Fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque

Wolff, Larry F.; Anderson, L.; Sandberg, Gregory P.; Aeppli, Dorothee; Shelburne, Charles E.

doi:10.1128/jcm.29.8.1645-1651.1991

Cited by 24 publications

(12 citation statements)

References 25 publications

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“…Each plaque sample was carried through an immunoassay as described earlier (Wolff et al 1991, Shelburne et al 1993. Briefiy, whole bacterial cells served as the solid phase in the immunoassay.…”

Section: Methodsmentioning

confidence: 99%

“…Total bacterial bound fiuorescence was determined in a fiuorimeter (Particle Concentration Fluorescence Analyzer: IDEXX Corp, Portland Me.). The relative level of species specific bacterial cells were determined by comparing the difference in fiuorescence between the control and test wells containing the plaque suspensions with the fiuorescence of a standard (Wolff et al 1991). A plaque sample was considered positive for a particular periodontal disease associated pathogen, when the relative number of bacterial cells was greater than 5 x 10^ A battery of other bacterial species are routinely included in each assay and these serve as both positive and negative controls.…”

Section: Methodsmentioning

confidence: 99%

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Prevalence of periodontal pathogens with varying metabolic control of diabetes mellitus

Tervonen

Oliver

Wolff

et al. 1994

J Clinic Periodontology

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The purpose of this study was to determine the prevalence of 5 periodontal pathogens in individuals with diabetes mellitus. Subjects (n = 107) 20-70 years of age with type 1 (n = 60) or 2 (n = 47) diabetes mellitus were studied for the occurrence of the periodontal pathogens A. actinomycetemcomitans, F. nucleatum, E. corrodens, P. gingivalis and P. intermedia. Subgingival plaque was sampled in each subject from a single site exhibiting the greatest inflammation. The evaluation of selected periodontal bacterial pathogens was based on an immunoassay utilizing bacterial specific monoclonal antibodies. 35% of the sites harbored P. gingivalis, 28% F. nucleatum and 21% E. corrodens. A. actinomycetemcomitans and P. intermedia were found in less than 10% of the sites. Subjects for whom the probing depth at the sampled site was > or = 4 mm were more often found to have detectable pathogens than those with a probing depth < or = 3 mm. Diabetic factors such as duration, type and metabolic control of the disease had no statistically significant effect on the prevalence of these bacteria.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Prevalence of periodontal pathogens with varying metabolic control of diabetes mellitus

Tervonen

Oliver

Wolff

et al. 1994

J Clinic Periodontology

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“…Several methods of detection that employ immunoassays are commercially available, including immuno¯uorescence microscopy, enzyme-linked immunosorbent assay (ELISA), membrane assays and latex agglutination assays (9,18,33,36,50,68,76,89). Overall, the methods have several important advantages including fairly low detection thresholds, they are relatively low cost, rapid, and are somewhat quantitative (64,84,85). They do not, however, permit evaluation of antibiotic sensitivity of the¯ora.…”

Section: Immunoassaysmentioning

confidence: 99%

Microbiological diagnostic testing in the treatment of periodontal diseases

Loomer¹

2004

Periodontology 2000

113

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A variety of microbiological diagnostic tests are available for clinicians to use for evaluation of patients with periodontal disease. Each one has its own unique set of advantages and disadvantages, and probably the most useful information for the clinician can be obtained using a combination of the various analytic methods. The tests appear to have their greatest utility when used on patients with chronic or aggressive periodontitis who do not respond favorable to conventional mechanical therapy. The major limitation of all microbiological tests is that the information obtained is relevant to the site sampled, and may not be representative of the microflora of the entire dentition. However, since it is often only specific sites that do not respond to initial therapy, knowing the constituents of the microflora that populate these sites is clinically relevant.

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“…Bacterial concentration fiuorescence immunoassay (BCFIA) used for screening bacteria in piaque samples The BCFIA has been described in detail in an earlier report (Wolff et al 1991). Immunological evaluation of plaque samples using the BCFIA was performed ill a specially designed 96-well microtiter plate (Fluoricon Assay Plate, IDEXX Corp., Portland, ME).…”

Section: Subjects Sites Plaque Sampling and Clinical Measurementsmentioning

confidence: 99%

“…These studies have primarily used cultivable fiora methods to screen for bacterial species in plaque. As part of an ongoing large sample study, the present investigation utilized an immunoassay to screen plaque samples for five bacterial pathogens {Wolff et al 1991). The immunoassay, which takes advantage of species-specific monoclonal antibodies (MAbs) directed against plaque pathogens {Shelburne et al 1993), was used to detemiine the natural distribution of five periodontal disease associated bacterial species as well as the relationship of these microorganisms to each other and probing depth.…”

mentioning

confidence: 99%

Natural distribution of 5 bacteria associated with periodontal disease

Wolff

Aeppli

Pihlstrom

et al. 1993

J Clinic Periodontology

107

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The purpose of this study was to determine the prevalence and distribution of 5 bacterial pathogens in subgingival plaque, their relationship with each other and probing depth. Plaque was collected from 6905 sites in 938 subjects. A bacterial concentration fluorescence immunoassay and bacterial specific monoclonal antibodies were used to determine the presence and level of P. gingivalis (Pg), A. actinomycetemcomitans (Aa), P. intermedia (Pi), E. corrodens (Ec) and F. nucleatum (Fn) in each plaque sample. The prevalence in subjects was lowest for Pg (32%) and highest for Ec (49%). The site-based frequency distribution of these bacterial species ranged from 10.3% for Pg to 18.7% for Ec. Pi and Ec were the bacterial combination most often found together in a subject (27.2%). While 64.0% of the sites were without any of the 5 bacterial species evaluated, 20.2% had only 1 of the 5 bacterial species evaluated. The remaining 15.8% of sites had at least 2 bacteria species present. There was a general linear association of the detection level of bacterial species and probing depth. The odds ratios were 3.9 (Pg), 3.0 (Aa), 4.0 (Pi), 2.7 (Ec) and 2.8 (Fn) of finding high levels of these bacterial pathogens at > 5 mm probing depth (p < or = 0.01). Mean probing depth at molar sites without a specific bacteria was greater (p < or = 0.01) in subjects with a specific bacterium compared to molar sites in subjects without the bacteria.(ABSTRACT TRUNCATED AT 250 WORDS)

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Fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque

Cited by 24 publications

References 25 publications

Prevalence of periodontal pathogens with varying metabolic control of diabetes mellitus

Prevalence of periodontal pathogens with varying metabolic control of diabetes mellitus

Microbiological diagnostic testing in the treatment of periodontal diseases

Natural distribution of 5 bacteria associated with periodontal disease

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