Fluorescence exclusion – a rapid, accurate and powerful method for measuring yeast cell volume

Abstract

Cells exist in an astonishing range of volumes across and within species. However, our understanding of cell size control remains limited, due in large part to the challenges associated with accurate determination of cell volume. Much of our comprehension of size regulation derives from yeast models, but even for these morphologically stereotypical cells, assessment of cell volume has mostly relied on proxies and extrapolations from two-dimensional measurements. Recently, the fluorescence exclusion method (FXm) was developed to evaluate the size of mammalian cells, but whether it could be applied to smaller cells remained unknown. Using specifically designed microfluidic chips and an improved data analysis pipeline, we show here that FXm reliably detects subtle differences in the volume of fission yeast cells, even for those with altered shapes. Moreover, it allows for the monitoring of dynamic volume changes at the single-cell level with high time resolution. Collectively, our work highlights how the coupling of FXm with yeast genetics will bring new insights into the complex biology of cell growth.

Sodium Dynamics in the Cellular Environment

“…The measured diffusion coefficients are shown in Figure S8. The diffusion length is smaller than the size of a typical yeast cell . Thus, the effect of restricted diffusion is not obvious.…”

“…The diffusion length D 6 0.55 m in is smaller than the size of a typical yeast cell. 78 Thus, the effect of restricted diffusion is not obvious.…”