Fluorescence dye as novel label molecule for quantitative SERS investigations of an antibiotic

Abstract

Within this contribution, the proof-of-principle for a new concept for indirect surface-enhanced Raman spectroscopy (SERS) detection is presented. The fluorescence dye FR-530 is applied as a label molecule for the antibiotic erythromycin. The antibiotic binds directly to the label molecule. Changes within the SERS spectrum of the fluorescence dye appearing with the presence of the antibiotic are utilized for the detection and quantitative investigations of erythromycin. With the new concept of binding the label molecule directly to the analyte molecule, the application of linkage compounds like antibodies or any other recognition molecules becomes dispensable.

Plasmonic nanostructures for surface enhanced spectroscopic methods

“…microfluidic lab-on-a-chip devices. [179][180][181][182][183] Liu et al employed citrate-reduced AgNPs in a microdroplet PDMS detector for trace level crystal violet detection with a LoD of 3.6 nM. 184 A SEF-based immunoassay employing silver NPs produced by the Lee-Meisel protocol was presented by Zhang et al 185 Within this investigation a glass slide with several polyelectrical layers was covered with BSA (bovine serum albumin) and antigens before binding silver-antibody nanoconjugates (SANC).…”

Bioanalytical application of surface‐ and tip‐enhanced Raman spectroscopy

“…With increasing mercury(II) ion concentrations, the SERS signal intensity decreased as rhodamine B was replaced on the surface of the SERS‐active substrate . The segmented flow technique for LOC‐SERS was applied for online monitoring of nicotine , the detection of an antibiotic via a label molecule , the determination of the enzymatic activity of thiopurine methyltransferase in lysed red blood cells , and the discrimination of bacteria on strain level . Moreover, a two‐phase flow microfluidic system was used for an ultrafast SERS detection .…”

“…Beside the application of microfluidic LOC‐SERS for the detection of several bioanalytical tasks such as the determination of enzymatic activity , the characterization of cellular chemical dynamics , the specific determination of DNA , drugs, and pharmaceuticals , as well as bacteria , the microfluidic devices are also used for the preparation of SERS‐active substrates. In some cases, the synthesis of colloids is integrated into the SERS detection chip but there are also applications, where the microfluidic device is especially designed and used to generate the SERS‐active substrate.…”

P5.6 - Anwendung SERS-aktiver Nanostrukturen in der Medikamentenüberwachung und Lebensmittelanalytik

“…Durch die Kombi-nation dieser hochspezifischen und sensitiven Methode mit mikrofluidischen Funktionselementen können spektroskopische Untersuchungen im Hochdurchsatz durchgeführt und eine hohe Reproduzierbarkeit gewährleistet werden [4]. Beispielsweise sind tropfenbasierte, mikrofluidische Lab-on-a-chip-Ansätze verfügbar [5][6][7][8]. Über Dosieranschlüsse werden wässrige Tropfen in einem Ölstrom generiert, die den Analyten, die SERS-aktiven Silbernanopartikel und das Aktivierungsagenz enthalten.…”