Fluorescence in situ hybridization analysis of matched primary tumour and lymph‐node metastasis of D1 (pT2–3pN1M0) prostate cancer

Abstract

nuclei obtained from matched primary tumours and their lymph node metastases. RESULTSOf the 28 suitable cases it was possible to complete the study in 18 pairs of matched tissues; the remainder were excluded because of insufficient tissue or poor preservation of at least one of the tissues. There was cytogenetic change (aneuploidy) in 16 of the 18 primary tumours, the most common being monosomy 8, detected in 14, followed by trisomy 7, in 13 aneuploid tumours. All lymph node metastases were aneuploid by FISH. As in the primary tumours, monosomy 8 and trisomy 7 were the most common cytogenetic alterations, in 13 and 15 of the lymph node tissues. FISH analysis showed a high correlation (83%) in the cytogenetic pattern of changes between the primary tumours and their lymph node metastases. Moreover, a similar number of cells had the most common aneusomies when comparing prostate and the lymph node tissues. CONCLUSIONSThese results show a similar pattern of cytogenetic alteration in the primary tumour and its lymph node metastasis, characterized by the frequent presence of trisomy 7 and monosomy 8, suggesting that clonal cell selection is not involved in the metastatic process.

Biological resonance for cancer metastasis, a new hypothesis based on comparisons between primary cancers and metastases

“…On the one hand, some studies showed that metastases were high similar to their matched primary cancers at the levels of cytologic features [25,26], cytogenetic alterations [27][28][29][30][31][32][33][34][35], DNA changes including aneuploidy, and index [36][37][38][39][40][41][42], gene analyses including mutations [43][44][45][46][47][48][49][50][51][52][53], profiles [18,[54][55][56][57], and epigenetic and transcriptive changes [58][59][60][61][62], proteomic profiling [61,63] and expression [64][65][66][67][68][69][70][71]…”