rare earth luminescence characteristics (such as narrow spectral width, long luminescence lifetime, large stocks shift) and superior sensitivity.…”