Fluorescence diffuse tomography technique with autofluorescence removal based on dispersion of biotissue optical properties

Kleshnin, Mikhail; Turchin, Ilya V.

doi:10.1088/1612-2011/10/7/075601

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…Another way to compete the image blurring and to improve the localization of fluorophores is to employ tomographic approaches for image formation combined with sophisticated image processing tools [ 60 ]. However, the developed techniques—such as fluorescence diffuse tomography [ 13 , 61 ] and multispectral fluorescence tomography [ 17 ]—are time-consuming, and are mainly applied in small animal studies due to the low signal level.…”

Section: Discussionmentioning

confidence: 99%

“…Most fluorescent dyes and proteins exhibit narrow excitation and emission bands, and multispectral fluorescence imaging is primarily utilized for the subtraction of autofluorescence background and spectral unmixing of different fluorophores [ 15 , 16 , 17 ]. At the same time, a fluorophore with a wideband excitation spectrum or several pronounced absorption peaks in different bands of the visible and NIR ranges has the potential to utilize optical absorption dispersion for assessment of the fluorophore localization depth.…”

Section: Introductionmentioning

confidence: 99%

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Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Kirillin

Khilov

Kurakina

et al. 2021

Cancers

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Fluorescence imaging modalities are currently a routine tool for the assessment of marker distribution within biological tissues, including monitoring of fluorescent photosensitizers (PSs) in photodynamic therapy (PDT). Conventional fluorescence imaging techniques provide en-face two-dimensional images, while depth-resolved techniques require complicated tomographic modalities. In this paper, we report on a cost-effective approach for the estimation of fluorophore localization depth based on dual-wavelength probing. Owing to significant difference in optical properties of superficial biotissues for red and blue ranges of optical spectra, simultaneous detection of fluorescence excited at different wavelengths provides complementary information from different measurement volumes. Here, we report analytical and numerical models of the dual-wavelength fluorescence imaging of PS-containing biotissues considering topical and intravenous PS administration, and demonstrate the feasibility of this approach for evaluation of the PS localization depth based on the fluorescence signal ratio. The results of analytical and numerical simulations, as well as phantom experiments, were translated to the in vivo imaging to interpret experimental observations in animal experiments, human volunteers, and clinical studies. The proposed approach allowed us to estimate typical accumulation depths of PS localization which are consistent with the morphologically expected values for both topical PS administration and intravenous injection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Kirillin

Khilov

Kurakina

et al. 2021

Cancers

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“…At present, both methods are in the stage of testing in numerical phantoms. The interest of the researchers from IAP RAS is mainly concentrated on diffuse fluorescence molecular tomography (DFMT) in small laboratory animals, using the frequency-domain technique of tissue probing and signal registration [159][160][161]. However, they have also the experience in the field of experimental mammography [143].…”

Section: Diffuse Optical Tomographymentioning

confidence: 99%

“…High-quality diffusion tomograms using the own original reconstruction methods are obtained also by the Russian researchers from the Biophotonics Laboratory, Institute of Applied Physics of the Russian Academy of Sciences (IAP RAS, Nizhny Novgorod) [143,[159][160][161] Acknowledging the achievements of Russian scientists in DOT, we should mention also the development of methods for in vitro and in vivo measurement of tissue optical parameters [1,[162][163][164][165][166][167][168][169][170][171]. From the point of view of DOT methodological base, the knowledge of optical parameters is necessary for constructing adequate spatial optical models of tissues and for testing the methods of diffusion tomogram reconstruction at the stages of numerical and physical experiments, as well as in clinical testing.…”

Section: Introductionmentioning

confidence: 99%

Diffuse optical mammotomography: state-of-the-art and prospects

Konovalov

Genina

Bashkatov

2016

JBPE

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Abstract:The principles of diffuse optical tomography (DOT) of tissues are presented. The DOT capabilities as a method of breast cancer diagnostics are analysed. The state-ofthe-art of the DOT instrumentation and methodological base in application to solving the mammography problems are described. The significant contribution of Russian scientists to the development of the DOT methodology is emphasised. Basing on the results of the analysis, the authors expect the possibility of soonest entry of diffuse optical mammotomographs to the market of medical imaging instrumentation, and the capability of Russian researchers to take part in the competition for this market. © 2016 Journal of Biomedical Photonics & Engineering.Key words: diffuse optical tomography, breast, optical and functional parameters, methods of determining optical parameters, methods of image reconstruction, perturbation model of reconstruction, temporal point spread function, spatial resolution, reconstruction time. University Press, Cambridge (2016). 7. S. R. Arridge, and M. Schweiger, "Inverse methods for optical tomography," in Information Processing in Medical Imaging, H.H. Barrett (ed.), Springer-Verlag, Flagstaff, 259-277 (1993). 8. J. C. Hebden, S. R. Arridge, and D. T. Delpy, "Optical imaging in medicine: I. Experimental techniques,"Phys. Med. Biol. 42, 825-840 (1997). 9. S. R. Arridge, and J. C. Hebden, "Optical imaging in medicine: II. Modelling and reconstruction," Phys. Med. Biol. 42, 841-853 (1997). 10. B. B. Das, F. Liu, and R. R. Alfano, "Time-resolved fluorescence and photon migration studies in biomedical and random media," Rep. Prog. Phys. 60, 227-292 (1997). 11. K. Michielsen, H. De'Raedt, J. Przeslawski, and N. Garcia, "Computer simulation of time-resolved optical imaging of objects hidden in turbid media," Phys. Rep. 304, 89-144 (1998). 12. S. R. Arridge, "Optical tomography in medical imaging," Inverse Problems 15, R41-R93 (1999). Darzi, "Diffuse optical imaging of the healthy and diseased breast: a systematic review," Breast Cancer Research and Treatment 108, 9-22 (2008). 19. S. L. Jacques, and B. W. Pogue, "Tutorial on diffuse light transport," J. Biomed. Opt. 13, 041302 (2008 Biol. 361, 171-179 (1994). 31. A. Yodh, and B. Chance, "Spectroscopy and imaging with diffusing light," Phys. Today 48, 34-40 (1995). 32. J. C. Hebden, S. R. Arridge, "Imaging through scattering media by the use of an analytical model of perturbation amplitudes in the time domain," Appl. Opt. 35, 6788-6796 (1996). 33. K. D. Paulsen, and H. Jiang, "Enhanced frequency-domain optical image reconstruction in tissues through total-variation minimization," Appl. Opt. 35, 3447-3458 (1996). 34. R. J. Grable, "Optical tomography improves mammography," Laser Focus World 32, 113-118 (1996). 35. V. V. Lyubimov, "The physical foundation of the strongly scattering media laser tomography," Proc. SPIE 2769SPIE , 107-110 (1996. 36. V. V. Lyubimov, "Optical tomography of highly scattering media by using the first transmitted photons of ultrashort pulses," Optics a...

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“…Optical imaging of biological tissues is a highly informative, non-invasive and inexpensive method of analyzing normal and pathological biological tissue sites [1][2][3][4][5][6]. Labelling these tissue sites with luminescent biocomplexes, often referred to as molecular probes, improves localization accuracy and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique

Khaydukov¹,

Семчишен²,

Семиногов³

et al. 2014

Laser Phys. Lett.

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The new generation of synthetic nanomaterials, upconversion nanoparticles (UCNPs), have the potential for high-contrast optical imaging of biological tissue by virtue of their unique luminescent properties which enable the autofluorescence and excitation signals to be completely suppressed and avoid biotissue absorption. The potential for deep tissue imaging, such as whole animal imaging, is demonstrated in this report on a comparative study of two epiluminescent imaging methods suitable for the localization of a UCNP-labelled pathology site buried in highly scattering biological tissue modelled by an optical tissue phantom. The lateral resolution exhibited in scanning imaging by an illumination-collection fibre-optic probe appeared to be almost 1.73 times better than that shown by the wide-field CCD commonly used in diffuse optical tomography systems. We attribute this improved lateral resolution to the enhanced angular selectivity of the illumination-collection regime and to the nonlinear dependence of the UCNP luminescence on the excitation intensity.

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Fluorescence diffuse tomography technique with autofluorescence removal based on dispersion of biotissue optical properties

Cited by 9 publications

References 37 publications

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Diffuse optical mammotomography: state-of-the-art and prospects

Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique

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