Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews

Garcez, Aguinaldo Silva; Barros, L.C.; Fernandes, Marcella Ueda R.; Fujii, Denise Nami; Suzuki, Selly Sayuri; Nepomuceno, Rafael

doi:10.1016/j.pdpdt.2020.101707

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…One method to analyse images and to transfer the data from visual inspection into objective measurable results is the use of image analysis with Im-ageJ software [45,46]. ImageJ software enables process-Skin Pharmacol Physiol 2021;34:281-299 DOI: 10.1159/000514498 ing of images and image analysis for various disciplines, for example, in biology, medicine, or biochemistry [47][48][49][50][51][52][53][54][55][56]. The extensive use of the ImageJ tool for image analysis in other disciplines and the broad spectrum of analysis tools being available in ImageJ software encouraged us to investigate if the software tool can also be used to evaluate the dermal penetration efficacy of curcumin.…”

Section: Theoretical Aspects For the Development Of The Skin Penetration Modelmentioning

confidence: 99%

Dermal Penetration Analysis of Curcumin in an ex vivo Porcine Ear Model Using Epifluorescence Microscopy and Digital Image Processing

Pelikh

Pinnapireddy

Keck

2021

Skin Pharmacol Physiol

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Introduction: Curcumin is a promising drug candidate, but its use for dermal application is limited due to its poor aqueous solubility. Thus, formulations that increase the solubility of curcumin are needed to fully exploit the therapeutic potential of curcumin. Various previous studies address this issue, but a comparison of the efficacy between these formulations remains difficult. The reason for this is a missing standard formulation as benchmark control and an easy-to-use skin penetration model that allows for a fast discrimination between different formulations. Objective: Thus, the aims of this study were the development of a curcumin standard formulation and a screening tool that allows for a fast discrimination between the dermal penetration efficacies of curcumin from different formulations. Methods: Ethanolic curcumin solutions were selected as simple and easy to produce standard formulations, and the ex vivo porcine ear model, coupled with epifluorescence microscopy and subsequent digital image analysis, was utilized to determine the dermal penetration efficacy of curcumin from the different formulations. Results: Results show that the utilized skin penetration model is a suitable and versatile tool that enables not only a fast determination of the dermal penetration efficacy of curcumin from different formulations but also a detailed and mechanistic information on the fate of chemical compounds after dermal penetration. Ethanolic solutions containing 0.25% curcumin were found to be the most suitable standard formulation. Conclusions: Results of the study provide a new, effective screening tool for the development of dermal formulations for improved dermal delivery of curcumin.

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Section: Theoretical Aspects For the Development Of The Skin Penetration Modelmentioning

confidence: 99%

Dermal Penetration Analysis of Curcumin in an ex vivo Porcine Ear Model Using Epifluorescence Microscopy and Digital Image Processing

Pelikh

Pinnapireddy

Keck

2021

Skin Pharmacol Physiol

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“…Similarly, in our study, the existence of micro ora was observed on the surface of TADs. Previous studies also observed the adhesion, aggregation, and development of the microbial colonization process in TADs using cell growth methods or uorescence images [13,24]. The interactions between the microorganisms and the host maintain the microecological balance around the TADs [9].…”

Section: Discussionmentioning

confidence: 95%

“…Ferreira et al utilized a scanning electron microscope (SEM) to observe the microbiota attached to the surface of the TADs, revealing bio lm existence on the head, transmucosal, and body segments of the TADs. With the utilization of uorescence imaging, Garcez et al observed higher uorescent intensity in in amed TADs with redness on surrounding tissues [13]. When exploring the differences of microbiome between stable TADs and unstable TADs, nevertheless, polymerise chain reaction (PCR) and DNA-DNA hybridization revealed no major differences in the testi ed microbiome.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Oral Microbiome on Temporary Anchorage Devices Under Different Oral Hygiene Conditions

Zhao

Zhang

Guo

et al. 2021

Preprint

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Background: Temporary anchorage devices (TADs) are maximum anchorages that have been widely used in orthodontic treatment. Poor oral hygiene might result in inflammation and decreased stability of the TADs. In this study, we aimed to unveil the differences in the microbiome between TADs under different oral hygiene conditions.Methods: Oral hygiene condition was stratified by Oral Hygiene Index- Simplified (OHI-S), Plaque Index (PLI), and Gingival Inflammation. Scanning electron microscopy (SEM) was used to analyze the existence of biofilm on the surface of 8 TADs, Ten TADs from the good oral hygiene group (GOH), and 10 TADs from the poor oral hygiene group (POH) were analyzed by 16S rRNA gene sequencing. Results: Principal coordinate analysis (PCoA) based on β diversity revealed differential sample clusters depending on oral hygiene conditions. When comparing specific genera, Veillonella, Streptococcus, Neisseria, were more enriched in the poor oral hygiene group. Conversely, Fusobacterium, Porphyromonas exhibited more richness in the good oral hygiene group. TADs in the good oral hygiene group demonstrated enriched microbial activities involved with signal transduction, cell mobility and energy metabolism. TADs in poor oral hygiene demonstrated enriched functions in membrane transport, transcription and signaling molecules and interactions. Conclusions: In summary, this analysis elucidated the difference in total composition and function of TADs oral microorganisms between patients with good oral hygiene and patients with poor oral hygiene, which highlighted the importance of maintaining good oral hygiene in TADs treatment.

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Failure of Orthodontic Temporary Anchorage Devices

Yan,

Zhang,

et al. 2023

Clinical Insertion Techniques of Orthodontic Temporary Anchorage Devices

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Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews

Cited by 5 publications

References 26 publications

Dermal Penetration Analysis of Curcumin in an ex vivo Porcine Ear Model Using Epifluorescence Microscopy and Digital Image Processing

Dermal Penetration Analysis of Curcumin in an ex vivo Porcine Ear Model Using Epifluorescence Microscopy and Digital Image Processing

Analysis of Oral Microbiome on Temporary Anchorage Devices Under Different Oral Hygiene Conditions

Failure of Orthodontic Temporary Anchorage Devices

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